Abstract
The Protoporphyrin IX–Triplet State Lifetime Technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO2) and mitochondrial oxygen consumption (mitoVO2) in skin. In this work, we describe the principles of the method in small experimental animals.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Ishii N (2007) Role of oxidative stress from mitochondria on aging and cancer. Cornea 26(9 Suppl 1):S3–S9
Ballinger SW (2005) Mitochondrial dysfunction in cardiovascular disease. Free Radic Biol Med 38(10):1278–1295
Fink MP (2002) Cytopathic hypoxia. Is oxygen use impaired in sepsis as a result of an acquired intrinsic derangement in cellular respiration? Crit Care Clin 18(1):165–175
Galley HF (2011) Oxidative stress and mitochondrial dysfunction in sepsis. Br J Anaesth 107(1):57–64
Garrabou G et al (2012) The effects of sepsis on mitochondria. J Infect Dis 205(3):392–400
Mik EG et al (2006) Mitochondrial PO2 measured by delayed fluorescence of endogenous protoporphyrin IX. Nat Methods 3(11):939–945
Mik EG et al (2009) Mitochondrial oxygen tension within the heart. J Mol Cell Cardiol 46(6):943–951
Mik EG et al (2008) In vivo mitochondrial oxygen tension measured by a delayed fluorescence lifetime technique. Biophys J 95(8):3977–3990
Mik EG (2013) Measuring mitochondrial oxygen tension: from basic principles to application in humans. Anesth Analg 117(4):834–846
Harms FA et al (2013) Cutaneous respirometry by dynamic measurement of mitochondrial oxygen tension for monitoring mitochondrial function in vivo. Mitochondrion 13(5):507–514
Wefers Bettink MA et al (2020) Non-invasive versus ex vivo measurement of mitochondrial function in an endotoxemia model in rat: toward monitoring of mitochondrial therapy. Mitochondrion 50:149–157
Romers LH et al (2016) Cutaneous mitochondrial PO2, but not tissue oxygen saturation, is an early indicator of the physiologic limit of hemodilution in the pig. Anesthesiology 125(1):124–132
Ubbink R et al (2017) A monitor for cellular oxygen METabolism (COMET): monitoring tissue oxygenation at the mitochondrial level. J Clin Monit Comput 31(6):1143–1150
Harms FA et al (2012) Validation of the protoporphyrin IX-triplet state lifetime technique for mitochondrial oxygen measurements in the skin. Opt Lett 37(13):2625–2627
Golub AS, Tevald MA, Pittman RN (2011) Phosphorescence quenching microrespirometry of skeletal muscle in situ. Am J Physiol Heart Circ Physiol 300:H135–H143
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Harms, F.A., Mik, E.G. (2021). In Vivo Assessment of Mitochondrial Oxygen Consumption. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 2277. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1270-5_12
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1270-5_12
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1269-9
Online ISBN: 978-1-0716-1270-5
eBook Packages: Springer Protocols