Abstract
High-fat diet (HFD)-induced obesity is associated with oxidative stress. The purpose of this study was to examine the antioxidant effect of Phaeodactylum tricornutum extract in mice with diet-induced obesity. Four-week-old C57BL/6J mice were fed a normal diet or HFD with and without 0.7% P. tricornutum lipid extract corresponding to 0.2% fucoxanthin for 8 weeks. P. tricornutum significantly decreased body weight and epidydimal white adipose tissue in mice fed the HFD. Serum triglyceride, glucose, insulin, and leptin levels, as well as homeostasis model assessment for insulin resistance (HOMA-IR) values, were significantly lower in the P. tricornutum group than in the HFD group. P. tricornutum significantly decreased thiobarbituric acid reactive substances (TBARS) and increased glutathione and the activities of superoxide dismutase, catalase, and glutathione peroxidase in the liver compared with the HFD group. Thus, P. tricornutum could exert antiobesity and antioxidant effects in mice fed a HFD.
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Calle EE, Thun MJ, Petrelli JM, Rodriguez C, Heath CW. Bodymass index and mortality in a prospective cohort of US adults. New Engl. J. Med. 341: 1097–1105 (1999)
Kim KY, Lee HN, Kim YJ, Park T. Garcinia cambogia extract ameliorates visceral adiposity in C57BL/6J mice fed on a high-fat diet. Biosci. Biotech. Bioch. 72: 1772–1780 (2008)
Maeda H, Hosokawa M, Sashima T, Murakami-Funayama K, Miyashita K. Anti-obesity and anti-diabetic effects of fucoxanthin on diet-induced obesity conditions in a murine model. Mol. Med. Rep. 2: 897–902 (2009)
Woo MN, Jeon SM, Kim HJ, Lee MK, Shin SK, Shin YC, Park YB, Choi MS. Fucoxanthin supplementation improves plasma and hepatic lipid metabolism and blood glucose concentration in high-fat fed C57BL/6N mice. Chem.-Biol. Interact. 186: 316–322 (2010)
Milagro FI, Campión J, Martínez JA. Weight gain induced by high-fat feeding involves increased liver oxidative stress. Obesity 14: 1118–1123 (2006)
Kang MY, Kim SM, Rico CW, Lee SC. Hypolipidemic and antioxidative effects of rice bran and phytic acid in high fat-fed mice. Food Sci. Biotechnol. 21: 123–128 (2012)
Higdon JV, Frei B. Obesity and oxidative stress: A direct link to CVD? Arterioscl. Throm. Vas. 23: 365–367 (2003)
Haugan JA, Liaaen-Jensen S. Naturally occurring stereoisomers of fucoxanthin. Phytochemistry 31: 1359–1361 (1992)
Maeda H, Hosokawa M, Sashima T, Funayama K, Miyashita K. Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochem. Bioph. Res. Co. 332: 392–397 (2005)
Jeon SM, Kim HJ, Woo MN, Lee MK, Shin YC, Park YB, Choi MS. Fucoxanthin-rich seaweed extract suppresses body weight gain and improves lipid metabolism in high-fat-fed C57BL/6J mice. Biotechnol. J. 5: 961–969 (2010)
Hosokawa M, Miyashita T, Nishikawa S, Emi S, Tsukui T, Beppu F, Okada T, Miyashita K. Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-Ay mice. Arch. Biochem. Biophys. 504: 17–25 (2010)
Kim SM, Jung YJ, Kwon ON, Cha KH, Um BH, Chung D, Pan CH. A potential commercial source of fucoxanthin extracted from the microalga Phaeodactylum tricornutum. Appl. Biochem. Biotech. 166: 1843–1855 (2012)
Bertrand M. Carotenoid biosynthesis in diatoms. Photosynth. Res. 106: 89–102 (2010)
Sachindra NM, Sato E, Maeda H, Hosokawa M, Niwano Y, Kohno M, Miyashita K. Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites. J. Agr. Food Chem. 55: 8516–8522 (2007)
Kikuchi M, Hirano A, Kunito S, Kawakami Y. Fucoxanthin, an antioxidative substance from marine diatom Phaeodactylum tricornutum. J. Mar. Biotechnol. 3: 132–135 (1995)
Yan X, Chuda Y, Suzuki M, Nagata T. Fucoxanthin as the major antioxidant in Hijikia fusiformis, a common edible seaweed. Biosci. Biotech. Bioch. 63: 605–607 (1999)
