Abstract
Some cancer cells form highly regulated structures, termed invadopodia, which mediate local, enzymatic degradation of extracellular matrix and facilitate cancer cell invasion and migration during metastatic progression. Understanding invadopodium formation and function in cancer cells is therefore an important strategy to find novel clinical approaches to interfere with metastasis. Invadopodia are F-actin-rich protrusions that form on the advancing edge of cells, supported by complex molecular interactions at the cell membrane. Invadopodia formation, structure, and function can be studied in vitro, using commonly cultured cancer cell lines and standard microscopic techniques. Here, these approaches are described in detail.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Clarke, M.J., Battagin, S., Coppolino, M.G. (2024). Assessment of Invadopodium Formation and Gelatin Degradation in Vitro. In: Santamaria, S. (eds) Proteases and Cancer. Methods in Molecular Biology, vol 2747. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3589-6_12
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DOI: https://doi.org/10.1007/978-1-0716-3589-6_12
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3588-9
Online ISBN: 978-1-0716-3589-6
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