Abstract
In contaminated soils, efficiency of natural attenuation or engineered bioremediation largely depends on biodegradation capacities of the local microflorae. In the present study, the biodegradation capacities of various microflorae towards diesel oil were determined in laboratory conditions. Microflorae were collected from 9 contaminated and 10 uncontaminated soil samples and were compared to urban wastewater activated sludge. The recalcitrance of hydrocarbons in tests was characterised using both gas chromatography (GC) and comprehensive two-dimensional gas chromatography (GC×GC). The microflorae from contaminated soils were found to exhibit higher degradation capacities than those from uncontaminated soil and activated sludge. In cultures inoculated by contaminated-soil microflorae, 80% of diesel oil on an average was consumed over 4-week incubation compared to only 64% in uncontaminated soil and 60% in activated sludge cultures. As shown by GC, n-alkanes of diesel oil were totally utilised by each microflora but differentiated degradation extents were observed for cyclic and branched hydrocarbons. The enhanced degradation capacities of impacted-soil microflorae resulted probably from an adaptation to the hydrocarbon contaminants but a similar adaptation was noted in uncontaminated soils when conifer trees might have released natural hydrocarbons. GC×GC showed that a contaminated-soil microflora removed all aromatics and all branched alkanes containing less than C15. The most recalcitrant compounds were the branched and cyclic alkanes with 15–23 atoms of carbon.
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References
Beam HW, Perry JJ, (1974) Microbial degradation of cycloparaffinic hydrocarbons via co-metabolism and commensalismJ. Gen. Microbiol. 82: 163–169
Beens J, Adahchour M, Vreuls RJJ, van Alterna K, Brinkman UA, (2001) Simple, non-moving modulation intrerface for comprehensive two-dimensional gas chromatography J. Chromatogr. A 919: 127–132
Bertsch W, (1999) Two-dimensional gas chromatography. Concepts, instrumentation, and applications – Part 1: fundamentals, conventional two-dimensional gas chromatography, selected applicationsJ. High Resol. Chromatogr. 22: 647–665
Bertsch W, (2000) Two-dimensional gas chromatography. Concepts, instrumentation, and applications – Part 2: fundamentals, comprehensive two-dimensional gas chromatographyJ. High Resol. Chromatogr. 23: 167–181
Blomberg J, Schoenmakers PJ, Brinkman UA, (2002) Gas chromatographic methods for oil analysisJ. Chromatogr. A 972:137–173
Bouchez M, Blanchet D, Vandecasteele JP, (1995) Degradation of polycyclic aromatic hydrocarbons by pure strains and by defined strain associations: inhibition phenomena and cometabolismAppl. Microbiol. Biotechnol. 43:156–164
Cerniglia CE, Perry JJ, (1973) Crude oil degradation by microorganisms isolated from the marine environmentZ. Allg. Mikrobiol. 13:299–306
Chaillan F, Le Fleche A, Bury E, Phantavong YH, Grimont P, Saliot A, Oudot J, (2004) Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganismsRes. Microbiol. 155: 587–595
Delille D, Coulon F, Pelletier E, (2004) Biostimulation of natural microbial assemblages in oil-amended vegetated and desert sub-Antarctic soilsMicrob. Ecol. 47: 407–415
Di Sanzo FP, Lane JL, Rey RE, (1988) Application of the state-of-the-art multidimensional high resolution gas chromatography for individual component analysisJ. Chromatogr. Sci. 26: 206–209
Ducreux J, Lafargue E, Bocard C, Marquis F, Pillot D, (1997) Utilisation de la méthode Rock-Eval pour l’évaluation des sols contaminés par des composés hydrocarbonés Analusis 25: 46–50
Frysinger GF, Gaines RB, Xu L, Reddy CM, (2003) Resolving the unresolved complex mixture in petroleum-contaminated sedimentsEnviron. Sci. Technol. 37: 1653–1662
