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A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix

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Melanoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2265))

Abstract

In order to protrude within a dense tissue, tumor cells have to develop the ability to digest the extracellular matrix (ECM). Melanoma cells, similarly to other types of tumor cells, form invadopodia, membranous invaginations rich in filamentous actin and several other proteins including matrix metalloproteinases (MMPs). MMPs degrade ECM structural proteins such as collagens, fibronectin, or laminin. Here we describe an assay that allows the detection of gelatinase activity exhibited by tumor cells under 2D conditions and methods to present obtained data in both a quantitative and a qualitative manner.

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Acknowledgments

This research was funded by National Science Centre, Poland, grant numbers 2015/17/B/NZ3/03604 (Opus 9, granted to A.J.M.) and 2016/22/E/NZ3/00654 (Sonata Bis 6, granted to A.J.M.).

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Authors and Affiliations

  1. Department of Cell Pathology, Faculty of Biotechnology, University of Wroclaw, Wrocław, Poland

    Ewa Mazurkiewicz, Ewa Mrówczyńska, Aleksandra Simiczyjew, Dorota Nowak & Antonina J. Mazur

Authors
  1. Ewa Mazurkiewicz
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  2. Ewa Mrówczyńska
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  3. Aleksandra Simiczyjew
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  4. Dorota Nowak
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  5. Antonina J. Mazur
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Corresponding author

Correspondence to Antonina J. Mazur .

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Editors and Affiliations

  1. Department of Biology, Hampden-Sydney College, Hampden-Sydney, VA, USA

    Kristian M. Hargadon

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Mazurkiewicz, E., Mrówczyńska, E., Simiczyjew, A., Nowak, D., Mazur, A.J. (2021). A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix. In: Hargadon, K.M. (eds) Melanoma. Methods in Molecular Biology, vol 2265. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_3

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  • DOI: https://doi.org/10.1007/978-1-0716-1205-7_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1204-0

  • Online ISBN: 978-1-0716-1205-7

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