Opinion statement
Atherosclerotic disease, a primary cause of stroke and myocardial infarction, is the most common underlying cause of death worldwide. While atherosclerosis was formerly considered to be a relatively inert structural abnormality, decades of research have since shown that it is a biologically active process, driven by active inflammation. In concert with this conceptual shift, newer strategies to image vascular lesions have evolved. 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) imaging has been validated as a non-invasive tool to characterize atherosclerotic inflammation. It is hypothesized that a combination of structural and biological (e.g., inflammatory) imaging may provide better means to assess clinical risk, to assess efficacy of therapy, and to identify new, effective treatments. Limitations remain, however, and further advances in technology and tracer development are required before FDG PET imaging will contribute a significant clinical impact at the level of the individual patient.
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Introduction
Advances in non-invasive cardiovascular imaging techniques have improved risk prediction and allow earlier detection of pathological lesions. Previously, direct visualization of the coronary arteries was limited to angiography, which is able only to diagnose obstructive lesions and is too invasive to function as a screening tool. At-risk lesions, however, may be non-obstructive and can be better identified by techniques that characterize wall morphological features, in concert with molecular markers, such as: surrounding inflammation, thrombosis, apoptosis, vessel formation, and hemorrhage [1].
This review focuses on the utility of positron emission tomography (PET)-based atherosclerotic plaque imaging using 18F-fluorodeoxyglucose (FDG) in combination with computed tomography (CT) to jointly characterize the biological and morphological features the arterial wall. The ability of PET/CT imaging to define arterial inflammation and its relationship to risk of cardiovascular disease events and the role of imaging to evaluate the impact of therapy will be discussed. Potential clinical advancements using novel tracers and a brief introduction to other non-invasive cardiac imaging modalities will be outlined.
Plaque development
A significant amount of research spanning decades has greatly enhanced our understanding of how atherosclerotic plaques develop, rupture, and precipitate atherothrombotic events. Briefly, endothelial dysfunction, a result of vascular risk factors, is associated with aggregation of lipids, monocytes, and other inflammatory cells, along the vessel wall and intima. Collections of these cells lead to atheromatous plaques with a fibrous cap which generally do not cause flow-limiting lesions; they are, however, imbued with microscopic calcifications and proteolytic enzymes released by macrophages undergoing apoptosis [2••]. Though this process occurs over many years, acute events may occur at any time. As these enzymes degrade the fibrous cap within the coronary arteries, unstable plaques rupture and can precipitate thrombi that cause ischemia, leading to acute myocardial infarction [3]. In other vessels, such as the carotid arteries, this sequence of events may lead to ischemic stroke; atherosclerosis in the aorta may cause reduced blood flow to other tissue beds supplied by the systemic vasculature. The development of imaging that can differentiate high-risk, non-flow limiting plaques from others has the potential to alter management of atherosclerosis on a large scale.
Features of high-risk plaques
An expansive body of research has yielded a set of atherosclerotic plaque features that associate with atherothrombosis. While overall plaque burden and high-grade obstruction and ischemia are of clear importance, additional morphological and biological features have more recently been found to independently predict myocardial infarction. Thin-cap fibroatheromas, lesions with a fibrous cap <65 μm in thickness, are thought to be the precursor lesion to most plaque rupture events [4]. Other characteristics of these lesions noted on pathology after cardiac events include necrotic core, macrophage infiltration, mild calcification, and neovascularization [5]. Additionally, bleeding into the necrotic core via the vasa vasorum may predispose to plaque rupture. Moreover, macrophage activity in these lesions is known to be a key driver of atherosclerosis. Inflammatory cells produces metalloproteinases that degrade the structural integrity of the plaque, cause thinning of the fibrous cap and predispose to rupture [6••].
Basis for FDG-PET imaging of inflamed tissues
PET imaging, while most commonly associated with cancer screening and diagnosis, offers a sensitive method to detect tracer uptake in the arterial system. Use of this tracer, 18F-flurodeoxyglucose (FDG), offers the unique opportunity to identify metabolically active tissues [7]. FDG is a glucose analogue that accumulates in cells in proportion to their glycolytic rate. Since inflammatory cells, especially activated ones, have particularly high rates of glycolysis, FDG preferentially enters these cells in order to participate in glycolysis [8, 9]. Furthermore, as macrophages do not store glycogen, they rely on glucose obtained from external sources [10]. However, unlike glucose, once phosphorylated by hexokinase, FDG cannot be metabolized further and is effectively trapped within the cells; its level of accumulation reflects the increased glucose requirement of inflammatory cells [11]. Further, the rate of macrophage glycolysis is proportional to its level of pro-inflammatory activation [12]. Moreover, the relationship between FDG uptake and macrophage density has also been confirmed by histology of tissues [10].