Gallego JLR, Loredo J, Llamas JF, Vazquez F, Sanchez J, (2001) Bioremediation of diesel-contaminated soils: evaluattion of potential in situ techniques by study of bacterial degradationBiodegradation 12: 325–335
Horowitz A, Atlas RM, (1977) Response of microorganisms to an accidental gasoline spillage in an artic freshwater ecosystemAppl. Environ. Microbiol. 33:1252–1258
Leahy JG, Colwell RR, (1990) Microbial degradation of hydrocarbons in the environmentMicrobiol. Rev. 54: 305–315
McKenna EJ, Kallio RE, (1971) Microbial metabolism of the isoprenoid alkane pristaneProc. Natl. Acad. Sci. USA 68: 1552–1554
Mondello L, Lewis AC & Bartle KD (2001) Multidimensional chromatography. John Wiley & Sons, Ltd
Morgan P, Watkinson RJ, (1989) Hydrocarbon degradation in soils and methods for soil biotreatmentCrit. Rev. Biotechnol. 8: 305–333
Musy A & Soutter M (1991) Physique du sol. Collection Gérer l’Environnement, Presse polytechniques et universitaires romandes
NF ISO 10390 (2005) Soil quality – Determination of pH
NF ISO 11465 (1993) Soil quality – Determination of dry matter and water content basis – Gravimetric method
Olson JJ, Mills G, Herbert BE, Morris PJ, (1999) Biodegradation rates of separated diesel componentsEnviron. Toxicol. Chem. 18: 2448–2453
Penet S, Marchal R, Sghir A, Monot F, (2004) Biodegradation of hydrocarbon cuts used for diesel oil formulation Appl. Microbiol. Biotechnol. 66: 40–47
Pirnik MP, Atlas RM, Bartha R, (1974) Hydrocarbon metabolism by Brevibacterium erythrogenes: normal and branched alkanes J. Bacteriol. 119: 868–878
Röling WF, van Verseveld HW, (2002) Natural attenuation: what does the subsurface have in store? Biodegradation 13: 53–64
Salanitro JP, (2001) Bioremediation of petroleum hydrocarbons in soil Adv. Agron. 72:53–105
Solano-Serena F, Marchal R, Lebeault JM, Vandecasteele JP, (2000) Distribution in the environment of degradative capacities for gasoline attenuationBiodegradation 11: 29–35
Solano-Serena F, Marchal R, Ropars M, Lebeault JM, Vandecasteele JP, (1999) Biodegradation of gasoline: kinetics, mass balance and fate of individual hydrocarbonsJ. Appl. Microbiol. 86: 1008–1016
Teng S.T., Williams A.D, Urdal K, (1994) Detailed hydrocarbon analysis of gasoline by GC-MS (Sl-PIONA) J. High. Resol. Chromatogr. 17: 469–475
Van Deursen M, Beens J, Reijenga J, Lipman P, Cramers C, Blomberg J, (2000) Group-type identification of oil samples using comprehensive two-dimensional gas chromatography coupled to a time-of-flight mass spectrometer J. High. Resol. Chromatogr. 23:507–510
Van Hamme JD, Singh A, Ward OP, (2003) Recent advances in petroleum microbiology Microbiol. Mol. Biol. Rev. 67: 503–549
Vendeuvre C, Ruiz-Guerrero R, Bertoncini F, Duval L, Thiébaut D & Hennion MC (2005) Characterisation of middle-distillates by comprehensive two-dimensional gas chromatography (GC×GC): A powerful alternative for performing various standard analysis of middle-distillates. J. Chromatogr. A 1086: 21–28
Ward O, Singh A, Van Hamme J, (2003) Accelerated biodegradation of petroleum hydrocarbon waste J. Ind. Microbiol. Biotechnol. 30: 260–270
Acknowledgements
The skilful assistance of Véronique Bardin in GC analyses and that of Laurent Duval in developing GC×GC data processing are acknowledged.
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Penet, S., Vendeuvre, C., Bertoncini, F. et al. Characterisation of biodegradation capacities of environmental microflorae for diesel oil by comprehensive two-dimensional gas chromatography. Biodegradation 17, 577–585 (2006). https://doi.org/10.1007/s10532-005-9028-4
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DOI: https://doi.org/10.1007/s10532-005-9028-4