Abstract
Background and aims
The terrestrial biosphere’s ability to capture carbon is dependent upon soil nitrogen (N) availability, which might reduce as CO2 increases, but global warming has the potential to offset CO2 effects. Here we examine the interactive impact of elevated CO2 (eCO2) and warming on soil N availability and transformations in a low-fertility native grassland in Tasmania, Australia.
Methods
Using ion exchange membranes, we examined soil nitrogen availability during the growing season from 2004 to 2010 in the TasFACE experiment. We also estimated soil N transformation rates using laboratory incubations.
Results
Soil N availability varied strongly over time but was more than doubled by experimental warming of 2°C, an impact that was consistent from the fifth year of the experiment to its conclusion. Elevated CO2 reduced soil N availability by ~28%, although this varied strongly over time. Treatment effects on potential N mineralisation also varied strongly from year to year but tended to be reduced by eCO2 and increased by warming.
Conclusions
These results suggest that warming should increase soil N availability more strongly than it is suppressed by eCO2 in low fertility grasslands such as this, stimulating terrestrial carbon sinks by preventing eCO2-induced nitrogen limitation of primary productivity.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Introduction
Since anthropogenic emissions of CO2 continue to rise, it is critical that we determine whether carbon sink strength will saturate at a particular atmospheric concentration of CO2, as other factors become limiting (Canadell et al. 2008; Pepper et al. 2005). Nitrogen (N) is the mineral nutrient most widely limiting to plant growth and hence to productivity in terrestrial ecosystems (Melillo et al. 1993; Vitousek and Howarth 1991), so that it is often proposed as the primary controller of the biomass response to elevated CO2 concentrations (Finzi et al. 2006; Luo et al. 2004; McMurtrie et al. 2008; Reich et al. 2006b; Ross et al. 1996; Zak et al. 2003). Most N in the terrestrial biosphere is absorbed from the soil as nitrate, ammonium and low molecular weight organic molecules such as amino acids supplied by the decomposition of organic matter (Hofmockel et al. 2011; Hofmockel et al. 2007; Schimel and Bennett 2004). It is evident from both modelling and experimental investigations that N limitation could prevent ecosystem productivity from responding to the strong fertilization effect of rising atmospheric CO2 (Reich and Hobbie 2013; Reich et al. 2006a). Moreover, elevated CO2 (eCO2) alters resource availability to soil microbes (Hu et al. 1999), which might cause a decline in ecosystem N availability through a process termed progressive N limitation (Luo et al. 2004). In essence, the rising CO2 concentration stimulates photosynthesis, increasing plant nutrient demand thereby increasing the proportion of N in the ecosystem that is immobilized in biomass (Luo et al. 2004). Changes in the concentrations of nutrients (Aerts 1997), secondary metabolites (Carrera et al. 2005) or structural components (Moore et al. 1999) in deposited litter could also alter relative rates of mineralization and immobilization. Exudation of C compounds into the soil by plants also increases as CO2 rises (Norby et al. 1987; Uselman et al. 2000), stimulating soil heterotrophs and immobilising N in microbial biomass (de de Graaff et al. 2007), but potentially also increasing microbial mobilisation of N from longer-lived soil organic matter pools (i.e. priming; Bengtson et al. 2012; Drake et al. 2011). It is also possible that root exudations or alteration of soil conditions in response to eCO2 could affect the composition or activity of the soil microbial community (Osanai et al. 2015). These changes in biogeochemistry could feedback on plant protein contents and growth rates (Hu et al. 1999). Over time, therefore, it is possible that the rising CO2 concentration in the atmosphere will actually reduce productivity in terrestrial ecosystems (Hu et al. 1999; Luo et al. 2004; Reich et al. 2006a).
The influence of warming on organic matter decomposition and N mineralization are well-studied (Chung et al. 2013; Gholz et al. 2000; Rustad et al. 2001), but there has been little work examining how increasing temperature will interact with increasing CO2 concentration to influence soil N availability in the field (Dijkstra et al. 2010; Hovenden et al. 2008a; Larsen et al. 2011). The decomposition of organic matter releases organic N into the soil solution as dissolved organic N (DON), which is oxidised to ammonium via ammonification, which is in turn oxidised to nitrate via nitrification, with the net conversion of organic N to inorganic N termed N mineralization. In most ecosystems, increasing the temperature will increase rates of litter decomposition and N mineralization, so it is possible that this response will reduce or even reverse the impacts of rising CO2 concentration on N availability (Parton et al. 2007). The few studies that have specifically examined the interaction between eCO2 and warming on N availability have given varying results such that a meta-analysis by Dieleman et al. (2012) concluded that the positive effects of warming tended to balance the negative effects of eCO2 leading to no net change in N availability in warmer, higher CO2 conditions. This has also been supported by more recent experimental results (Bjorsne et al. 2014). However, major uncertainties relate to the relative time scales of plant and microbial community responses to warming and eCO2 (Reich et al. 2006b; Reich et al. 2004). If these treatment effects resolve over different time-scales then short-term experimental results, even those collected over several years, might not indicate the true magnitude of treatment effects.
A further complication lies in the fact that productivity responses of terrestrial ecosystems to global changes might be co-limited by several different factors. The idea of multiple resource-limitation is that as the availability of any one limiting factor increases, the availability of other factors becomes increasingly limiting to production (Reich and Hobbie 2013; Reich et al. 2014). This interplay between different factors can explain why various systems respond differently or in unpredictable ways to eCO2 but is also one reason that other climate changes might interact with eCO2 in a non-additive manner (Dieleman et al. 2012). For instance, the effects of eCO2 on N-cycling are strongly dependent upon N supply (Reich and Hobbie 2013) but one of the major implications of multiple resource limitation theory is that warming might substantially alter the way that the rising [CO2] will affect ecosystem productivity by changing which factors are limiting and to what degree. Organic matter decomposition rates increase with increasing temperature, so warming will increase the rate of mineralisation of a range of nutrients (Ma et al. 2011), potentially increasing the availability of growth-limiting nutrients and thereby influencing the degree of co-limitation of productivity responses to eCO2. Such information is crucial if terrestrial ecosystem function and C sink strength are to be predicted (Luo et al. 2011; Reich et al. 2006b), so there is a continued need for results from experiments that manipulate other factors in addition to [CO2].
