Abstract
The protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO2) and mitochondrial oxygen consumption (mitoVO2) in skin. In this work we describe the principles of the method in experimental animals.
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References
Ishii N (2007) Role of oxidative stress from mitochondria on aging and cancer. Cornea 26(9 Suppl 1):S3–S9
Ballinger SW (2005) Mitochondrial dysfunction in cardiovascular disease. Free Radic Biol Med 38(10):1278–1295
Fink MP (2002) Cytopathic hypoxia. Is oxygen use impaired in sepsis as a result of an acquired intrinsic derangement in cellular respiration? Crit Care Clin 18(1):165–175
Galley HF (2011) Oxidative stress and mitochondrial dysfunction in sepsis. Br J Anaesth 107(1):57–64
Garrabou G et al (2012) The effects of sepsis on mitochondria. J Infect Dis 205(3):392–400
Edeas M, Weissig V (2014) Strategies, innovations & clinical applications. In: Edeas M, Weissig V (eds). Targeting mitochondria, Berlin
Mik EG et al (2006) Mitochondrial PO2 measured by delayed fluorescence of endogenous protoporphyrin IX. Nat Methods 3(11):939–945
Mik EG et al (2009) Mitochondrial oxygen tension within the heart. J Mol Cell Cardiol 46(6):943–951
Mik EG et al (2008) In vivo mitochondrial oxygen tension measured by a delayed fluorescence lifetime technique. Biophys J 95(8):3977–3990
Mik EG (2013) Measuring mitochondrial oxygen tension: from basic principles to application in humans. Anesth Analg 117(4):834–846
Harms FA et al (2013) Cutaneous respirometry by dynamic measurement of mitochondrial oxygen tension for monitoring mitochondrial function in vivo. Mitochondrion 13(5):507–514
Harms FA et al (2012) Validation of the protoporphyrin IX-triplet state lifetime technique for mitochondrial oxygen measurements in the skin. Opt Lett 37(13):2625–2627
Golub AS, Tevald MA, Pittman RN (2011) Phosphorescence quenching microrespirometry of skeletal muscle in situ. Am J Physiol Heart Circ Physiol 300:H135–H143
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Harms, F.A., Mik, E.G. (2015). In Vivo Assessment of Mitochondrial Oxygen Consumption. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_20
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DOI: https://doi.org/10.1007/978-1-4939-2257-4_20
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2257-4
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