Abstract
As the main form of land use and human disturbance of grassland, livestock grazing has great influences on the soil resources and plant communities. This study observed the variation of soil properties and community characteristics of four treatments of different grazing intensity (no grazing, UG; light grazing, LG; moderate grazing, MG; and heavy grazing, HG) in an alpine meadow of Sichuan Province on the northeastern margin of the Tibetan Plateau. The results showed that grazing increased the pH, soil bulk density (BD), and contents of total carbon (TC) and total nitrogen (TN), and the BD increased while the others decreased with the grazing intensity. At the community level, with the increase of the grazing intensity, the vegetation coverage (R 2 = 0.61, P < 0.001), mean height of community (R 2 = 0.37, P < 0.001), aboveground biomass (R 2 = 0.54, P < 0.001), litter biomass (R 2 = 0.84, P < 0.001), and percentage of aboveground biomass of palatable grasses to total biomass (R 2 = 0.74, P < 0.001) significantly decreased, while the belowground biomass (R 2 = 0.72, P < 0.001) and the root/shoot (R/S) ratio (R 2 = 0.65, P < 0.001) increased. The species richness was the greatest at LG and the total biomass at UG. With grazing, the dominant species of the plant community shifted from palatable grasses (Gramineae and Cyperaceae) to unpalatable grasses (Compositae and Ranunculaceae). Based on the results, LG may be the optimal grassland management mode to be used in the long time in the alpine meadow of the Tibetan Plateau.
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Introduction
Nearly 25% of the world’s land surface is occupied by grassland (Scurlock and Hall 1998), which was used in ways such as grazing (Luo et al. 2009; Zhou et al. 2006), enclosure (Ma et al. 2015), reclamation (Santoso et al. 1997), and other modern management (e.g., use of fertilizers, reseeding) (Clare et al. 2004). As its main form of land use (Luo et al. 2009) and human disturbance (Zhou et al. 2006), livestock grazing has a great influence on the soil resources and plant community of grassland (Deng et al. 2014b). Grazing has been found to influence the physical, chemical, and biological properties of soil (Altesor et al. 2006; Steffens et al. 2008; Zhu et al. 2016; Su et al. 2004). The direct result of livestock trampling includes destruction of soil structure (Su et al. 2005; Deng et al. 2014b) and reduction of infiltration rates and porosity of soil (Wu et al. 2010). The soil nutrient cycling and storage are affected through nutrient input and release (Cui et al. 2005), such as urine and feces directly inputted in the system (Bai et al. 2012; An and Li 2015). In addition, grazing also affects the microbial structure, enzyme activities (Su et al. 2004), and microbial activities (An and Li 2015) in the soil. The grassland community is found to be changed by grazing (Altesor et al. 2006) through selective defoliation, trampling, and excreta deposition (Wrage et al. 2012; Jerrentrup et al. 2015). Such change is mainly in terms of its structure and function (Altesor et al. 2005; Luo et al. 2012). Structurally, grazing modifies the species composition, richness, vertical profiles, plant traits, and a number of other attributes of grasslands. And functionally, grazing alters the flow of energy and the cycling of materials (Altesor et al. 2005; Pineiro et al. 2006). Abundant evidence demonstrates that the bite effect of grazing livestock reduces the vegetation coverage and the height of community (Medina-Roldán et al. 2012; Zhu et al. 2016). The influence of grazing on the plant community lies in two ways, i.e., the direct impact through diet preference and trampling of livestock on plant’s survival, growth, and fecundity and the indirect one by altering the intraspecific and interspecific interactions or the water and nutrient availability (Wan et al. 2010). The response of the aboveground and belowground biomass allocation to grazing, however, is unclear, because the strategies of allocation in plant under grazing are very controversial (Yang et al. 2009; Gao et al. 2007; An and Li 2015), which led to observation results of decreased belowground biomass (Deng et al. 2014a) and also increased ones (Gao et al. 2007) related to grazing.
Compared with grazing exclusion, slight and moderate grazing is generally believed to be beneficial for biodiversity and aboveground biomass production of grassland ecosystems (Cui et al. 2005). However, overgrazing is one of main causes of grassland desertification (Deng et al. 2014a; Su et al. 2005; Wu et al. 2009), including desertification of soil properties (chemical and physical) and negative effects of vegetation, including reduction in biomass, vegetation coverage, and species diversity and increase in undesirable vegetation (Deng et al. 2014a; Zhu et al. 2016). Grazing prohibition (exclusion) or fencing is the common grassland management to reverse desertification grassland throughout the world in recent decades (Deng et al. 2014b; Deng et al. 2014a; Ma et al. 2015; Wu et al. 2009), and Feng et al. (2010) founded that Elymus natans establishment is an effective and applicable measure in restoring heavily degraded alpine meadow in the region of the Qinghai-Tibetan Plateau through 3-and 7-year-old E. natans re-vegetated grasslands.