We sought to determine if experimental warming interacted with an increase in CO2 concentration to influence soil N availability and N-mineralisation in plots of native grassland in southeastern Tasmania, Australia and to test whether any treatment-induced changes were sustained over time. In this system, the availability of N is low as are N-fixation rates, therefore there is considerable potential for warming to interact with the eCO2 effect on N availability. Specifically, we test the following hypotheses: 1. the reduction in soil N availability caused by eCO2 is sustained in the longer-term; 2. warming increases soil N availability; and 3. treatment-induced changes in soil N availability are underlain by changes in potential N mineralisation rates.
Methods
Study site and experimental design
The TasFACE Climate Change Facility was established in an area of native lowland temperate grassland on a low fertility basalt plain in south-eastern Tasmania, Australia (42 ° 42′S, 147 ° 16′E). The region has a modified Mediterranean climate characterised by mild moist winters and warm dry summers. Total precipitation is low (~400 mm per annum) and potential evaporation is high (~1250 mm per annum), so there is significant summer drought. The grassland community at the site is species-rich with 51 vascular plant species recorded in the experimental plots. The vegetation is dominated by the perennial grasses Themeda triandra Forssk. (the only C4 species), Rytidosperma caespitosum (Gaudich.) Connor & Edgar and Rytidosperma carphoides (F.Muell. ex Benth.) Connor & Edgar, although almost one-third of the recorded species are native perennial forbs. The full species list is provided elsewhere (Hovenden et al. 2006).
The experiment consisted of 12 Free Air CO2 Enrichment (FACE) rings of 1.5 m diameter, in which vegetation was exposed to either ambient or elevated CO2, and were either warmed or unwarmed. Thus, the experiment was a factorial 2 × 2 design with three replicate plots of each CO2 × warming combination, viz. unwarmed control, warmed control, unwarmed FACE and warmed FACE. FACE rings were fumigated to 550 μmol mol−1 by the FACE method, using a modified pure-CO2 injection system (Hovenden et al. 2006; Miglietta et al. 2001). Warming was provided by the addition of 140 W m−2 of infrared radiation (IR) using 240 V 250 W Emerson Solid Ceramic Infrared Emitters suspended 1.2 m above the soil surface above the centre of each ring. The IR emitters operated continuously and provided an average warming of canopy temperature of 1.98°C and of soil temperature at 1 cm depth by 0.82°C over the year. The warming treatment also reduced relative humidity by an average of 5.1% over the same period. Warming treatment performance was monitored continuously and lamps replaced as they aged to maintain the treatment effects. Full details of experimental design and system performance were provided elsewhere (Hovenden et al. 2006; Hovenden et al. 2008b; Hovenden et al. 2017; Hovenden et al. 2008c). Rainfall and soil moisture conditions are provided in Supplementary Table S1 and Fig. S1.
Soil nutrient analyses
Available soil nitrogen was assessed each spring, the time of maximum plant growth and microbial activity in this ecosystem, using ion exchange membranes as previously described (Hovenden et al. 2008a). As ion exchange membranes interact with the soil solution, they act as artificial roots and provide a measure plant available N rather than the actual soil N concentration (Cain et al. 1999, Bowatte et al. 2007). Dissolved organic N (DON) in the soil consists of both high and low molecular weight fractions, with the low weight fraction consisting predominantly of amino acids (Jones et al. 2005). Since it is only the low weight DON that most plant species can access, this is the organic N pool most important for assessments of overall availability of N in the soil solution. Ion exchange resins compete effectively for low molecular weight DON and therefore provide a useful indication of plant-available organic N (Skogley and Dobermann 1996, Langlois et al. 2003). Ten ion-exchange membrane sticks bearing both an anion and cation exchange resin were deployed per plot each spring from 2004 to 2010. Resin strips were deployed in either late September or early October, with the exact timing depending upon rainfall and temperature patterns but the timing did not vary by more than 2 weeks over the entire 7 year period. Sticks were pushed into the soil until the top of the sheet was at the soil surface, making the effective depth sampled 50 mm; at this site 81 ± 10% of roots occur in this depth region. In each year, sticks were placed in the soil for a 14 day period and replaced immediately with a second set of sticks, meaning that N availability was assessed for the a 4 week period each spring. After removal, membranes were washed with distilled water to remove adhering soil, then the resin membranes were extracted with 25 ml of 0.05 M HCl with shaking for 12 h. Nitrate, ammonium and total soluble N in the resultant extract was measured using an FIAstar 5000 flow injection analyser, (Foss Tecator AB, Hoeganaes, Sweden). Dissolved organic N (DON) was calculated as the difference between total soluble N and mineral N, the sum of ammonium-N and nitrate-N.
Potential net nitrogen mineralization was determined annually using soils sampled from each plot during spring in 2007 to 2010. Four 2.5 cm diameter soil cores 5 cm in depth were collected from each plot at a time corresponding with peak growth. The four soil cores were composited in the field, stored on ice and then returned to the laboratory where soil was sieved to 4 mm and visible roots removed by hand. Initial extractable N was determined by flow injection analysis (as above) following extraction in 100 mL 2 M KCl of a 10 g subsample. A second 10 g sub-sample was incubated at 23°C for 28 d, then final extractable N concentration determined as above. Net ammonification, nitrification and mineralization were calculated as the difference in ammonium, nitrate and mineral N concentrations of pre- and post- incubated soils.
Statistical analysis
Soil available nutrient levels and potential net N mineralisation rates were analysed by a repeated measures two-factor analysis of variance in R (R Development Core Team 2016), with [CO2] and warming the two fixed factors. All data were checked for normality and variance heteroscedasticity and data were transformed where necessary using the Box-Cox transformation in the MASS R package (Venables and Ripley 2002). Where ANOVA indicated there were significant treatment effects, means were compared using Tukey’s Honestly Significant Difference test (Logan 2010).
Results
Nitrogen availability
There were significant impacts of both warming and eCO2 on soil N availability but these impacts tended to vary among years for most measures. Over the 7 year measurement period, total available N (i.e. nitrate + ammonium + low molecular weight organic N) during the period of peak growth was 15.9 ± 2.6 μg N resin−1 fortnight−1 in plots exposed to eCO2 and 21.7 ± 4.5 μg resin−1 fortnight−1 in control plots (F1,8 = 3.49, P = 0.099) but was 139% higher in warmed plots (26.4 ± 4.7 μg resin−1 fortnight−1) than in unwarmed plots (11.3 ± 1.6 μg resin−1 fortnight−1; F1,8 = 22.0, P = 0.002). There was no significant interaction between eCO2 and warming on total available N (F1,8 = 0.11, P = 0.74). The magnitude and significance of these effects, however, was strongly dependent upon time (Fig. 1), with significant Year × Warming (F6,48 = 14.7, P < 0.0001) and Year × CO2 (F6,48 = 3.08, P < 0.01) interactions but no significant three-way interaction (F6,48 = 0.96, P = 0.44). Thus, both the warming and the eCO2 effect on total N availability depended strongly upon year but the treatment effects remained independent (Fig. 1). The variation among years and plots in soil N availability was not related to differences in soil moisture conditions (Fig. S2, S3).
