Abstract
Cancer cells possess a remarkable capacity to dissociate from a primary tumor, invade the surrounding tissues and vasculature, and eventually form metastases in distant organs. This complex and multistep process remains one of the major causes of mortality in cancer patients worldwide. Multiple studies have highlighted the role of actin-rich structures called invadopodia (“invasive feet”), which adhere to the matrix, contain and secrete matrix-degrading proteinases, and apply protrusive forces generated by the actin cytoskeleton, which drive the invasive process. Here, we describe a fluorescent microscopy-based protocol for imaging and quantifying both invadopodia formation and matrix degradation.
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Acknowledgments
The studies described herein are funded by the Israel Science Foundation. The authors are grateful to Dr. Or-Yam Revach for her contribution to the development of the assay in our laboratory.
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Venghateri, J.B., Geiger, B. (2023). Quantification of Invadopodia Formation and Matrix Degradation Activity. In: Zaidel-Bar, R. (eds) Mechanobiology. Methods in Molecular Biology, vol 2600. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2851-5_12
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DOI: https://doi.org/10.1007/978-1-0716-2851-5_12
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2850-8
Online ISBN: 978-1-0716-2851-5
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