Abstract
Morphogenetic potential of root, leaf, node and internode expiants of 3 cultivated Piper species was investigated to develop a reliable plant regeneration protocol. P. longum (pipli) was the most responsive followed by P. betle (betel vine) and P. nigrum (black pepper). In P. longum the highest number of shoot buds was produced on root expiants followed by node, internode and leaf expiants. In P. betle and P. nigrum adventitious shoot buds differentiated only from internodal and nodal ring regions, respectively. Histological examination in P. longum showed that adventitious shoot buds originate directly from the cortical cells of the root and the internode without an intervening callus phase. Benzyladenine was superior to kinetin for shoot induction and its optimum concentrations for P. longum, P. betle and P. nigrum were 1–2, 10 and 10 μM, respectively. Shoot elongation and rooting were achieved in B5 medium containing 0.5 μM benzyladenine and 1 μM indoleacetic acid, respectively. Regenerated plants were established in soil.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BA:
-
N6 benzyladenine
- B5 medium:
-
Gamborg et al. (1968) medium
- IAA:
-
indole-3-acetic acid
References
Aminuddin, Johri JK, Anis M, Balasubrahmanyam VR (1993) Curr Sci 65:793–796
Bakshi TS, Coupland RT (1960) Can J Bot 38:243–249
Bhat SR, Chitralekha P, Chandel KPS (1992) Plant Cell Tiss Org Cult 29:19–25
Bhat SR, Kackar A, Chandel KPS (1992) Plant Cell Reports 11:525–528
Fitchet M (1990) Acta Hort 275:285–291
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50:151–158
Mathews VH, Rao PS (1984) Curr Sci 53:183–186
Miller RM, Kaul V, Hutchinson JF, Richards D (1991) Ann Bot 67:35–42
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Philip VJ, Joseph D, Triggs GS, Dickinson NM (1992) Plant Cell Reports 12:41–44
Sharma K, Yeung EC, Thorpe TA (1993) Ann Bot 71:461–466
The Wealth of India (1969) Vol 8, Publications and Information Directorate, CSIR, New Delhi, pp 83–118
Author information
Authors and Affiliations
Additional information
Communicated by G.C. Phillips
Rights and permissions
About this article
Cite this article
Bhat, S.R., Chandel, K.P.S. & Malik, S.K. Plant regeneration from various expiants of cultivated Piper species. Plant Cell Reports 14, 398–402 (1995). https://doi.org/10.1007/BF00238605
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00238605