Treatment effects were most pronounced on the availability of nitrate (Fig. 1c) with smaller and less reliable impacts of both warming and eCO2 on the availability of either dissolved organic N (DON; Fig. 1a) or ammonium (Fig. 1b). While both DON and ammonium availability varied over time (F6,48 = 10.1, P < 0.0001 for DON; F6,48 = 10.2, P < 0.0001 for ammonium), there was no general trend in the effect of time nor in the effect of experimental treatments (Fig. 1a, b). Nitrate availability, in contrast, tended to rise substantially from 2004 to 2008, then decline sharply from 2008 to 2010 (Fig. 1c). Since nitrate became the dominant N form in TasFACE soils over this period, total available N followed the same general trajectory (Fig. 1d). Treatment effects on nitrate and total available N similarly became more pronounced over time. Warming increased soil nitrate availability from 2006 to 2008 (Fig. 1c) and total available N from 2007 to 2010 (Fig. 1d).
While eCO2 tended to reduce both nitrate and total N availability, this effect was only significant in 2008 and 2010 for nitrate and in 2008 and 2009 for total N. However, the combination of the generally depressive effects of eCO2 and the significantly stimulatory effects of warming on nitrate and total N availability led to the unwarmed FACE plots having the lowest N availability, followed by the unwarmed control plots with the warmed FACE plots having the second highest N availability and the warmed control plots the highest (Fig. 1d). Treatment effect sizes were unrelated to soil moisture conditions (Fig. S3).
Proportions of soil nitrogen forms
The combined impacts of time and experimental treatments on the availability of individual soil N forms resulted in substantial alterations in the proportion of available N existing in those forms (Fig. 2). While temporal trends dominated the proportion of available N present as DON (F6,48 = 59.9, P < 0.0001), ammonium (F6,48 = 39.9, P < 0.0001) and nitrate (F6,48 = 56.4, P < 0.0001), there were strong, significant and consistent impacts of warming on the proportions of DON (F1,8 = 5.5, P = 0.05; Fig. 2a) and nitrate (F1,8 = 6.0, P = 0.04; Fig. 2c) and a significant year × warming interaction effect on the proportion of ammonium (F6,48 = 3.6, P = 0.005; Fig. 2b). Warming reduced the proportion of DON present in the soil from 34.8 ± 3.9 to 28.8 ± 4.0% but increased the proportion of nitrate present from an overall average of 45.8 ± 4.5 to 55.0 ± 5.0%. Thus, the impact of warming was to accelerate the conversion of organic N to nitrate in soils at TasFACE.
Elevated CO2, in contrast, had no overall impacts on the proportional representation of different N forms but had an impact that tended to vary over time, as evidenced by a significant year × CO2 interaction on the proportion of DON (F1,8 = 2.5, P = 0.03; Fig. 2a) and of nitrate (F1,8 = 3.1, P = 0.01; Fig. 3b). These effects were only expressed in 2009 and 2010, the final years of the experiment (Fig. 2).
Potential nitrogen transformations
In order to determine whether treatments affected the inherent capacity of the TasFACE soils to convert soil organic matter to mineral forms, we assessed N mineralisation rates in TasFACE soils from 2007 to 2010. There was significant variation among years in potential net mineralisation (F1,8 = 23.4, P < 0.0001) as well in potential net ammonification (F1,8 = 4.17, P = 0.009) and net nitrification (F1,8 = 20.9, P < 0.0001). Elevated CO2 tended to reduce both potential nitrification (F1,8 = 6.11, P = 0.04) and net mineralisation (F1,8 = 6.10, P = 0.04), but these effects also varied over time, as evidenced by significant Year × CO2 interactions (F3,72 = 5.59, P = 0.002 for nitrification; F3,72 = 5.22, P = 0.003). There was no overall effect of warming on any of the potential N transformation rates but there was significant Year × Warming interactions for both potential nitrification (F3,72 = 3.44, P = 0.02) and for potential N mineralisation (F3,72 = 4.27, P = 0.008). There were no significant three-way interactions. Therefore, the impact of both eCO2 and warming on potential soil N transformations varied from year to year. Interestingly, experimental treatments influenced potential N transformations significantly in 2008 and 2010 but had no significant effect in either 2007 or 2009 (Fig. 3). However, treatment impacts were similar in 2008 and 2010, with warming increasing potential mineralisation and nitrification while eCO2 substantially reduced both of these N transformations (Fig. 3). In 2009, the trend was similar to in 2008 and 2010 but treatment effects were slight, whereas in 2007 there were no indications of treatment effects. There was little change in ammonium concentration in any treatment in any year, leading to no net ammonification in these soils. Thus, apart from in 2007, the tendency was for warming to accelerate the conversion of organic to mineral N whereas eCO2 reduced the conversion. The large differences in mean net N mineralisation rates among the years were unrelated to differences among years in soil moisture or rainfall patterns (Fig. S4).
Discussion
We had previously suggested, based on the 2004–2006 data, that warming interacted with eCO2 to prevent an eCO2-driven reduction in soil N availability in the TasFACE experiment (Hovenden et al. 2008a). This longer dataset, however, indicates clearly that the warming treatment acted independently of and dominated the CO2 treatment so that plots exposed to both 2°C warming and 550 μmol CO2 mol−1 were not just the same as the controls (as in the early years) but had consistently higher nitrogen availability. This difference was maintained from year 6 (i.e. 2007) to the conclusion of the TasFACE experiment after nine full years of treatments (i.e. 2010). These results differ substantially from earlier research which mostly suggested that modest experimental warming tended to negate but not overwhelm the effects of eCO2 on soil N availability (Bjorsne et al. 2014; Dieleman et al. 2012; Hovenden et al. 2008a) or that the influence of warming was insufficient to offset the eCO2 effect (Dijkstra et al. 2012). Instead, we found that the impact of eCO2 was smaller and more variable than the warming effect, agreeing with results from the BioCON that indicated that eCO2 had smaller and less consistent impacts on soil N pools and fluxes than other factors (Mueller et al. 2013). This is unlikely to be due to the TasFACE ecosystem being unusually responsive to warming, as the acceleration of N mineralisation by warming was well within the range of previous studies (Rustad et al. 2001). While the results from the PHACE experiment in semi-arid rangeland, the experiment most similar to TasFACE, showed that eCO2 and warming effects on soil N varied substantially from year to year (Carrillo et al. 2012), there is evidence that warming can also overwhelm the eCO2-induced reduction in soil nitrate availability at least in some years (Mueller et al. 2016), supporting our results. However, treatment effects in the PHACE experiment were related to soil moisture conditions, with greater impacts occurring in drier years (Mueller et al. 2016), which was not the case at TasFACE (Fig. S3).
