Abstract
The plants of pigeonpea (Cajanus cajan L.) cv. H77-216 were subjected to moderate [soil moisture content (SMC) = 7.3 ± 0.5 %] and severe (SMC = 4.3 ± 0.5 %) drought by withholding the irrigation at vegetative stage (45 d after sowing). The control plants were maintained at SMC of 11.0 ± 0.5 %. Half of the stressed plants were re-irrigated and their recovery was studied after 2 d. Leaf water potential, osmotic potential, and relative water content of leaf and root decreased significantly while a sharp rise in proline and total soluble sugars contents were noticed. Drought induced a significant increase in 1-aminocyclopropane 1-carboxylic acid (ACC) content and ACC oxidase activity which caused a considerable increase in ethylene evolution. Malondialdehyde content and relative stress injury were increased under drought whereas reverse was true for ascorbic acid content. The membrane integrity of roots decreased during stress and recovered on rehydration. The specific activity of total superoxide dismutase, ascorbate peroxidase, glutathione reductase, and glutathione transferase decreased to 37 – 78 %, 17 – 62 %, 29 – 36 % and 57 – 79 % at moderate and severe drought, respectively. The increase in activity of catalase and peroxidase could not overcome the accumulation of H2O2 content in the roots.
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Abbreviations
- AA:
-
ascorbic acid
- ACC:
-
1-aminocyclopropane-1-carboxylic acid
- ASC:
-
ascorbate
- CAT:
-
catalase
- CD:
-
critical difference
- GR:
-
glutathione reductase
- GSH:
-
glutathione
- GTase:
-
glutathione transferase
- MDA:
-
malondialdehyde
- POX:
-
peroxidase
- ROS:
-
reactive oxygen species
- RWC:
-
relative water content
- SMC:
-
soil moisture content
- SOD:
-
superoxide dismutase
- TSS:
-
total soluble sugars
- Ψs :
-
osmotic potential
- Ψw :
-
water potential
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Jain, M., Nandwal, A.S., Kundu, B.S. et al. Water relations, activities of antioxidants, ethylene evolution and membrane integrity of pigeonpea roots as affected by soil moisture. Biol Plant 50, 303–306 (2006). https://doi.org/10.1007/s10535-006-0026-0
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DOI: https://doi.org/10.1007/s10535-006-0026-0