As the third pole of the Earth (average elevation 4000 m a.s.l.), the Tibetan Plateau is the largest plateau in the world covering an area of about 2.5 million km2 in China (Chen et al. 2014; Zhang et al. 2016). The dominant vegetation type in this region is the alpine meadow which occupies more than 40% of grassland on the plateau and provides fundamental sources of livelihood for local residents and ecosystem services (Zhang et al. 2016). However, the alpine meadow has been deteriorating recently, resulting in serious social and ecological problems (Zhang et al. 2016; Zhou et al. 2006). Previous studies of different grazing intensities showed that increased grazing intensity tends to increase biomass allocation of belowground and a potential to increase the ecosystem pool of plant N (Gao et al. 2007), that long-time grazing may cause the degradation of alpine meadow, and that moderate grazing may be desirable for plant communities in the Tibetan Plateau (Zhou et al. 2006). Currently, 4.67 × 106 hm2 alpine meadow is degraded, accounting for 25% of the total area of this region and approximately half of the total alpine grassland (Dong et al. 2013). In terms of vegetation recovery and soil property improvement for the alpine meadow, livestock exclusion or fencing rather than grazing was found to be beneficial, which changes the biomass and soil properties to the better (Shi et al. 2010; Ma et al. 2015; Wu et al. 2009). The lightly and moderately degraded alpine meadow was found to recover by being kept away from disturbance, whereas the heavily and extremely heavily degraded alpine meadow requires artificial and semi-artificial grassland establishment for ecosystem restoration (Dong et al. 2013; Feng et al. 2010).
Recent research of alpine meadow in the Tibetan Plateau has focused on the effects of grazing vegetation (coverage, height, biomass, species richness, species diversity, species composition, etc.) or soil properties (physical, chemical, and biological) (Dong et al. 2013; Gao et al. 2007; Ma et al. 2015; Shi et al. 2010; Zhou et al. 2006). Few studies explored the effects of grazing intensity both on soil and plant through the community shift. With the hypothesis that optimal grazing is beneficial for both soil properties and vegetation of alpine meadow in the Tibetan Plateau, this study aimed to (i) evaluate the effects of the grazing intensity on soil dynamics and (ii) analyze the community characteristics and community composition with different grazing intensities in the Tibetan Plateau. Based on the results, the variation of soil properties, community characteristics, and community composition of four different grazing intensity treatments would be assessed and the optimal management of alpine meadow in the Tibetan Plateau could be drawn for better maintenance and improvement of soil properties and the plant community.
Materials and methods
Study site
The study area is located in an alpine meadow in Hongyuan County, in the northwest region of Sichuan Province and on the northeastern margin of the Tibetan Plateau, China (34°54′ N, 102°06′ E, and 3480 m a.s.l.) (Fig. 1). For the past three decades, its mean annual temperature was 0.9 °C, with mean monthly temperature ranging from −10.3 °C (January) to 10.9 °C (July), and the precipitation fluctuates greatly among years, averaging about 690 mm each year, with 80% occurring during the growing season (May to August) (Shi et al. 2015). The area is characterized by alpine meadow soil (Chinese classification). The typical alpine meadow is dominated by Gramineae (Elymus nutans Griseb., Deschampsia caespitosa (L.) Beauv., Festuca ovina L., Koeleria cristata (L.) Pers.), Cyperaceae (Carex enervis C. A. Mey., Scirpus distigmaticus (Kukenth.) Tang et Wang), Compositae (Anaphalis lactea Maxim., Saussurea nigrescens Maxim., Leontopodium leontopodioides (Willd.) Beauv.) and Ranunculaceae (Ranunculus tanguticus (Maxim.) Ovcz., Anemone trullifolia Hook. f. et Thoms. var. linearis (Brühl) Hand.-Mazz, and Anemone rivularis Buch.-Ham. ex DC. var. flore-minore Maxim.) species. The vegetation has a mean coverage of 95% and a height of 10 cm.
Experimental design
We designed four different grazing intensity treatments (no grazing, UG; light grazing, LG; moderate grazing, MG; and heavy grazing, HG), based on factors including acreage, aboveground biomass, theoretical intakes of yak, and grazing time. The area of UG was 1.6 hm2, that of LG 4.2 hm2, that of MG 2.5 hm2, and that of HG 1.9 hm2; the grazing intensity was 0, 0.7, 1.2, and 1.6 yaks hm−2, respectively (Table 1). The yaks grazed in the corresponding experiment plots from May to October 2010–2015.
Each treatment area except for UG had three replicates; each replicate included three plots of 1 m × 1 m at least 20 m away from each other. For UG, nine plots (1 m × 1 m) at least 10 m from each other were randomly selected, every three plots being regarded as a replicate. Altogether, the experiment included 36 plots of 1 m × 1 m, which were all set in July 2015 when the aboveground biomass peaked. The plants in every plot were identified by morphology and later taxonomy. The vegetation coverage was measured by visually recording the grids and the height of 5–10 individuals of each species in each plot.