Importantly, we found no real indication of any sustained interaction between eCO2 and warming on either N availability or N mineralisation rates. Thus, it is unlikely that elevated CO2 increased N availability when N mineralisation was stimulated by warming, as had been suggested elsewhere (Chen et al. 2016; Pastore et al. 2016). In the TasFACE experiment warming and eCO2 effects on N mineralisation and availability were essentially additive and independent, meaning that the response to the combined treatment could be predicted from the response to either applied in isolation. Unlike the situation in other systems (Chen et al. 2016; Hasegawa et al. 2016; Ross et al. 2013; Rütting and Andresen 2015), we found no evidence that eCO2 increased N availability or mineralisation in any year, aligning with the situation in the PHACE experiment (Carrillo et al. 2012; Mueller et al. 2016). While variation among replicate plots led to few years in which eCO2 significantly affected N availability, it was clear that soils in unwarmed FACE plots had the lowest nitrate and total available N concentrations of the four treatment combinations (Fig. 1). The strong variation among years in potential N mineralisation rates is surprising but might indicate a dynamic and variable soil microbial community responding to large interannual variation in rainfall. In the years when treatment effects on N mineralisation did occur, they were consistent and had the same impacts observed in field N availability. Thus, soil from warmed plots had higher potential N mineralisation than soil from unwarmed plots and that from FACE plots had lower potential mineralisation rates than occurred in soil from control plots (Fig. 3), with no evidence of interactions between the two. Further, our estimates of potential N mineralisation were done at a single temperature under standardised laboratory conditions. Hence, the realised N mineralisation rate in the warmed plots is likely to be higher than that observed in the incubations, since the soil temperature in the experiment is higher in warmed plots. Therefore, the in situ estimates of N availability and the laboratory estimations of the soil’s ability to convert organic N to mineral forms both indicated that eCO2 would reduce N availability while warming would increase it. The biggest differences were observed in the production and availability of nitrate. Experiments in forest (Bader et al. 2013) and woodland (Hungate et al. 2014) have demonstrated increased leaching of nitrate in response to eCO2, which could be caused by changes in hydrology, soil N-transformations or a combination of the two. Our results indicate that only changes in hydrology would lead to increased nitrate leaching in this system, since nitrate production or availability was not increased by eCO2. Our results do not support an eCO2-derived increase in N availability, rather supporting the reduction in N:P observed in the PHACE experiment (Dijkstra et al. 2012). However, unlike the situation at PHACE, the combination of warming and eCO2 in the TasFACE experiment increased N:P via warming-derived changes in N availability (Ma et al. 2011). This is important, because it indicates that a modest increase in temperature, only 2°C in the canopy and 1°C in the soil, could be sufficient to prevent eCO2-driven reductions in N availability in this system, as also occurred in the PHACE experiment in 2011 and 2012 (Mueller et al. 2016). Importantly, variation among years in the impact of both warming and eCO2 on soil N availability have been linked to variation in soil moisture conditions (Carrillo et al. 2012; Mueller et al. 2016; Osanai et al. 2017), but this does not appear to be the case at TasFACE, in which the soil N availability and treatment impacts were not related to soil moisture availability (Fig. S2, S3).
The reductions in N availability in response to eCO2 that occurred in the TasFACE experiment are unlikely to have been driven by altered immobilisation of N into plant biomass and therefore the mechanism believed to be the main driver of progressive N limitation, i.e. eCO2-induced growth stimulation (Luo et al. 2004), is probably not the primary cause of the reduction in N availability in the FACE plots. Biomass production at TasFACE was only stimulated by eCO2 in 2006 and 2007 (Hovenden et al. 2014) and was unaffected in the other years during the study period. Certainly, eCO2 did not lead to an increase in the amount of N sequestered in plant biomass in this experiment (Hovenden et al. 2014). Had eCO2 led to a more consistent stimulation of growth at TasFACE, N availability might have been reduced further and more consistently and in such a situation warming might not have increased N availability in eCO2 plots to as great an extent. This situation, then, would be more akin to that observed in the PHACE experiment (Dijkstra et al. 2010), in which eCO2 had a greater impact than warming on N availability. The increases in N availability in the warmed plots are also unlikely to be due to reductions in plant demand for N, since biomass production was largely unaffected by warming in the TasFACE experiment (data not shown). Therefore, we believe that the treatment-induced alterations in N availability in this experiment were not driven by changes in plant uptake.
This finding is consistent with the explanation for PNL in grassland described in Newton et al. (2010). Unlike forests that have a woody biomass pool providing long-term N storage that encourages PNL to occur, grassland plants are often ephemeral and long-term N storage is in the soil rather than the plant. This means that PNL is driven by soil processes that themselves are frequently dependent on microbial activity and consequently PNL effects have the potential to vary spatially and temporally.
In the TasFACE experiment, the reduction in soil N was likely a result of reduced rates of N mineralisation. Both warming and eCO2 altered the C-to-N ratio of above and below ground material at TasFACE (Osanai et al. 2015; Pendall et al. 2011), with the changes corresponding to the differences in N mineralisation rates observed here. This, coupled with treatment-induced alterations of the soil microbial community composition and function (Hayden et al. 2012; Osanai et al. 2015) are the most likely drivers of the alterations in N availability at TasFACE. Since changes to litter quality (Franck et al. 1997; Gahrooee 1998; Hattenschwiler et al. 1999; Hirschel et al. 1997; Kasurinen et al. 2007; Kemp et al. 1994) and microbial community function (Carney et al. 2007; Drigo et al. 2008; Ebersberger et al. 2004; Hayden et al. 2012; Osanai et al. 2015; Pinay et al. 2007) are widespread in global change experiments, we contend that these changes alone are likely to drive future alterations to nutrient cycling in grasslands, with potential consequences for ecosystem productivity.
