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Proteases and Cancer

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Salvatore Santamaria

    1. Department of Biochemical Sciences, School of Biosciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, UK

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2747)

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About this book

This volume details bioinformatics analysis, biochemical assays, recombinant protein expression and purification, methods to investigate protease activity in cell-based, organoids and in vivo systems, proteomics, transcriptomics, machine learning and novel approaches to target dysregulated protease activity in cancer.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Cancer Immunotherapy: Methods and Protocols aims to provide the scientific community with a diverse and representative collection of state-of-the-art methods to investigate the protean functions of proteases in cancer biology.

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Web-Based Resources to Investigate Protease Function

    • Salvatore Santamaria
    Pages 1-18

  3. N-Terminomics/TAILS of Human Tumor Biopsies and Cancer Cell Lines

    • Afshin Derakhshani, Mitchell Bulluss, Regan Penner, Antoine Dufour
    Pages 19-28

  4. A New Affinity-Based Probe to Profile MMP Active Forms

    • Carole Malgorn, François Becher, Pierrick Bruyat, Carole Fruchart-Gaillard, Fabrice Beau, Sarah Bregant et al.
    Pages 29-39

  5. N-Glycan Insertion for Probing Protein–Protein Interactions and Epitope Mapping

    • Shayma Abukar, Xiaohan Zhang, Bertina Dragūnaitė, Gwladys Chabrier, Rens de Groot
    Pages 41-53

  6. Expression and Purification of Recombinant ADAMTS8

    • Tina Burkhard, Alexander Frederick Minns, Salvatore Santamaria
    Pages 55-66

  7. Expression and Purification of Active Monomeric MMP7

    • Kazuhiro Yamamoto, Moe Isohata, Shouichi Higashi
    Pages 67-73

  8. Mapping the Binding Sites of MMPs on Types II and III Collagens Using Triple-Helical Peptide Toolkits

    • Szymon W. Manka
    Pages 75-82

  9. Determination of Versikine Levels by Enzyme-Linked Immunosorbent Assay (ELISA)

    • Alexander Frederick Minns, Salvatore Santamaria
    Pages 83-93

  10. Methods to Investigate Thrombin Cleavage of Osteopontin (OPN)

    • Lei Zhao, Lawrence L. Leung, John Morser
    Pages 95-117

  11. Using siRNA Silencing to Analyze ADAM17 in Macrophages

    • Matthew Markham, Linda Troeberg
    Pages 119-129

  12. Preparation and Characterization of Collagen–Hyaluronic Acid (Col–HA) Matrices: In Vitro Mimics of the Tumor Microenvironment

    • Sarbajeet Dutta, Shamik Sen
    Pages 131-139

  13. Assessment of Invadopodium Formation and Gelatin Degradation in Vitro

    • Marguerite J. Clarke, Samantha Battagin, Marc G. Coppolino
    Pages 141-149

  14. Investigation of MT1-MMP Activity in Cancer Cells

    • Yoshifumi Itoh
    Pages 151-166

  15. Gelatin In Situ Zymography to Study Gelatinase Activity in Colon Cancer Cells Treated with Platelet Microparticles (PMPs)

    • Jakub Kryczka, Hassan Kassassir, Izabela Papiewska-Pająk, Joanna Boncela
    Pages 167-176

  16. Interrogating the Impact of Protease Activity on Tumor Progression Using 3D Spheroid Models

    • Shayin V. Gibson, Edward P. Carter, Richard P. Grose
    Pages 177-188

  17. Analysis of Matrix Metalloproteinase Activity and Inhibition in Cancer Spheroids

    • Anna M. Knapinska, Lillian Onwuha-Ekpete, Gary Drotleff, Destiny Twohill, Cedric Chai, Alexa Ernce et al.
    Pages 189-209

  18. Intravital Microscopy to Study the Effect of Matrix Metalloproteinase Inhibition on Acute Myeloid Leukemia Cell Migration in the Bone Marrow

    • Floriane S. Tissot, Sara Gonzalez-Anton, Cristina Lo Celso
    Pages 211-227

  19. Fluorescence-Based Peptidolytic Assay for High-Throughput Screening of MMP14 Inhibitors

    • Hyun Lee, Lucas Ibrahimi, Kyu-Yeon Han
    Pages 229-242

  20. Generation of Protease Inhibitory Antibodies by Functional In Vivo Selection

    • Ki Baek Lee, Xin Ge
    Pages 243-256
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Editors and Affiliations

  • Department of Biochemical Sciences, School of Biosciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, UK

    Salvatore Santamaria

Bibliographic Information

  • Book Title: Proteases and Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Salvatore Santamaria

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3589-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3588-9Published: 02 December 2023

  • Softcover ISBN: 978-1-0716-3591-9Due: 15 December 2024

  • eBook ISBN: 978-1-0716-3589-6Published: 01 December 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 316

  • Number of Illustrations: 20 b/w illustrations, 60 illustrations in colour

  • Topics: Cancer Research

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