Biomass and importance value computation
For each plot, quadrates of 50 cm × 50 cm were randomly selected to investigate the aboveground biomass and the litter of the community, which were defined as the green part and all the other parts of the aboveground, respectively. For the same quadrates, belowground biomass was taken from 0- to 30-cm depths (with 90% of roots) by a soil core (4 cm in diameter). The aboveground and belowground parts as well as the litter were dried to constant weight at 60 °C. The belowground biomass (BB) was calculated as
where DR is the dry weight of roots in each soil core and d is 4.
The percentage of aboveground biomass of palatable grasses to total biomass (PPG) was calculated as
where PPG is the aboveground biomass of Gramineae and Cyperaceae species and TAB is the total biomass of all species in the quadrate (Wu et al. 2009).
The root shoot ratio (R/S) was calculated as
where BB is the belowground biomass, AB is the aboveground biomass, and LB is the litter biomass.
The importance value (IV) per family was calculated as
where RH is the relative height (RH = height of one family / total height of all vegetation), RC is the relative coverage (RC = coverage of one family / total coverage of all vegetation), and RB is the relative aboveground biomass (RB = aboveground biomass of one family / total aboveground biomass of all vegetation).
Soil sampling and determination
For each plot, two soil samples were taken from 0 to 10 cm depth by a soil core (4 cm in diameter). The fresh soil samples were sieved through a 2-mm mesh and air dried. Then, the air-dried samples were divided into two parts, one passed through a 0.25-mm mesh for pH and the other through a 0.15-mm mesh for determination of total carbon (TC), total nitrogen (TN), and total phosphorus (TP).
After the soil was dried at 105 °C, the bulk density (BD, g cm−3) was measured by a soil bulk sampler (5 cm in diameter and 5 cm in height) as Mora and Lázaro (2014) described. Soil pH was measured by an acidity meter (Sartorius PB-10, Gemany), with the ratio of air-dried soil and water as 1:5 (Lee et al. 2012). Soil TC content was determined by the Elementar Liqui II TOC (Elementar, Germany). Soil TN and TP contents were determined by the Kjeldahl method with an Kjeldahl analyzer (Kjeltec 8400, FOSS, Sweden) and colorimetrically after digestion with H2SO4 and HClO4 by the molybdenum blue method (Li et al. 2013). The contents of TC, TN, and TP were converted into content in per unit soil dry weight.
Statistical analysis
All statistical (soil pH, bulk density, total carbon, total nitrogen, total phosphorus, coverage, mean height of community, species richness, aboveground, litter, belowground and total biomass, percentage of aboveground biomass of palatable grasses to total aboveground biomass, root/shoot ratio, importance value of each family, family number, and species number) tests were performed with StatGraphics 3.0 (STN, St. Louis, MO, USA) and analyzed by one-way (grazing intensity) ANOVA after the data were tested for normality. All P values of these multi-comparisons were corrected by the Tukey HSD test. All data were expressed as mean ± standard error (SE). Differences between means were considered significant when the P value of the ANOVA F test was less than 0.05.
Results
Variation in soil physicochemical properties
The pH, content of TC and TN was lower in the UG than in the grazing meadow and decreased with the increase of grazing intensity (Fig. 2a, 2c and 2d). No significant difference of pH (Fig. 2a), BD (Fig. 2b), content of TC (Fig. 2c), TN (Fig. 2d) and TP (Fig. 2e) was found among treatments of different grazing intensity. The BD was significantly higher in treatments of greater grazing intensity (P < 0.05, Fig. 2b, Table S1). The soil TP content was nearly equal among all treatments (between 1.08 and 1.15 g kg−1) (Fig. 2e). Correlation analysis indicated a significant negative correlation between TC and BD (R 2 = 0.303, P = 0.037) and a significant positive correlation between TC and TN (R 2 = 0.356, P = 0.024) (Table 2).
Variation in community characteristics
At the plant community level, the vegetation coverage (Fig. 3a) and the mean height of community (Fig. 3b) decreased significantly with the grazing intensity (P < 0.001, Table 3). Species richness decreased with the order of LG > UG > MG > HG (Fig. 3c). No significant difference of species richness (Table 3) was found among the grazing intensities.
At the plant population level, species composition was found to be quite different among the four grazing intensities, with the most species in LG (57.67) and the least species (50) in MG and HG (Table 4). The number of family was the same in UG and MG (18.67), the highest in LG (19), and the lowest in HG (18.33) (Table 4). At the plant family level, Compositae had the highest importance value in all grazing intensities (22.77% in LG, 21.60% in MG, and 24.99% in HG) except for UG where Gramineae had the greatest importance value of 27.67% (Table 4). The summed importance value of Gramineae and Cyperaceae decreased with the grazing intensity (43.01% in UG, 31.74% in LG, 28.71% in MG, and 28.77% in HG), while the summed importance value of Compositae and Ranunculaceae increased with the grazing intensity (24.08% in UG, 33.83% in LG, 38.77% in MG, and 37.17% in HG) (Table 4). With the increase of grazing intensity, the dominant species of the plant community shifted from Gramineae and Cyperaceae to Compositae and Ranunculaceae (Table 4).