Our results also demonstrate that it takes several years before treatment effects on biogeochemical processes are realised, possibly because changes in the quality of organic matter entering the soil will take some time to alter the overall nature of the soil organic matter pool unless inputs are great in comparison to the existing pool size. The reasons that warming effects were not evident during the first four to 5 years of the experiment remain uncertain but a threshold might have been reached after 5 or 6 years of cumulative additions of lower C-to-N tissues that induced a shift from immobilization to mineralization in the warmed plots. It is also likely that treatment effects on the soil environment play a role in the delay. Since potential N mineralisation in the warmed FACE plots was essentially the same as in the unwarmed control plots, due to similarity in litter C-to-N ratio, the differences in soil N availability in the field are likely to be due to treatment effects on the environment, potentially on both biotic and abiotic components. The warming treatment increased soil temperature by approximately 1°C (Hovenden et al. 2006), which would partially account for the increased N availability as warming accelerates litter decomposition (van van Meeteren et al. 2008). However, experimental warming also reduces soil moisture content, particularly during the wet winter and spring months (Hovenden et al. 2008b), which would increase aeration thereby increasing nitrification and reducing denitrification, which is an anaerobic process and most likely to occur during moist periods (Bollmann and Conrad 1998). In an American prairie, warming reduced soil moisture and productivity also after a substantial lag period (Sherry et al. 2008). The treatments at TasFACE were also likely to influence plant behaviour, especially root exudation, altered levels of which would have influenced microbial activity and immobilization/mineralization rates (Bais et al. 2006; Bardgett et al. 1999; Broeckling et al. 2008; Kuzyakov et al. 2007; Phillips et al. 2009), thereby contributing to differences between field N availability and laboratory mineralization rates.
These results demonstrate that eCO2 can lead to a reduction in soil N availability without the need for increased sequestration of N into plant biomass but that warming of just 2°C at the soil surface overwhelms this reduction. Therefore, N availability in systems in which the stimulation of biomass by eCO2 is slight might actually have increased N availability in the future, driven by acceleration of N mineralisation rates. Since the acceleration of decomposition and therefore nutrient cycling is likely to be tightly dependent upon the degree of warming, future nutrient availability and therefore ecosystem productivity will be highly dependent upon just how much warming accompanies the rise in CO2 concentration.
References
Aerts R (1997) Climate, leaf litter chemistry and leaf litter decomposition in terrestrial ecosystems: a triangular relationship. Oikos 79:439–449
Bader MKF, Leuzinger S, Keel SG, Siegwolf RTW, Hagedorn F, Schleppi P, Korner C (2013) Central European hardwood trees in a high-CO2 future: synthesis of an 8-year forest canopy CO2 enrichment project. J Ecol 101:1509–1519. https://doi.org/10.1111/1365-2745.12149
Bais HP, Weir TL, Perry LG, Gilroy S, Vivanco JM (2006) The role of root exudates in rhizosphere interactions with plants and other organisms. Annu Rev Plant Biol 57:233–266
Bardgett RD, Mawdsley JL, Edwards S, Hobbs PJ, Rodwell JS, Davies WJ (1999) Plant species and nitrogen effects on soil biological properties of temperate upland grasslands. Funct Ecol 13:650–660
Bengtson P, Barker J, Grayston SJ (2012) Evidence of a strong coupling between root exudation, C and N availability, and stimulated SOM decomposition caused by rhizosphere priming effects. Ecol Evol 2:1843–1852. https://doi.org/10.1002/ece3.311
Bjorsne A-K, Rutting T, Ambus P (2014) Combined climate factors alleviate changes in gross soil nitrogen dynamics in heathlands. Biogeochemistry 120:191–201. https://doi.org/10.1007/s10533-014-9990-1
Bollmann A, Conrad R (1998) Influence of O-2 availability on NO and N2O release by nitrification and denitrification in soils. Glob Chang Biol 4:387–396
Broeckling CD, Broz AK, Bergelson J, Manter DK, Vivanco JM (2008) Root exudates regulate soil fungal community composition and diversity. Appl Environ Microbiol 74:738–744. https://doi.org/10.1128/AEM.02188-07
Bowatte S, Asakawa S, Okada M, Kobayashi K, Kimura M (2007) Effect of elevated atmospheric CO concentration on ammonia oxidizing bacteria communities inhabiting in rice roots. Soil Sci Plant Nutr 53 (1):32–39
Cain ML, Subler S, Evans JP, Fortin MJ (1999) Sampling spatial and temporal variation in soil nitrogen availability. Oecologia 118 (4):397–404
Canadell JG, Pataki DE, Gifford RM, Houghton RA, Luo Y, Raupach MR, Smith P, Steffen W (2008) Saturation of the terrestrial carbon sink. In: Canadell JG, Pataki DE, Pitelka LF (eds) Terrestrial ecosystems in a changing world. Springer-Verlag, Berlin
Carney KM, Hungate BA, Drake BG, Megonigal JP (2007) Altered soil microbial community at elevated CO2 leads to loss of soil carbon. Proc Natl Acad Sci U S A 104:4990–4995
Carrera AL, Vargas DN, Campanella MV, Bertiller MB, Sain CL, Mazzarino MJ (2005) Soil nitrogen in relation to quality and decomposability of plant litter in the Patagonian Monte, Argentina. Plant Ecol 181:139–151
Carrillo Y, Dijkstra FA, Pendall E, Morgan JA, Blumenthal DM (2012) Controls over soil nitrogen pools in a semiarid grassland under elevated CO2 and warming. Ecosystems 15:761–774. https://doi.org/10.1007/s10021-012-9544-0