FDG accumulation in tissues can be non-invasively measured by taking advantage of the unique properties of PET. PET imaging uses ionizing radiation in the form of a radiolabeled tracer, most often 18F-labeled radioligands that typically have short half-lives. The injected radiotracers accumulate in tissues after which they release positrons that travel a minute distance then interact with electrons, leading to an annihilation event. At this point, two annihilation photons are created at a 180-degree angle. These photons can then be detected by the PET scanner, which recognizes two photons that arrive simultaneously at 180 degrees apart as having the same tissue origin (thus allowing localization and quantitation) [4, 11]. The distribution of the initial tracer within the body can then be reconstructed digitally.
PET imaging displays greater sensitivity than other imaging modalities, permitting the use of smaller quantities of contrast agents [13]. One disadvantage, however, of nuclear imaging is that the resolution is much less than that of CT or magnetic resonance imaging (MRI). Given that each imaging modality has its own disparate advantages, the combined use of PET/CT or PET/MRI offers the highest quality images in terms of sensitivity and resolution. Unlike PET/CT imaging, PET and MRI imaging sequences may be obtained simultaneously, which may reduce motion artifact and decrease variability between images [11].
Arterial inflammation assessed with PET relates to plaque progression and risk of CVD events
Given the importance of inflammation as a driver of atherosclerosis, PET imaging has been developed and validated as a means to characterize the process. Studies have shown an association between greater FDG PET activity and high-risk plaque morphology in the carotid arteries. In one such study, there was a positive correlation between FDG PET activity and the number of high-risk features present [1, 6••]. Notably, FDG uptake is not simply associated with plaque size; instead, it is the macrophage content of plaques that leads to increased FDG signal [6••, 10]. Furthermore, carotid artery FDG uptake is increased in symptomatic lesions, when compared to asymptomatic lesions [6••]. Similarly, FDG uptake in culprit coronary artery plaques is increased shortly after myocardial infarction [14].
FDG PET measures of arterial inflammation also predict the future behavior of the underlying atheroma. Animal studies have shown that rupture-prone plaques can be identified using FDG PET. In one such study of New Zealand rabbits, plaque rupture was triggered by administration of Russell’s viper venom and histamine. In these rabbits, only plaques with high levels of baseline FDG uptake became atherothrombotic [15]. Similarly, human studies have linked high arterial FDG uptake with subsequent plaque changes. Abdelbaky et al. observed that arterial locations with greater atherosclerotic inflammation later manifest more atherosclerotic plaque progression (as calcium deposition) compared to less-inflamed neighboring locations [16]. Further, short-term changes in arterial inflammation appear to predict future changes in atheroma growth. Joseph et al. recently reported that plaques with short term increases (compared to those with decreases) in inflammation subsequently grow relatively rapidly in size (measured as increased plaque thickness, by MRI) [17].
Moreover, imaging measures of plaque inflammation have been shown to independently predict future clinical events. One study, comprised of patients with known malignancy, suggested that increased FDG uptake may be used to predict cardiovascular events [18]. Another retrospective study showed, in individuals without active cancer, that arterial FDG uptake predicts future cardiovascular disease events and does so beyond clinical risk calculators and calcium scoring [19••]. Moreover, small prospective studies are now demonstrating a similar ability to predict future events. A small study of individuals presenting with acute stroke demonstrated that carotid FDG uptake robustly predicts future stroke risk [20]. Larger prospective studies are needed to better delineate the value of FDG PET imaging for prediction of atherothrombotic risk.
Impact of therapy on PET imaging signal
FDG PET imaging of arterial inflammation has been shown to be highly reproducible in clinically stable patients, with low inter- and intraobserver variability [21]. Such reproducibility offers a potential role for the evaluation of therapeutic interventions. Early animal studies using atherosclerotic animal models demonstrated that lipid lowering agents can lead to a reduction in FDG signal on PET imaging [22]. Likewise, the administration of an atherogenic diet led to increased FDG uptake in the aorta, a signal which could again be reduced after the animals are returned to a normal diet [23]. Human studies have shown similar results. In one such study, simvastatin therapy attenuated plaque uptake of FDG; this effect was not seen with placebo treatment [24]. A multicenter, double-blinded trial that compared the effects of high- versus low-dose atorvastatin showed a dose-effect of statins on the artery wall signal and also demonstrated that anti-inflammatory actions could be seen in individuals already taking low dose statins [25•]. Further, non-pharmacologic lipid-lowering interventions (e.g., apheresis) have also been shown to rapidly reduce the arterial inflammatory signal [26]. Such findings of a beneficial impact of statins are consistent with clinical endpoint trial findings [27].
Further research has shown that concordant imaging and outcomes findings are seen with other drug classes. A 2011 study comparing the effects of pioglitazone to glimepiride on atherosclerotic plaque inflammation in patients with diabetes illustrated attenuation of FDG signal in the carotid arteries and ascending aorta [28]; this finding correlated well with the PROactive study, a 2005 randomized controlled trial that revealed a reduction in macrovascular events among diabetic patients treated with pioglitazone [29]. Conversely, imaging studies that fail to demonstrate a significant change in FDG signal may predict a lack of clinical benefit to therapy, as evidenced by multiple trials involving drugs such as dalcetrapib and darapladib [30–33]. Together, these data suggest that changes in the FDG signal may predict clinical effectiveness of anti-atherosclerotic interventions.