Variation in biomass and root shoot ratio
At the plant community level, with the increase of the grazing intensity, the aboveground biomass (Fig. 4a), litter biomass (Fig. 4b), and percentage of aboveground biomass of palatable grasses to total biomass (Fig. 4e) all decreased, while the belowground biomass (Fig. 4c), total biomass (Fig. 4d), and R/S ratios (Fig. 4f) increased significantly (P < 0.05).
Correlation analyses indicated that the grazing intensity was significantly and positively correlated to the aboveground biomass, litter biomass, and percentage of aboveground biomass of palatable grasses (Table 3). A significantly negative linear correlation was observed between grazing intensity and the belowground biomass, total biomass, and R/S ratio (Table 3).
Discussion
The influence of grazing on the soil physicochemical properties
Generally, the direct influence of grazing on the grassland soil is litter trampling, vegetation coverage reduction, and feces and urine deposition (Ma et al. 2015). Previous studies revealed higher pH and BD in the grazing soil than in the no-grazing one in desert steppe (Deng et al. 2014a), hill landscape (Deng et al. 2014b), degraded sandy grassland (Su et al. 2005), and alpine meadow (Ma et al. 2015; Wu et al. 2010). Soil pH is one of the main factors determining grassland plant community composition and is mainly determined by parent material and organic and inorganic matter content (Basto et al. 2015). The variation in pH in this study is probably related to vegetation coverage, variations of root systems, and organic matter content (Su et al. 2005). The BD is an important factor affecting key soil functions including soil water, nutrients, and carbon stocks (Mora and Lázaro 2014) and therefore is an indicator of soil porosity versus compaction (Yang et al. 2015). Our experiment showed significantly increased BD in plots of higher grazing intensities (Fig. 2b, Table S1). Such variation tendency in BD could arise for two reasons. First, the increased grazing intensity could lead to soil compaction and could reduce soil infiltration rates and soil porosity, creating higher BD through animal hooves (Wu et al. 2010; Murphy et al. 2004). Second, the increase in grazing intensity could reduce the plant coverage (Fig. 3a) which in turn, by removed topsoil and exposed denser subsoil, would increase the potential of compaction and wind and rain erosion (Murphy et al. 2004).
Different from other study results that grazing could decrease TC and TN contents of soil (Deng et al. 2014b; Su et al. 2005; Ma et al. 2015), our experiment found lower TC and TN contents in UG than in treatments of grazing meadow, with a significant positive correlation between TC and TN contents (P < 0.05, Table 2). Since grazing can affect soil chemical properties by altering the soil nutrient cycle (Fernández-Lugo et al. 2013), the following mechanisms could attribute to the different trends observed between our study and others. First, organic matter is the main reservoir of C and N in terrestrial ecosystems (Golluscio et al. 2009), and in this study the total biomass of the grazing area was more than (nearly equal) that in the UG area (Fig. 3d), probably because grazing stimulated grass investing more resources in roots (Fig. 3b). Second, grazing increases plant biomass turnover through consumption (Medina-Roldán et al. 2012). Additionally, grazing often increases C-rich root exudates that stimulate microbial activity and turnover (Bai et al. 2012), consequently increasing TC and TN contents in the grazed sites and yielding a significant positive correlation between them. However, the reduction in TC and TN concentrations with the grazing intensity in our study could also be ascribed to the following reasons. First, the soil organic matter (SOM) is determined by main inputs from decomposition of aboveground and belowground vegetation litter and animal excreta and main outputs though the mineralization and leaching of carbon and nitrogen (Golluscio et al. 2009; Cui et al. 2005). Second, grazing markedly reduced vegetation coverage (Fig. 3a), thereby accelerating decomposition and erosion (Ma et al. 2015). Additionally, the soil BD increased with the grazing intensity (Fig. 2b), which could reduce the microbial activity and decrease the quality and decomposition rate of the litter. On the other hand, feces and urine of yaks on the soil surface are an input of organic matter into soils, which may stimulate microbial biomass and activity leading to net C and N mineralization (Ma et al. 2015). Consequently, the TC and TN contents decreased as the grazing intensity increased.
Unlike variation of TC and TN contents, the concentration of TP was nearly the same in the treatments of all grazing intensities (Fig. 2e). This was probably because the main source of P is rock weathering, which is a long process (Shen et al. 2011) and not significantly influenced by grazing. A negative correlation between TC and BD (P = 0.037, Table 2) was found in this study, like some others (Steffens et al. 2008; Yang et al. 2015). This could be due to the fact that BD depends on the size and density of soil mineral and organic particles and how they are packed together (Mora and Lázaro 2014); therefore, low BD is a remarkable feature of soil organic matter immobilization (Yang et al. 2015).
The influence of grazing on community characteristics
Our study showed the highest number of species and families in the LG, which was in agreement with our hypothesis that optimal grazing is beneficial for both soil properties and vegetation of alpine meadow in the Tibetan Plateau. Similarly, Weithoff et al. (2001) also found high mean diversity with disturbances of intermediate intensity. To adapt to the variations of the environment, plant is often modified in different environments. As in the former research (Deng et al. 2014a), our results suggested significant decreases in the vegetation coverage (Fig. 3a) and the mean height of community (Fig. 3b) with the increase in grazing intensity (yaks hm−2) (P < 0.001, Table 3), probably resulting from the bite effect of grazing livestock (Medina-Roldán et al. 2012; Zhu et al. 2016).
