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A correlation between extractable bone morphogenetic proteins (BMPs) in demineralized bone matrix (DBM) and osteoinduction has been suggested. Extractable BMP-4 and osteoinductivity of DBM from 40 donors were assessed using enzyme-linked immunosorbent assay (ELISA) and in vivo athymic mouse assay, respectively. Extractable BMP-4 level averaged 3.7 ± 0.21 ng/g of DBM and correlated with osteoinductivity of the DBM in an in vivo assessment of induced newbone formation.
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J.H. Bennett P. Hunt P. Thorogood (1995) ArticleTitleBone morphogenetic protein-2 and 4 expression during murine orofacial development Arch. Oral Biol. 40 847–854 Occurrence Handle1:CAS:528:DyaK2MXptV2qt78%3D Occurrence Handle8651889
M.P. Bostrom J.M. Lane W.S. Berberian A.A. Missri E. Tomin A. Weiland S.B. Doty D. Glaser V. Rosen (1995) ArticleTitleImmunolocalization and expression of bone morphogenetic Proteins-2 and 4 in fracture healing J. Orthop. Res. 13 357–367 Occurrence Handle1:STN:280:DyaK2MzisF2gsw%3D%3D Occurrence Handle7602397
J.C. Cheng X. Guo L.P. Law K.M. Lee D.H. Chow R. Rosier (2002) ArticleTitleHow does recombinant human bone morphogenetic protein-4 enhance posterior spinal fusion? Spine 27 467–474 Occurrence Handle11880831
J.M. Graff (1997) ArticleTitleEmbryonic patterning: to BMP or not to BMP, that is the question Cell 89 171–174 Occurrence Handle1:CAS:528:DyaK2sXislCjsbY%3D Occurrence Handle9108472
X. Guo K.M. Lee L.P. Law H.K. Chow R. Rosier C.Y. Cheng (2002) ArticleTitleRecombinant human bone morphogenetic protein-4 (rhBMP-4) enhanced posterior spinal fusion without decortication J. Orthop. Res. 20 740–746 Occurrence Handle1:CAS:528:DC%2BD38Xltlert7c%3D Occurrence Handle12168662
C.M. Jones K.M. Lyons B.L. Hogan (1991) ArticleTitleInvolvement of Bone Morphogenetic Protein- 4 (BMP-4) and Vgr-1 in morphogenesis and neurogenesis in the mouse Development 111 531–542 Occurrence Handle1:STN:280:DyaK3Mznt1Wmsg%3D%3D Occurrence Handle1893873
L. Jortikka A. Marttinen T.S. Lindholm (1993) ArticleTitleHigh yield of osteoinductivity can be derived from demineralized bone matrix using collagenase digestion Ann. Chir. Gynaecol. 82 31–36
C.A. Kirker-Head T.N. Gerhart S.H. Schelling G.E. Henng E. Wang M.E. Holtrop (1995) ArticleTitleLong-term healing of bone using recombinant human bone morphogenetic protein-2 Clin. Orthop. 318 222–230 Occurrence Handle7671521
L.M. Leong P.M. Brickell (1996) ArticleTitleBone morphogenic protein-4 Int. J. Biochem. Cell Biol. 28 1293–1296 Occurrence Handle1:CAS:528:DyaK2sXoslSgsw%3D%3D Occurrence Handle9022288
H. Li Z. Pujic Y. Xiao P.M. Bartold (2000) ArticleTitleIdentification of bone morphogenetic proteins-2 and 4 in commercial demineralized freeze-dried bone allograft preparation: pilot study Clin. Implant. Dent. Relat. Res. 2 110–117 Occurrence Handle1:STN:280:DC%2BD3M3ntlahsA%3D%3D Occurrence Handle11359264
T. Nakase S. Nomura H. Yoshikawa J. Hashimoto S. Hirota Y. Kitamura S. Oikawa K. Ono K. Takaoka (1994) ArticleTitleTransient and localized expression of bone morphogenetic protein-4 messenger RNA during fracture healing J. Bone Miner. Res. 9 651–659 Occurrence Handle10.1002/jbmr.5650090510 Occurrence Handle1:CAS:528:DyaK2cXktVKmtrg%3D Occurrence Handle8053394
M. Niederwanger M.R. Urist (1996) ArticleTitleDemineralized bone matrix supplied by bone bank for a carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2): A substitute for autogeneic bone grafts J. Oral. Implantol. 22 210–215 Occurrence Handle1:STN:280:DyaK1c7osFWrsQ%3D%3D Occurrence Handle9524496
A. Nifuji O. Kellermann Y. Kuboki J.M. Wozney M. Noda (1997) ArticleTitlePerturbation of BMP signaling in somitogenesis resulted in vertebral and rib malformations in the axial skeletal formation J. Bone Miner. Res. 12 332–334 Occurrence Handle1:CAS:528:DyaK2sXitlKmtLg%3D Occurrence Handle9076575
