Summary
An efficient, rapid and large-scale propagation of the woody, aromatic and medicinal shrub, Holarrhena antidysenterica, through in vitro culture of nodal segments with axillary buds, is described. N6-benzyladenine used at 15 μM was the most effective in inducing bud break and growth, and also in initiating multiple shoot proliferation at the rate of 43 microshoots per nodal explant with axillary buds, after 30 d of eulture. By repeated subculturing of nodal explants with axillary buds, a high-frequency multiplication rate was established. Efficient rooting was achieved with 35 μM indole-3-butyric acid which was the most effective in inducing roots, as 80% of the microshoots produced roots. Plantlets went through a bardening phase in a controlled plant growth chamber, prior to ex vitro transfer Micropropagated plants established in garden soil were uniform and identical to donor plants with respect to growth characteristics and vegetative morphology.
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Arora, R.; Bhojwani, S. S. In vitro propagation and low temperature storage of Sausurea lappa C.B. Clarke—an endangered medicinal plant. Plant Cell Rep 8:44–47; 1989.
Arya, S.; Sharma, S.; Kaur, R.; Dev Arya, I. Micropropagation of Dendrocalamus asper by shoot proliferation using seeds. Plant Cell Rep. 18:879–882; 1999.
Bera, T. K.; Roy, S. C. Micropropagation of Tylophora indica (Burmn f.) Merr. by multiple bud formation from mature leaf explants without callus intervention. Bot. Bull. Acad. Sin. 34:83–87; 1993.
Bhat, S. R.; Chandal, K. P. S.; Malik, S. R. Plant regeneration from various explants of cultivated Piper species. Plant Cell Rep. 14:395–402; 1995.
Bhojwani, S. S. In vitro propagation of garlic by shoot proliferation. Sci. Hortic. 13:47–52; 1980a.
Bhojwani, S. S. Micropropagation method for a hybrid willow (Salix matsudarna xalba NZ-1002). N.Z.J. Bot. 18:209–214; 1980b.
Bhojwani, S. S. A tissue culture method for a propagation and low temperature storage of Trifolium repens genotypes. Physiol. Plant. 52:187–190; 1981.
Bogetti, B.; Jasik, J.; Mantell, S. In vitro multiplication of cashew (Anacardium occidentale L.) using shoot node explants of glasshouse raised plants. Plant Cell Rep. 18:456–461; 1999.
Cohen, D. Application of micropropagation methods for blueberries and tamarillos. Comb. Proc. Int Plant Prop. Soc. 30:144–146; 1980.
Das, D.K.; Shiva Prakash, N.; Bhalla-Sarin, N. Multiple shoot induction and plant regeneration in litchi (Litchi chinensis Sonn.). Plant Cell Rep. 18:691–695; 1999.
Eeswara, J. P.; Stuchbury, T.; Allan, E. J.; Mordue (Luntz), A. J. A standard procedure for the microprogation of the neem tree (Azadirachta indica A. Juss). Plant Cell Rep. 17:215–219; 1998.
Ghose, S. C. Holarrhena antidysenterica and Wrightia tinctoria. In: Drugs of Hindustan Calcutta Hahnemann Publishing Co. Pvt. Ltd.; 1984:309–320.
Heble, M. R.; Narayanswamy, S.; Chadha, M. S. Metabolism of cholesterol by callus culture of Holarrhena antidysenterica. Phytochemistry 15:1911–1912; 1976.
Kukreja, A. K.; Mathur, A. K.; Zaim, M. Mass production of virus free patchauli plants [Pogostemon cablin (Blanco) Benth] by in vitro culture. Trop. Agric. 67:101–104; 1990.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15:473–497; 1962.
Patnaik, S. K.; Chand, P. K. In vitro propagation of the medicinal herbs Ocimum americanum L. Syn, O. canum Sims (hoary basil) and Ocimum sanctum L. (holy basil). Plant Cell Rep. 15:846–850; 1996.
Purohit, S. D.; Dave, A.; Kukda, G. Micropropagation of safed musli (Chlorophytum boririlianum), a rare medicinal herb. Plant Cell Tiss. Organ Cult, 39:93–96; 1994.
Sahoo, Y.; Chand, P. K. Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high frequency axillary shoot proliferation. Plant Cell Rep. 18:301–307; 1998.
Sen, J.; Sharma, A. K. Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell Tiss. Organ Cult. 26:71–73; 1991.
Sha Valli Khan, P.S.; Prakash, E.; Rao, K. R. In vitro microprogation of an endemic fruit tree Syzygium alternifolium (Wight) walp. Plant Cell Rep. 16:325–328; 1997.
Sharma, N.; Chandel, K. P. S.; Srivastava, V. K. In vitro propagation of Coleus forskohlii Briq., a threatened medicinal plant. Plant Cell Rep. 10:67–70; 1991.
Sudha, G. C.; Seeni, S. In vitro multiplication and field establishment of Adhatoda beddomei CB Clarke, a rare medicinal plant. Plant Cell Rep. 13:203–207; 1994.
Thakur, R.; Rao, P. S.; Bapat, V. A. In vitro plant regeneration in Melia azedarach L. Plant Cell Rep. 18:127–131; 1998.
Wawrosch, C.; Maskay, N.; Kopp, B. Micropropagation of the threatened Nepalese medicinal plant Swertia chirata Buch.-Ham ex. Wall. Plant Cell Rep. 18:997–1001; 1999.
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Raha, S., Chandra Roy, S. In vitro plant regeneration in Holarrhena antidysenterica wall., through high-frequency axillary shoot prolieferation. In Vitro Cell.Dev.Biol.-Plant 37, 232–236 (2001). https://doi.org/10.1007/s11627-001-0041-y
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DOI: https://doi.org/10.1007/s11627-001-0041-y