Chen ZZ, Zhang JB, Xiong ZQ, Pan GX, Muller C (2016) Enhanced gross nitrogen transformation rates and nitrogen supply in paddy field under elevated atmospheric carbon dioxide and temperature. Soil Biol Biochem 94:80–87. https://doi.org/10.1016/j.soilbio.2015.11.025
Chung H, Muraoka H, Nakamura M, Han S, Muller O, Son Y (2013) Experimental warming studies on tree species and forest ecosystems: a literature review. J Plant Res 126:447–460. https://doi.org/10.1007/s10265-013-0565-3
de Graaff MA, Six J, van Kessel C (2007) Elevated CO2 increases nitrogen rhizodeposition and microbial immobilization of root-derived nitrogen. New Phytol 173:778–786
Development Core Team R (2016) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna
Dieleman WIJ, Vicca S, Dijkstra FA, Hagedorn F, Hovenden MJ, Larsen KS, Morgan JA, Volder A, Beier C, Dukes JS, King J, Leuzinger S, Linder S, Luo Y, Oren R, de Angelis P, Tingey D, Hoosbeek MR, Janssens IA (2012) Simple additive effects are rare: a quantitative review of plant biomass and soil process responses to combined manipulations of CO2 and temperature. Glob Chang Biol 18:2681–2693. https://doi.org/10.1111/j.1365-2486.2012.02745.x
Dijkstra FA, Blumenthal D, Morgan JA, Pendall E, Carrillo Y, Follett RF (2010) Contrasting effects of elevated CO2 and warming on nitrogen cycling in a semiarid grassland. New Phytol 187:426–437. https://doi.org/10.1111/j.1469-8137.2010.03293.x
Dijkstra FA, Pendall E, Morgan JA, Blumenthal DM, Carrillo Y, LeCain DR, Follett RF, Williams DG (2012) Climate change alters stoichiometry of phosphorus and nitrogen in a semiarid grassland. New Phytol 196:807–815. https://doi.org/10.1111/j.1469-8137.2012.04349.x
Drake JE, Gallet-Budynek A, Hofmockel KS, Bernhardt ES, Billings SA, Jackson RB, Johnsen KS, Lichter J, McCarthy HR, McCormack ML, Moore DJP, Oren R, Palmroth S, Phillips RP, Pippen JS, Pritchard SG, Treseder KK, Schlesinger WH, DeLucia EH, Finzi AC (2011) Increases in the flux of carbon belowground stimulate nitrogen uptake and sustain the long-term enhancement of forest productivity under elevated CO2. Ecol Lett 14:349–357. https://doi.org/10.1111/j.1461-0248.2011.01593.x
Drigo B, Kowalchuk GA, van Veen JA (2008) Climate change goes underground: effects of elevated atmospheric CO2 on microbial community structure and activities in the rhizosphere. Biol Fertil Soils 44:667–679
Ebersberger D, Werrnbter N, Niklaus PA, Kandeler E (2004) Effects of long term CO2 enrichment on microbial community structure in calcareous grassland. Plant Soil 264:313–323
Finzi AC, Moore DJP, DeLucia EH, Lichter J, Hofmockel KS, Jackson RB, Kim HS, Matamala R, McCarthy HR, Oren R, Pippen JS, Schlesinger WH (2006) Progressive nitrogen limitation of ecosystem processes under elevated CO2 in a warm-temperate forest. Ecology 87:15–25
Franck VM, Hungate BA, Chapin FS, Field CB (1997) Decomposition of litter produced under elevated CO2: dependence on plant species and nutrient supply. Biogeochemistry 36:223–237
Gahrooee FR (1998) Impacts of elevated atmospheric CO2 on litter quality, litter decomposability and nitrogen turnover rate of two oak species in a Mediterranean forest ecosystem. Glob Chang Biol 4:667–677
Gholz HL, Wedin DA, Smitherman SM, Harmon ME, Parton WJ (2000) Long-term dynamics of pine and hardwood litter in contrasting environments: toward a global model of decomposition. Glob Chang Biol 6:751–765
Hasegawa S, Macdonald CA, Power SA (2016) Elevated carbon dioxide increases soil nitrogen and phosphorus availability in a phosphorus-limited eucalyptus woodland. Glob Chang Biol 22:1628–1643. https://doi.org/10.1111/gcb.13147
Hattenschwiler S, Buhler S, Korner C (1999) Quality, decomposition and isopod consumption of tree litter produced under elevated CO2. Oikos 85:271–281
Hayden HL, Mele PM, Bougoure DS, Allan CY, Norng S, Piceno YM, Brodie EL, DeSantis TZ, Andersen GL, Williams AL, Hovenden MJ (2012) Changes in the microbial community structure of bacteria, archaea and fungi in response to elevated CO2 and warming in an Australian native grassland soil. Environ Microbiol 14:3081–3096. https://doi.org/10.1111/j.1462-2920.2012.02855.x
Hirschel G, Körner C, Arnone JA (1997) Will rising atmospheric CO2 affect leaf litter quality and in situ decomposition rates in native plant communities? Oecologia 110:387–392
Hofmockel KS, Schlesinger WH, Jackson RB (2007) Effects of elevated atmospheric carbon dioxide on amino acid and NH4+−N cycling in a temperate pine ecosystem. Glob Chang Biol 13:1950–1959. https://doi.org/10.1111/j.1365-2486.2007.01411.x
Hofmockel KS, Gallet-Budynek A, McCarthy HR, Currie WS, Jackson RB, Finzi AC (2011) Sources of increased N uptake in forest trees growing under elevated CO2: results of a large-scale N-15 study. Glob Chang Biol 17:3338–3350. https://doi.org/10.1111/j.1365-2486.2011.02465.x
Hovenden MJ, Miglietta F, Zaldei A, Vander Schoor JK, Wills KE, Newton PCD (2006) The TasFACE climate-change impacts experiment: design and performance of combined elevated CO2 and temperature enhancement in a native Tasmanian grassland. Aust J Bot 54:1–10
Hovenden MJ, Newton PCD, Carran RA, Theobald P, Wills KE, Schoor JKV, Williams AL, Osanai Y (2008a) Warming prevents the elevated CO2-induced reduction in available soil nitrogen in a temperate, perennial grassland. Glob Chang Biol 14:1018–1024. https://doi.org/10.1111/j.1365-2486.2008.01558
Hovenden MJ, Newton PCD, Wills KE, Janes JK, Williams AL, Vander Schoor JK, Nolan MJ (2008b) Influence of warming on soil water potential controls seedling mortality in perennial but not annual species in a temperate grassland. New Phytol 180:143–152