Using PET to derive novel physiologic insights
The scientific value of FDG PET imaging goes beyond the characterization of clinical risk and discovery of novel therapies. Multi-modality imaging with PET can be used to derive important physiologic insights. Disorders of inflammation, including rheumatoid arthritis (RA) and psoriasis or infectious disorders including human immunodeficiency virus (HIV) infection and prosthetic valve endocarditis, have been widely studied using FDG PET/CT imaging.
The most studied of these conditions is HIV infection, which is associated with early atherosclerotic disease and higher risk of cardiac events [34, 35]. Notably, PET imaging displays increased arterial inflammation even in young patients with undetectable levels of viremia and low Framingham Risk Scores [36•]. One recent study showed that FDG signal in lymph nodes can be substantially attenuated in treatment-naïve HIV-infected patients after treatment with antiretroviral therapy [37]. This finding was expected. However, the authors also reported that antiretroviral therapy failed to improve arterial inflammation at all. These findings suggest that efforts to reduce viral activity in HIV may not translate into improvement of arterial inflammation (or the substantial CVD risk seen in HIV).
Though some of the findings of this study were unexpected, they speak to how much research is yet to be done, both in cases of HIV infection and in more generalized disorders of systemic inflammation. FDG PET imaging is likely to be used as a major tool to further this area of discovery (Figs. 1 and 2). Currently, two studies are examining the impact of anti-inflammatory treatments to reduce arterial inflammation in HIV; one investigates the use of low-dose methotrexate (NCT02312219) while the other uses the IL1beta antagonist canakinumab (NCT02272946). It is hoped that insights from trials such as those will one day result in an attenuation of ASCVD risk in HIV-infected individuals.
Limitations of PET imaging
PET imaging allows clinicians and researchers to visualize arterial inflammation and may presage the response to therapeutic interventions. However, despite its many potential uses, it has several notable limitations. Importantly, lesion detection using PET relies inherently on the spatial resolution the PET scanner [38]. Traditional PET scanners have spatial resolution of approximately 4–6 mm, which makes imaging of small coronary arteries difficult. Furthermore, respiratory motion introduces artifact into any image; displacement due to respiratory motion ranges from less than 10 mm to greater than 20 mm depending on the area that is being imaged. Additional limitations of PET imaging have already been described, including the natural uptake of FDG into myocardium surrounding the coronary arteries [39]. Finally, the cost to the patient associated with PET imaging, both financially and in terms of radiation exposure, must be included when assessing the risks and benefits of using imaging as a screening and diagnostic tool.
Novel tracers and next steps
Attempts to reduce the limitations of PET imaging have led to the development of novel tracers as well as technological refinements. Improvements in PET scanners have led to improvements in spatial resolution and more rapid imaging, both of which can modify the effects of respiratory motion artifact [38, 40]. Additional research has focused on the utilization of novel tracers, such as 18-sodium fluoride (18F-NaF). Studies have shown a substantial increase of 18F-NaF at sites of plaque rupture, both within the coronary arteries and within the larger vasculature [41••]. Furthermore, 18F-NaF can potentially differentiate between active and inactive coronary calcification [2••]. Notably, while traditional FDG PET imaging may produce signal in normal or stressed myocytes, myocardial uptake of 18F-NaF is low [2••, 11]. Concurrently, several novel agents targeting inflammation are being explored; these have the potential to provide better specificity for inflammation, along with less myocardial uptake compared to FDG.
Could PET imaging of inflammation be used as a clinical tool?
Improvements in non-invasive imaging for the evaluation of high-risk atherosclerotic plaque could lead to a shift in how ASCVD is treated. In the current era, with new, more expensive drugs being used to treat chronic atherosclerosis (including the PCSK9 antagonists, which cost tens of thousands of dollars per year), there is a growing interest in methods to refine clinical risk assessment. While FDG PET has the potential to provide such enhanced risk prediction, we await the results of prospective trials to better delineate the value of such multi-modality imaging. Ongoing research in this area may provide a clinically useful method by which risk can be stratified appropriately.
Conclusion
Imaging arterial inflammation using FDG PET CT has matured as a scientific tool. The imaging approach is being used to assess novel anti-atherosclerotic therapies and to yield new physiologic insights. Ongoing and future trials are assessing its role in clinical risk assessment.
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Shawnbir Gogia and Yannick Kaiser each declare no potential conflicts of interest.
Ahmed Tawakol reports grants from Actelion, Genetech, and Takeda and personal fees from Actelion, Amgen, Astra Zeneca, and Takeda.
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Gogia, S., Kaiser, Y. & Tawakol, A. Imaging High-Risk Atherosclerotic Plaques with PET. Curr Treat Options Cardio Med 18, 76 (2016). https://doi.org/10.1007/s11936-016-0495-1
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DOI: https://doi.org/10.1007/s11936-016-0495-1