Abstract
The antioxidant defense system in three ecotypes of reed (Phragmites communis Trin.), swamp reed (SR), dune reed (DR), and heavy salt meadow reed (HSMR), from northwest China were investigated. The HSMR possessed the highest ratio of ascorbate (ASC)/dehydroascorbate (DHA) and activities of superoxide dismutase (SOD) and catalase among the three reed ecotypes, whereas, the DR exhibited the highest ratio of glutathione/glutathione disulfide and activities of ASC peroxidase (APX) and DHA reductase. Malondialdehyde and hydrogen peroxide contents were highest in HSMR, intermediate in SR, and lowest in DR. In addition, different isoenzymes of glutathion reductase, APX, SOD and DHA were also observed in three reed ecotypes.
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Abbreviations
- APX:
-
ascorbate peroxidase (EC 1.11.1.11)
- ASC:
-
ascorbate
- CAT:
-
catalase (EC 1.11.1.6)
- DHA:
-
dehydroascorbate
- DHAR:
-
dehydroascorbate reductase (EC 1.8.5.1)
- DR:
-
dune reed
- DTT:
-
dithiothteitol
- EDTA:
-
ethylenediaminetetraacetic acid
- GR:
-
glutathione reductase (EC 1.6.4.2)
- GSH:
-
reduced glutathione
- GSSG:
-
glutathione disulfide
- HSMR:
-
heavy salt meadow reed
- MDA:
-
malondialdehyde
- MDHAR:
-
monodehydroascorbate reductase (EC 1.6.5.4)
- NBT:
-
nitroblue tetrazolium
- PAGE:
-
polyacrylamide gel electrophoresis
- POX:
-
peroxidase (EC 1.11.1.7)
- PVP:
-
polyvinylpolypyrrolidone
- SOD:
-
superoxide dismutase (EC 1.15.1.1)
- SR:
-
swamp reed
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Chen, KM., Gong, HJ., Wang, SM. et al. Antioxidant defense system in Phragmites communis Trin. ecotypes. Biol Plant 51, 754–758 (2007). https://doi.org/10.1007/s10535-007-0154-1
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DOI: https://doi.org/10.1007/s10535-007-0154-1