Hovenden MJ, Wills KE, Chaplin RE, Vander Schoor JK, Williams AL, Osanai Y, Newton PCD (2008c) Warming and elevated CO2 affect the relationship between seed mass, germinability and seedling growth in Austrodanthonia caespitosa, a dominant Australian grass. Glob Chang Biol 14:1633–1641
Hovenden MJ, Newton PCD, Wills KE (2014) Seasonal not annual rainfall determines grassland biomass response to carbon dioxide. Nature 511:583–586. https://doi.org/10.1038/nature13281
Hovenden MJ, Porter M, Newton PCD (2017) Elevated CO2 and warming effects on grassland plant mortality are determined by the timing of rainfall. Ann Bot 119:1225–1233. https://doi.org/10.1093/aob/mcx006
Hu S, Firestone MK, Chapin IIIFS (1999) Soil microbial feedbacks to atmospheric CO2 enrichment. Trends Ecol Evol 14:433–437
Hungate BA, Duval BD, Dijkstra P, Johnson DW, Ketterer ME, Stiling P, Cheng W, Millman J, Hartley A, Stover DB (2014) Nitrogen inputs and losses in response to chronic CO2 exposure in a subtropical oak woodland. Biogeosciences 11:3323–3337. https://doi.org/10.5194/bg-11-3323-2014
Jones DL, Healey JR, Willett VB, Farrar JF, Hodge A (2005) Dissolved organic nitrogen uptake by plants - an important N uptake pathway? Soil Biol Biochem 37:413–423
Kasurinen A, Peltonen PA, Julkunen-Tiitto R, Vapaavuori E, Nuutinen V, Holopainen T, Holopainen JK (2007) Effects of elevated CO2 and O-3 on leaf litter phenolics and subsequent performance of litter-feeding soil macrofauna. Plant Soil 292:25–43
Kemp PR, Waldecker DG, Owensby CE, Reynolds JF, Virginia RA (1994) Effects of elevated CO2 and nitrogen-fertilization pretreatments on decomposition on tallgrass prairie leaf-litter. Plant Soil 165:115–127
Kuzyakov Y, Hill PW, Jones DL (2007) Root exudate components change litter decomposition in a simulated rhizosphere depending on temperature. Plant Soil 290:293–305
Langlois JL, Johnson DW, Mehuys GR (2003) Adsorption and recovery of dissolved organic phosphorus and nitrogen by mixed-bed ion-exchange resin. Soil Sci Soc Am J 67:889–894
Larsen KS, Andresen LC, Beier C, Jonasson S, Albert KR, Ambus PER, Arndal MF, Carter MS, Christensen S, Holmstrup M, Ibrom A, Kongstad J, van der Linden L, Maraldo K, Michelsen A, Mikkelsen TN, Pilegaard KIM, PriemÉ A, Ro-Poulsen H, Schmidt IK, Selsted MB, Stevnbak K (2011) Reduced N cycling in response to elevated CO2, warming, and drought in a Danish heathland: synthesizing results of the CLIMAITE project after two years of treatments. Glob Chang Biol 17:1884–1899. https://doi.org/10.1111/j.1365-2486.2010.02351.x
Logan M (2010) Biostatistical design and analysis using R: a practical guide. Wiley-Blackwell, Chichester
Luo Y, Su B, Currie WS, Dukes JS, Finzi A, Hartwig U, Hungate B, McMurtrie RE, Oren R, Parton WJ, Pataki DE, Shaw MR, Zak DR, Field CB (2004) Progressive nitrogen limitation of ecosystem responses to rising atmospheric carbon dioxide. Bioscience 54:731–739
Luo Y, Melillo J, Niu S, Beier C, Clark JS, Classen AT, Davidson E, Dukes JS, Evans RD, Field CB, Czimczik CI, Keller M, Kimball BA, Kueppers LM, Norby RJ, Pelini SL, Pendall E, Rastetter E, Six J, Smith M, Tjoelker MG, Torn MS (2011) Coordinated approaches to quantify long-term ecosystem dynamics in response to global change. Glob Chang Biol 17:843–854. https://doi.org/10.1111/j.1365-2486.2010.02265.x
Ma LN, Lü XT, Liu Y, Guo JX, Zhang NY, Yang JQ, Wang RZ, Bond-Lamberty B (2011) The Effects of Warming and Nitrogen Addition on Soil Nitrogen Cycling in a Temperate Grassland, Northeastern China. PLoS ONE 6 (11):e27645
McMurtrie RE, Norby RJ, Medlyn BE, Dewar RC, Pepper DA, Reich PB, Barton CVM (2008) Why is plant-growth response to elevated CO2 amplified when water is limiting, but reduced when nitrogen is limiting? A growth-optimisation hypothesis. Funct Plant Biol 35:521–534
Melillo JM, McGuire AD, Kicklighter DW, Moore B, Vorosmarty CJ, Schloss AL (1993) Global climate-change and terrestrial net primary production. Nature 363:234–240. https://doi.org/10.1038/363234a0
Miglietta F, Peressotti A, Primo Vacari F, Zaldei A, De Angelis P, Scarscia Mugnozza G (2001) Free air CO2 enrichment (FACE) of a poplar plantation: the POPFACE fumigation system. New Phytol 150:465–476
Moore TR, Trofymow JA, Taylor B, Prescott C, Camiré C, Duschene L, Fyles J, Kozak L, Kranabetter M, Morrison I, Siltanen M, Smith S, Titus B, Visser S, Wein R, Zoltai S (1999) Litter decomposition rates in Canadian forests. Glob Chang Biol 5:75–82
Mueller KE, Hobbie SE, Tilman D, Reich PB (2013) Effects of plant diversity, N fertilization, and elevated carbon dioxide on grassland soil N cycling in a long-term experiment. Glob Chang Biol 19:1249–1261. https://doi.org/10.1111/gcb.12096
Mueller KE, Blumenthal DM, Pendall E, Carrillo Y, Dijkstra FA, Williams DG, Follett RF, Morgan JA (2016) Impacts of warming and elevated CO2 on a semi-arid grassland are non-additive, shift with precipitation, and reverse over time. Ecol Lett 19:956–966. https://doi.org/10.1111/ele.12634
Newton PCD, Lieffering M, Bowatte WMSD, Brock SC, Hunt CL, Theobald PW, Ross DJ, (2010) The rate of progression and stability of progressive nitrogen limitation at elevated atmospheric CO2 in a grazed grassland over 11Â years of Free Air CO2 enrichment. Plant and Soil 336 (1–2):433–441
Norby RJ, O'Neill EG, Hood WG, Luxmore RJ (1987) Carbon allocation, root exudation and mycorrhizal colonization of Pinus echinata seedlings grown under CO2 enrichment. Tree Physiol 3:203–210
Osanai Y, Janes JK, Newton PCD, Hovenden MJ (2015) Warming and elevated CO2 combine to increase microbial mineralisation of soil organic matter. Soil Biol Biochem 85:110–118. https://doi.org/10.1016/j.soilbio.2015.02.032