Heo SJ, Ko SC, Kang SM, Kang HS, Kang HS, Kim JP, Kim SH, Lee KW, Cho MG, Jeon YJ. Cytoprotective effect of fucoxanthin isolated from brown algae Sargassum siliquastrum against H2O2-induced cell damage. Eur. Food Res. Technol. 228: 145–151 (2008)
Liu CL, Huang YS, Hosokawa M, Miyashita K, Hu ML. Inhibition of proliferation of a hepatoma cell line by fucoxanthin in relation to cell cycle arrest and enhanced gap junctional intercellular communication. Chem.-Biol. Interact. 182: 165–172 (2009)
Haffner SM, Miettinen H, Stern MP. The homeostasis model in the San Antonio heart study. Diabetes Care 20: 1087–1092 (1997)
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351–358 (1979)
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254 (1976)
Ellman GL. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82: 70–77 (1959)
Aebi H. Catalase. Vol. 2, pp. 673–684. In: Methods in Enzymatic Analysis. Academic Press, New York, NY, USA (1974)
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70: 158–169 (1967)
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47: 469–474 (1974)
Maeda H, Hosokawa M, Sashima T, Miyashita K. Dietary combination of fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese/diabetic KK-Ay mice. J. Agr. Food Chem. 55: 7701–7706 (2007)
Park HJ, Lee MK, Park YB, Shin YC, Choi MS. Beneficial effects of Undaria pinnatifida ethanol extract on diet-induced-insulin resistance in C57BL/6J mice. Food Chem. Toxicol. 49: 727–733 (2011)
Woo MN, Jeon SM, Shin YC, Lee MK, Kang MA, Choi MS. Antiobese property of fucoxanthin is partly mediated by altering lipidregulating enzymes and uncoupling proteins of visceral adipose tissue in mice. Mol. Nutr. Food Res. 53: 1603–1611 (2009)
Matsumoto M, Hosokawa M, Matsukawa N, Hagio M, Shinoki A, Nishimukai M, Miyashita K, Yajima T, Hara H. Suppressive effects of the marine carotenoids, fucoxanthin, and fucoxanthinol on triglyceride absorption in lymph duct-cannulated rats. Eur. J. Nutr. 49: 243–249 (2010)
Pelleymounter MA, Cullen MJ, Baker MB, Hecht R, Winters D, Boone T, Collins F. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269: 540–543 (1995)
Matsuzawa Y. The metabolic syndrome and adipocytokines. FEBS Lett. 22: 2917–2921 (2006)
Kahn SE, Prigeon RL, Schwartz RS, Fujimoto WY, Knopp RH, Brunzell JD, Porte D Jr. Obesity, body fat distribution, insulin sensitivity, and islet β-cell function as explanations for metabolic diversity. J. Nutr. 131: 354S–360S (2001)
Nishikawa S, Hosokawa M, Miyashita K. Fucoxanthin promotes translocation and induction of glucose transporter 4 in skeletal muscles of diabetic/obese KK-A(y) mice. Phytomedicine 19: 389–394 (2012)
Matsuzawa-Nagata N, Takamura T, Ando H, Nakamura S, Kurita S, Misu H, Ota T, Yokoyama M, Honda M, Miyamoto K, Kaneko S. Increased oxidative stress precedes the onset of high-fat dietinduced insulin resistance and obesity. Metabolism 57: 1071–1077 (2008)
Townsend DM, Tew KD, Tapiero H. The importance of glutathione in human disease. Biomed. Pharmacother. 57: 145–155 (2003)
Droge W. Free radicals in the physiological control of cell function. Physiol. Rev. 82: 47–95 (2002)
Marxen K, Vanselow KH, Lippemeier S, Hintze R, Ruser A, Hansen UP. Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements. Sensors 7: 2080–2095 (2007)
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Kang, MJ., Kim, S.M., Jeong, SM. et al. Antioxidant effect of Phaeodactylum tricornutum in mice fed high-fat diet. Food Sci Biotechnol 22, 107–113 (2013). https://doi.org/10.1007/s10068-013-0015-y
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DOI: https://doi.org/10.1007/s10068-013-0015-y