Species richness is sensitive to environmental heterogeneity (Pykälä 2003). Previous research suggested that grazing exclusion increases species richness (Deng et al. 2014a; Fensham et al. 2011). In agreement with former studies (Fensham et al. 2011; Deng et al. 2014a), our results showed a significant unimodal relation between the grazing intensity and the species richness (Fig. 3c) and number of species and families (Table 4), respectively, with the species richness and species number being the highest in the LG. However, the effects of grazing vary from area to area, mainly depending on the grazing intensity and history (Hu et al. 2015). Our results showed no significant difference in the correlation of species richness with grazing intensity in all treatments (Fig. 3c, Table 3), which was similar to the previous study in the Tibetan Plateau (Zhou et al. 2006). It suggested that grazing intensity may not be an important factor influencing species richness (Deng et al. 2014a). Former research considered the duration of grazing exclusion an important factor affecting the species richness in the Tibetan Plateau (Wu et al. 2012).
At the community level, the plant community composition was observed to be altered with grazing or fencing (grazing exclusion) (Wu et al. 2009), which was characterized by the variations of the ratio between palatable grasses and unpalatable grasses. With grazing, the dominant species of the plant community shifted from palatable grasses (Gramineae and Cyperaceae) in UG to unpalatable grasses (Compositae and Ranunculaceae) in grazed meadow (Table 4). Herbivory is one of the important factors that strongly influence competitive relationships between plant species (Borgström et al. 2016). The change of dominant species between un-grazed and grazed meadow plots was probably due to the specific diet preference of the yaks in our experiment. Through their diet preference, the yaks reduced the proportion of palatable grasses in the species and the biomass in the whole community and therefore made dominant the unpalatable grasses. From the perspective of the grasses, palatable grasses have greater competitive ability than unpalatable grasses in the absence of grazing (Moretto and Distel 1997; Wu et al. 2009) and more regenerative potency under the disturbance of grazing (Belsky et al. 1992). In addition, the proportion of palatable grasses of the community is the largest in lightly or moderately grazed grassland, but the vigor of palatable grasses is reduced in overgrazed ones. However, the unpalatable grasses usually have “escape and defense strategies” to avoid the damage of grazing (Belsky et al. 1992).
The influence of grazing on biomass and root to shoot ratio
The biomass allocation of plant organs (e.g., root, stem, leaf) reflects the plant ecological adaptation to changes of environmental changes including grazing disturbance. In our study, the aboveground biomass (Fig. 4a), litter biomass (Fig. 4b) and percentage of aboveground biomass of palatable grasses to total biomass (Fig. 4e) were lower in the grazed treatments than in the ungrazed area and had a significant and positive linear correlation with the grazing intensity, respectively (Table 3). Opposite results were found in belowground biomass (Fig. 4c, Table 3) and total biomass (Fig. 4d, Table 3). The results about aboveground and litter biomass were similar with previous findings (Deng et al. 2014a; Gao et al. 2007). This tendency should be a direct result of the consumption of biomass by the grazers (Gao et al. 2007; An and Li 2015). Grazing affects the root biomass by trampling and excretions, but the root biomass responses to livestock grazing are ambiguous (Gao et al. 2007; An and Li 2015). Our results indicated that the belowground biomass tended to increase with the grazing intensity (Fig. 4c) and had a negative linear correlation with the grazing intensity (Table 3). This was probably because plants growing in heavily grazed areas invested more resources in roots than those in less grazed or ungrazed ones (Gao et al. 2007). And the increasing biomass allocation to the roots is an important adaptive response of plants to grazing (An and Li 2015).
Aboveground and belowground biomass are different forms to fulfill the plant function as a whole (Mokany et al. 2006), which make aboveground and belowground biomass allocation a central issue in plant ecology (Yang et al. 2009). Former researchers used the R/S ratio to gain a greater understanding of the relationship between aboveground and belowground biomass. The R/S ratio is an index for the differential investment of plant photosynthates between the aboveground and belowground, a complicated process which is the cumulative response of vegetation to biotic, abiotic, and management influences (Mokany et al. 2006). In order to adapt to the changing environment conditions, the R/S ratio of plants is often modified to maximize the ability to capture resources (Deng et al. 2014a). We found that the R/S ratio significantly increased with grazing intensity (Fig. 4f, Table 3), indicating that grazing changed the aboveground and belowground biomass allocation (Fig. 4a and 4e). Such result was supported by the optimal partitioning hypothesis (Bai et al. 2012). When grazing intensity is increased, plants invest more resources to support root growth. Former studies indicated that a high proportion of root biomass in the total biomass can enhance the capacity to tolerate environmental stresses and external disturbances (An and Li 2015; Gao et al. 2007).
