Abstract
During the metastatic process, carcinoma cells form invadopodia, F-actin enriched protrusive structures, to degrade the extracellular matrix (ECM) in order to invade the surrounding stroma and intravasate into the circulatory system. In this chapter, we describe the 2D-fluorescent matrix degradation assay, a highly sensitive and reproducible in vitro method used to measure invadopodia-mediated ECM degradation. We provide a detailed protocol on how to prepare the glass coverslips with fluorescent gelatin matrix and a standardized method to quantify gelatin degradation and invadopodia formation in order to evaluate cell invasion.
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Acknowledgments
This work was supported by an American-Italian Cancer Foundation Post-Doctoral Research Fellowship to R.F. and grants from NCI (1R01CA174869, 1R01CA206880, and 1R01CA236386) and California Tobacco-Related Disease Research Program (TRDRP-587107) to J.Y.
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Fontana, R., Yang, J. (2021). Matrix Degradation Assay to Measure the Ability of Tumor Cells to Degrade Extracellular Matrix. In: Stein, U.S. (eds) Metastasis. Methods in Molecular Biology, vol 2294. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1350-4_11
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DOI: https://doi.org/10.1007/978-1-0716-1350-4_11
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