Abstract
Seven yeast recombinants were selected from 57 fusant colonies on the basis of higher DNA content, nuclear diameter and ethanol yield compared to parental Saccharomyces cerevisiae ATCC 4126 and Kluyveromyces lactis CBS 683. Six recombinants out of the above colonies revealed growth on lactose and sucrose, indicating that they are S. cerevisiae with transformed β-galactosidase systems. The fusant colonies were investigated with respect to their capacity to convert lactose in salted cheese whey into ethanol. Among these recombinants that showed high tolerance towards sodium chloride and higher ethanol yield than lactose fermenting parental K. lactis CBS 683, SK-1 exhibited high tolerance up to 4 g dl–1 sodium chlonride with an ethanol yield of 4.66 ml dl–1 (v/v) , SK-23 tolerated 6 g dl–1 sodium chloride with an ethanol yield of 4.14 ml dl–1 (v/v) and SK-26 showed resistance towards 8 g dl–1 sodium chloride and give an ethanol yield of 3 ml dl–1 (v/v).
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Received: 23 January 1998
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Tahoun, M., El-Nemr, T. & Shata, O. Ethanol from lactose in salted cheese whey by recombinant Saccharomyces cerevisiae. Z Lebensm Unters Forsch 208, 60–64 (1999). https://doi.org/10.1007/s002170050376
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DOI: https://doi.org/10.1007/s002170050376