Conclusion
The present study suggests that compared to the UG, grazing increased the pH, soil bulk density, and content of TC and TN, where BD increased with grazing intensity at the grazed plots, and the opposite was true for the pH and contents of TC and TN. In addition, we found that with the increased grazing intensity, the coverage, mean height of community, aboveground biomass, litter biomass and percentage of aboveground biomass of palatable grasses to total biomass significantly decreased, while the belowground biomass and R/S ratio increased. Furthermore, the dominant species of the plant community shifted from palatable grasses (Gramineae and Cyperaceae) to unpalatable ones (Compositae and Ranunculaceae). Our results highlight that LG is the optimal grassland management mode in this region, because of the greatest species richness and relatively higher aboveground biomass under this management.
References
Altesor, A., Oesterheld, M., Leoni, E., Lezama, F., & Rodríguez, C. (2005). Effect of grazing on community structure and productivity of a Uruguayan grassland. Plant Ecology, 179(1), 83–91.
Altesor, A., Piñeiro, G., Lezama, F., Jackson, R. B., Sarasola, M., & Paruelo, J. M. (2006). Ecosystem changes associated with grazing in subhumid South American grasslands. Journal of Vegetation Science, 17(3), 323–332.
An, H., & Li, G. Q. (2015). Effects of grazing on carbon and nitrogen in plants and soils in a semiarid desert grassland, China. Journal of Arid Land, 7(3), 341–349.
Bai, Y. F., Wu, J. G., Clark, C. M., Pan, Q. M., Zhang, L. X., Chen, S. P., Wang, Q. B., & Han, X. G. (2012). Grazing alters ecosystem functioning and C:N:P stoichiometry of grasslands along a regional precipitation gradient. Journal of Applied Ecology, 49(6), 1204–1215.
Basto, S., Thompson, K., & Rees, M. (2015). The effect of soil pH on persistence of seeds of grassland species in soil. Plant Ecology, 216(8), 1163–1175.
Belsky, A. J., Carson, W. P., Jensen, C. L., & Fox, G. A. (1992). Overcompensation by plants: herbivore optimization or red herring? Evolutionary Ecology, 7(1), 109–121.
Borgström, P., Strengbom, J., Viketoft, M., & Bommarco, R. (2016). Aboveground insect herbivory increases plant competitive asymmetry, while belowground herbivory mitigates the effect. Peer J, 4.
Chen, H., Yang, G., Peng, C. H., Zhang, Y., Zhu, D., Zhu, Q. A., Hu, J., Wang, M., Zhan, W., Zhu, E. X., Bai, Z. Z., Li, W., Wu, N., Wang, Y. F., Gao, Y. H., Tian, J. Q., Kang, X. M., Zhao, X. Q., & Wu, J. H. (2014). The carbon stock of alpine peatlands on the Qinghai–Tibetan Plateau during the Holocene and their future fate. Quaternary Science Reviews, 95(7), 151–158.
Clare, S. L., Martin, A. F., & Mitchley, J. (2004). The influence of seed addition and cutting regime on the success of grassland restoration on former arable land. Applied Vegetation Science, 7(2), 259–266.
Cui, X. Y., Wang, Y. F., Niu, H. S., Wu, J., Wang, S. P., Schnug, E., Rogasik, J., Fleckenstein, J., & Tang, Y. H. (2005). Effect of long-term grazing on soil organic carbon content in semiarid steppes in Inner Mongolia. Ecological Research, 20(5), 519–527.
Deng, L., Sweeney, S., & Shangguan, Z. P. (2014a). Grassland responses to grazing disturbance: plant diversity changes with grazing intensity in a desert steppe. Grass and Forage Science, 69(69), 524–533.
Deng, L., Zhang, Z. N., & Shangguan, Z. P. (2014b). Long-term fencing effects on plant diversity and soil properties in China. Soil and Tillage Research, 137(1), 7–15.
Dong, Q. M., Zhao, X. Q., Wu, G. L., Shi, J. J., & Ren, G. H. (2013). A review of formation mechanism and restoration measures of “black-soil-type” degraded grassland in the Qinghai-Tibetan Plateau. Environmental Earth Sciences, 70(5), 2359–2370.
Feng, R. Z., Long, R. J., Shang, Z. Z., Ma, Y. S., Dong, S. K., & Wang, Y. L. (2010). Establishment of Elymus natans improves soil quality of a heavily degraded alpine meadow in Qinghai-Tibetan Plateau, China. Plant and Soil, 327(1–2), 403–411.
Fensham, R. J., Silcock, J. L., & Dwyer, J. M. (2011). Plant species richness responses to grazing protection and degradation history in a low productivity landscape. Journal of Vegetation Science, 22(6), 997–1008.
Fernández-Lugo, S., Bermejo, L. A., Nascimento, L. D., Méndez, J., Naranjo-Cigala, A., & Arévalo, J. R. (2013). Productivity: key factor affecting grazing exclusion effects on vegetation and soil. Plant Ecology, 214(4), 641–656.