A.H. Reddi (1998) ArticleTitleRole of morphogenetic proteins in skeletal tissue engineering and regeneration Nat. Biotechnol. 16 247–252 Occurrence Handle1:CAS:528:DyaK1cXhvVWls7g%3D Occurrence Handle9528003
V. Rosen K. Cox G. Hattersley (1996) Bone morphogenetic proteins J.P. Bilezikian L.G. Raisz G.A. Rodan (Eds) Principles of Bone Biology Academic Press San DiegoCAUSA 661–671
J.E. Schmidt A. Suzuki N. Ueno D. Kimelman (1995) ArticleTitleLocalized BMP-4 mediates dorsal/ventral patterning in the early Xenopus embryo Dev. Biol. 169 37–50 Occurrence Handle1:CAS:528:DyaK2MXls1Oju7g%3D Occurrence Handle7750652
Z. Schwartz A. Somers J.T. Mellonig D.L. Carnes J.M. Wozney D.D. Dean D.L. Cochran B.D. Boyan (1998) ArticleTitleAddition of human recombinant bone morphogenetic protein-2 to inactive commercial human demineralized freeze-dried bone allograft makes an effective composite bone inductive implant material J. Periodontol. 69 1337–1345 Occurrence Handle1:CAS:528:DyaK1MXhtFyjs7g%3D Occurrence Handle9926763
Y. Shigeyama J.A. D’ Errico R. Stone M.J. Somerman (1995) ArticleTitleCommercially-prepared allograft material has biological activity in vitro J. Periodontol. 66 478–487 Occurrence Handle1:STN:280:DyaK28%2Fitl2itQ%3D%3D Occurrence Handle7562337
E.M. Shore M. Xu P.B. Shah H.B. Janoff G.V. Hahn M.A. Deardorff L. Sovinsky N.B. Spinner M.A. Zasloff J.M. Wozney F.S. Kaplan (1998) ArticleTitleThe human bone morphogenetic protein-4 (BMP-4) gene: molecular structure and transcriptional regulation Calcif. Tissue Int. 63 221–229 Occurrence Handle1:CAS:528:DyaK1cXlvFWrsLc%3D Occurrence Handle9701626
Y. Watanabe N.M. Le Douarin (1996) ArticleTitleA role for BMP-4 in the development of subcutaneous cartilage Mech. Dev. 57 69–78 Occurrence Handle1:CAS:528:DyaK28Xktl2nsbk%3D Occurrence Handle8817454
Wolfinbarger L. 2001. Process for producing osteoinductive bone and osteoinductive bone produced thereby. U.S. Patent No. 6,189,537.
Wolfinbarger L. 1994. Process for demineralization of bone using column extraction. U.S. Patent No. 5,275954.
J.M. Wozney (1992) ArticleTitleThe bone morphogenetic protein family and osteogenesis Mol. Reprod. Dev. 32 160–167 Occurrence Handle1:CAS:528:DyaK38XltVCgtrk%3D Occurrence Handle1637554
J.M. Wozney V. Rosen (1998) ArticleTitleBone morphogenetic protein and bone morphogenetic protein gene family in bone formation and repair Clin. Orthop. 346 26–37 Occurrence Handle9577407
M. Xu E.M. Shore (1998) ArticleTitleMutational screening of the bone morphogenetic protein-4 gene in a family with fibrodysplasia ossificans progressiva Clin. Orthop. 346 53–58 Occurrence Handle9577410
M. Zhang R.M. Powers L. Wolfinbarger (1997a) ArticleTitleA quantitative assessment of osteoinductivity of human demineralized bone matrix J. Periodontol. 68 1076–1084 Occurrence Handle1:STN:280:DyaK1c%2FntFaqsw%3D%3D
M. Zhang R.M. Powers L. Wolfinbarger (1997) ArticleTitleEffect(s) of the demineralization process on the osteoinductivity of demineralized bone matrix J. Periodontol. 68 1085–1092 Occurrence Handle1:STN:280:DyaK1c%2FntFaqsA%3D%3D Occurrence Handle9407401
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Presented in part at the Orthopaedic Research Society 49th Annual Meeting, New Orleans, Louisiana, USA, Febuary 2–5, 2003. The authors have no conflict of interest.
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Honsawek, S., Powers, R.M. & Wolfinbarger, L. Extractable bone morphogenetic protein and correlation with induced new bone formation in an in vivo assay in the athymic mouse model. Cell Tissue Banking 6, 13–23 (2005). https://doi.org/10.1007/s10561-005-1445-4
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DOI: https://doi.org/10.1007/s10561-005-1445-4