Osanai Y, Tissue DT, Bange MP, Braunack MV, Anderson IC, Singh BK (2017) Interactive effects of elevated CO2, temperature and extreme weather events on soil nitrogen and cotton productivity indicate increased variability of cotton production under future climate regimes. Agric Ecosyst Environ 246:343–353. https://doi.org/10.1016/j.agee.2017.06.004
Parton WJ, Morgan JA, Wang GM, del Grosso S (2007) Projected ecosystem impact of the prairie heating and CO2 enrichment experiment. New Phytol 174:823–834. https://doi.org/10.1111/j.1469-8137.2007.02052.x
Pastore MA, Megonigal JP, Langley JA (2016) Elevated CO2 promotes long-term nitrogen accumulation only in combination with nitrogen addition. Glob Chang Biol 22:391–403. https://doi.org/10.1111/gcb.13112
Pendall E, Osanai Y, Williams AL, Hovenden MJ (2011) Soil carbon storage under simulated climate change is mediated by plant functional type. Glob Chang Biol 17:505–514. https://doi.org/10.1111/j.1365-2486.2010.02296.x
Pepper DA, Del Grosso SJ, McMurtrie RE, Parton WJ (2005) Simulated carbon sink response of shortgrass steppe, tallgrass prairie and forest ecosystems to rising [CO2], temperature and nitrogen input. Glob Biogeochem Cycles 19:GB1004
Phillips RP, Bernhardt ES, Schlesinger WH (2009) Elevated CO2 increases root exudation from loblolly pine (Pinus Taeda) seedlings as an N-mediated response. Tree Physiol 29:1513–1523. https://doi.org/10.1093/treephys/tpp083
Pinay G, Barbera P, Carreras-Palou A, Fromin N, Sonie L, Couteaux MM, Roy J, Philippot L, Lensi R (2007) Impact of atmospheric CO2 and plant life forms on soil microbial activities. Soil Biol Biochem 39:33–42
Reich PB, Hobbie SE (2013) Decade-long soil nitrogen constraint on the CO2 fertilization of plant biomass. Nat Clim Chang 3:278–282. https://doi.org/10.1038/nclimate1694
Reich PB, Tilman D, Naeem S, Ellsworth DS, Knops J, Craine J, Wedin D, Trost J (2004) Species and functional group diversity independently influence biomass accumulation and its response to CO2 and N. Proc Natl Acad Sci U S A 101:10101–10106
Reich PB, Hobbie SE, Lee T, Ellsworth DS, West JB, Tilman D, Knops JMH, Naeem S, Trost J (2006a) Nitrogen limitation constrains sustainability of ecosystem response to CO2. Nature 440:922
Reich PB, Hungate BA, Luo YQ (2006b) Carbon-nitrogen interactions in terrestrial ecosystems in response to rising atmospheric carbon dioxide. Annu Rev Ecol Evol Syst 37:611–636
Reich PB, Hobbie SE, Lee TD (2014) Plant growth enhancement by elevated CO2 eliminated by joint water and nitrogen limitation. Nat Geosci 7:920–924. https://doi.org/10.1038/ngeo2284
Ross DJ, Saggar S, Tate KR, Feltham CW, Newton PCD (1996) Elevated CO2 effects on carbon and nitrogen cycling in grass/clover turves of a Psammaquent soil. Plant Soil 182:185–198
Ross DJ, Newton PCD, Tate KR, Luo D (2013) Impact of a low level of CO2 enrichment on soil carbon and nitrogen pools and mineralization rates over ten years in a seasonally dry, grazed pasture. Soil Biol Biochem 58:265–274. https://doi.org/10.1016/j.soilbio.2012.12.011
Rustad LE, Campbell JL, Marion GM, Norby RJ, Mitchell MJ, Hartley AE, Cornelissen JHC, Gurevitch J (2001) A meta-analysis of the response of soil respiration, net nitrogen mineralization, and aboveground plant growth to experimental ecosystem warming. Oecologia 126:543–562
Rütting T, Andresen L (2015) Nitrogen cycle responses to elevated CO2 depend on ecosystem nutrient status. Nutr Cycl Agroecosyst 101:285–294. https://doi.org/10.1007/s10705-015-9683-8
Schimel JP, Bennett J (2004) Nitrogen mineralization: challenges of a changing paradigm. Ecology 85:591–602
Sherry RA, Weng ES, Arnone JA, Johnson DW, Schimel DS, Verburg PS, Wallace LL, Luo YQ (2008) Lagged effects of experimental warming and doubled precipitation on annual and seasonal aboveground biomass production in a tallgrass prairie. Glob Chang Biol 14:2923–2936. https://doi.org/10.1111/j.1365-2486.2008.01703.x
Skogley EO, Dobermann A, (1996) Synthetic Ion-Exchange Resins: Soil and Environmental Studies. J Environ Qual. 25 (1):13
Uselman SM, Qualls RG, Thomas RB (2000) Effects of increased atmospheric CO2, temperature and soil N availability on root exudation of dissolved organic carbon by a N-fixing tree (Robinia pseudoacacia L.) Plant Soil 284:319–333
van Meeteren MM, Tietema A, van Loon EE, Verstraten JM (2008) Microbial dynamics and litter decomposition under a changed climate in a Dutch heathland. Appl Soil Ecol 38:119–127. https://doi.org/10.1016/j.apsoil.2007.09.006
Venables WN, Ripley BD (2002) Modern applied statistics with S. Springer, New York
Vitousek PM, Howarth RW (1991) Nitrogen limitation on land and in the sea - how can it occur. Biogeochemistry 13:87–115
Zak DR, Holmes WE, Finzi AC, Norby RJ, Schlesinger WH (2003) Soil nitrogen cycling under elevated CO2: a synthesis of forest face experiments. Ecol Appl 13:1508–1514
Acknowledgements
This research was funded through the Australian Research Council’s Discovery Projects grant scheme. Amity Williams and Jasmine Janes assisted with data collection and Phil Theobald helped with N determinations. We thank the Australian Department of Defence for access to the Pontville Small Arms Range complex. The authors confirm no conflict of interests.
Author information
Authors and Affiliations
Corresponding author
Additional information
Responsible Editor: Zucong Cai
Electronic supplementary material
ESM 1
(DOCX 167 kb)
Rights and permissions
About this article
Cite this article
Hovenden, M.J., Newton, P.C.D. & Osanai, Y. Warming has a larger and more persistent effect than elevated CO2 on growing season soil nitrogen availability in a species-rich grassland. Plant Soil 421, 417–428 (2017). https://doi.org/10.1007/s11104-017-3474-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11104-017-3474-8