Gao, Y. H., Luo, P., Wu, N., Yi, S. L., & Chen, H. (2007). Biomass and nitrogen responses to grazing intensity in an alpine meadow on the eastern Tibetan Plateau. Polish Journal of Ecology, 55(3), 469–479.
Golluscio, R. A., Austin, A. T., Martínez, G. C. G., Gonzalez-Polo, M., Sala, O. E., & Jackson, R. B. (2009). Sheep grazing decreases organic carbon and nitrogen pools in the Patagonian steppe: combination of direct and indirect effects. Ecosystems, 12(4), 686–697.
Hu, F. L., Liu, B., Liu, Z. M., Fang, Y. T., & Busso, C. A. (2015). Effects of grazing on plant species diversity and carbon partitioning in semiarid rangelands of northeastern China. Phyton, 84, 209–221.
Jerrentrup, J. S., Seither, M., Petersen, U., & Isselstein, J. (2015). Little grazer species effect on the vegetation in a rotational grazing system. Agriculture, Ecosystems and Environment, 202, 243–250.
Lee, H., Schuur, E. A. G., Inglett, K. S., Lavoie, M., & Chanton, J. P. (2012). The rate of permafrost carbon release under aerobic and anaerobic conditions and its potential effects on climate. Global Change Biology, 18(2), 515–527.
Li, H., Li, J., He, Y. L., Li, S. J., Liang, Z. S., Peng, C. H., Polle, A., & Luo, Z. B. (2013). Changes in carbon, nutrients and stoichiometric relations under different soil depths, plant tissues and ages in black locust plantations. Acta Physiologiae Plantarum, 35(10), 2951–2964.
Luo, C. Y., Xu, G. P., Chao, Z. G., Wang, S. P., Lin, X. W., Hu, Y. G., Zhang, Z. H., Duan, J. C., Chang, X. F., Su, A. L., Li, Y. N., Zhao, X. Q., Du, M. Y., Tang, Y. H., & Kimball, B. (2009). Effect of warming and grazing on litter mass loss and temperature sensitivity of litter and dung mass loss on the Tibetan plateau. Global Change Biology, 16(5), 1606–1617.
Luo, G. P., Han, Q. F., Zhou, D. C., Li, L., Chen, X., Li, Y., Hu, Y. K., & Li, B. L. (2012). Moderate grazing can promote aboveground primary production of grassland under water stress. Ecological Complexity, 11, 126–136.
Ma, W. M., Ding, K. Y., & Li, Z. W. (2015). Comparison of soil carbon and nitrogen stocks at grazing-excluded and yak grazed alpine meadow sites in Qinghai-Tibetan Plateau, China. Ecological Engineering, 87, 203–211.
Medina-Roldán, E., Paz-Ferreiro, J., & Bardgett, R. D. (2012). Grazing exclusion affects soil and plant communities, but has no impact on soil carbon storage in an upland grassland. Agriculture Ecosystems and Environment, 149(149), 118–123.
Mokany, K., Raison, R. J., & Prokushkin, A. S. (2006). Critical analysis of root: shoot ratios in terrestrial biomes. Global Change Biology, 12(1), 84–96.
Mora, J. L., & Lázaro, R. (2014). Seasonal changes in bulk density under semiarid patchy vegetation: the soil beats. Geoderma, 235–236, 30–38.
Moretto, A. S., & Distel, R. A. (1997). Competitive interactions between palatable and unpalatable grasses native to temperate semi-arid grassland of Argentina. Plant Ecology, 130(2), 155–161.
Murphy, C. A., Foster, B. L., Ramspott, M. E., & Price, K. P. (2004). Grassland management effects on soil bulk density. Transactions of the Kansas Academy of Science, 107(1), 45–54.
Pineiro, G., Paruelo, J., & Oesterheld, M. (2006). Potential long-term impacts of livestock introduction on carbon and nitrogen cycling in grasslands of Southern South America. Global Change Biology, 12(7), 1267–1284.
Pykälä, J. (2003). Effects of restoration with cattle grazing on plant species composition and richness of semi-natural grasslands. Biodiversity and Conservation, 12(11), 2211–2226.
Santoso, D., Adiningsih, S., Mutert, E., Fairhurst, T., Noordwijk, M. V., & Garrity, D. P. (1997). Soil fertility management for reclamation of Imperata grasslands by smallholder agroforestry. Agroforestry Systems, 36(1), 181–202.
Scurlock, J. M. O., & Hall, D. O. (1998). The global carbon sink: a grassland perspective. Global Change Biology, 4(2), 229–233.
Shen, J. B., Yuan, L. X., Zhang, J. L., Li, H. G., Bai, Z. H., Chen, X. P., Zhang, W. F., & Zhang, F. S. (2011). Phosphorus dynamics: from soil to plant. Plant Physiology, 156(3), 997–1005.
Shi, F. S., Chen, H., Wu, Y., & Wu, N. (2010). Effects of livestock exclusion on vegetation and soil properties under two topographic habitats in an alpine meadow on the eastern Qinghai-Tibetan Plateau. Polish Journal of Ecology, 58(1), 125–133.
Shi, C. G., Silva, L. C. R., Zhang, H. X., Zheng, Q. Y., Xiao, B. X., Wu, N., & Su, G. (2015). Climate warming alters nitrogen dynamics and total non-structural carbohydrate accumulations of perennial herbs of distinctive functional groups during the plant senescence in autumn in an alpine meadow of the Tibetan Plateau, China. Agricultural and Forest Meteorology, 200, 21–29.
Steffens, M., Kölbl, A., Kai, U. T., & Kögel-Knabner, I. (2008). Grazing effects on soil chemical and physical properties in a semiarid steppe of Inner Mongolia (P.R. China). Geoderma, 143(1–2), 63–72.
Su, Y. Z., Zhao, H. L., Zhang, T. H., & Zhao, X. Y. (2004). Soil properties following cultivation and non-grazing of a semi-arid sandy grassland in northern China. Soil and Tillage Research, 75(1), 27–36.
Su, Y. Z., Li, Y. L., Cui, J. Y., & Zhao, W. Z. (2005). Influences of continuous grazing and livestock exclusion on soil properties in a degraded sandy grassland, Inner Mongolia, northern China. Catena, 59(3), 267–278.
Wan, H. W., Bai, Y. F., Schönbach, P., Gierus, M., & Taube, F. (2010). Effects of grazing management system on plant community structure and functioning in a semiarid steppe: scaling from species to community. Plant and Soil, 340(1–2), 215–226.
Weithoff, G., Walz, N., & Gaedke, U. (2001). The intermediate disturbance hypothesis-species diversity or functional diversity? Journal of Plankton Research, 23(10), 1147–1155.
Wrage, N., Strodthoff, J., Cuchillo, H. M., Isselstein, J., & Kayser, M. (2012). Phytodiversity of temperate permanent grasslands: ecosystem services for agriculture and livestock management for diversity conservation. Biodiversity and Conservation, 20(14), 3317–3339.
Wu, G. L., Du, G. Z., Liu, Z. H., & Thirgood, S. (2009). Effect of fencing and grazing on a Kobresia-dominated meadow in the Qinghai-Tibetan Plateau. Plant and Soil, 319(2), 115–126.
Wu, G. L., Liu, Z. H., Zhang, L., Chen, J. M., & Hu, T. M. (2010). Long-term fencing improved soil properties and soil organic carbon storage in alpine swamp meadow of western China. Plant and Soil, 332(1), 331–337.
Wu, J. S., Zhang, X. Z., Shen, Z. X., Shi, P. L., Yu, C. Q., Song, M. H., & Li, X. J. (2012). Species richness and diversity of alpine grasslands on the Northern Tibetan Plateau: effects of grazing exclusion and growing season precipitation. Journal of Resources and Ecology, 3(3), 236–242.
Yang, Y. H., Fang, J. Y., Ji, C. J., & Han, W. X. (2009). Above- and belowground biomass allocation in Tibetan grasslands. Journal of Vegetation Science, 20(1), 177–184.
Yang, X., Huang, Z., Zhang, K., & Cornelissen, J. H. C. (2015). C:N:P stoichiometry of Artemisiaspecies and close relatives across northern China: unraveling effects of climate, soil and taxonomy. Journal of Ecology, 103, 1020–1031.
Zhang, Y., Dong, S. K., Gao, Q. Z., Liu, S. L., Liang, Y., & Cao, X. J. (2016). Responses of alpine vegetation and soils to the disturbance of plateau pika (Ochotona curzoniae) at burrow level on the Qinghai–Tibetan Plateau of China. Ecological Engineering, 88, 232–236.
Zhou, H. K., Tang, Y. H., Zhao, X. Q., & Zhou, L. (2006). Long-term grazing alters species composition and biomass of a shrub meadow on the Qinghai-Tibet Plateau. Pakistan Journal of Botany, 38(4), 1055–1069.
Zhu, G. Y., Deng, L., Zhang, X. B., & Shangguan, Z. P. (2016). Effects of grazing exclusion on plant community and soil physicochemical properties in a desert steppe on the Loess Plateau, China. Ecological Engineering, 90, 372–381.
Acknowledgments
This work was supported by 100 Talents Program of The Chinese Academy of Sciences, National Key R & D Program of China (2016YFC0501804), the External Cooperation Program of BIC, Chinese Academy of Sciences (No. 15175KYSB20130027), the Youth Science and Technology Innovation Team Program of Sichuan Province (2015TD0026), and the 1000 Talents Program of Sichuan Province. We thank Miss Juhua Ding, Miss Hanwei Li, Miss Peijun Ju, and Mr. Yaopu Liang for their help during field sampling. The authors give special thanks to Mr. Peng Li for his valuable comments on the figures.
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Yang, Z., Xiong, W., Xu, Y. et al. Soil properties and species composition under different grazing intensity in an alpine meadow on the eastern Tibetan Plateau, China. Environ Monit Assess 188, 678 (2016). https://doi.org/10.1007/s10661-016-5663-y
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DOI: https://doi.org/10.1007/s10661-016-5663-y