Abstract
Abstract
Objective. Excess nitric oxide (NO) and its reactive derivatives cause oxidative reactions that lead to cell death. Propofol, an intravenous anesthetic, exhibits antioxidant properties. Diprivan is a widely used commercial preparation of propofol that is emulsified in 10% intralipids. We sought to test the hypothesis that clinically encountered concentrations of Diprivan attenuate the toxicity of NO in a cell culture model.
Design. Prospective, randomized, controlled trial.
Setting. University research laboratory.
Subjects. Cultured human bronchial epithelial (IB-3) cells.
Interventions. Human bronchial epithelial cell cultures were randomly assigned to one of the following six groups: no additives (negative control), NO alone (positive control), NO with either 1 µM, 10 µM or 100 µM Diprivan, and 100 µM Diprivan alone (Diprivan control). S-nitroso-N-acetylpenicillamine (SNAP) was used to generate NO.
Measurements and results. Hemacytometry with trypan blue staining was used to measure cell survival. To assess direct NO toxicity, immunoblot assays for nitrotyrosine-containing proteins in cell homogenates were performed. Exogenous NO significantly decreased live cell numbers and increased intracellular nitrotyrosine-containing protein concentrations (p<0.001). Diprivan significantly attenuated these changes in a concentration-independent manner (p<0.001). At concentrations as low as 1 µM, Diprivan exhibited cytoprotective effects.
Conclusions. Diprivan effectively attenuates the cytotoxicity of excessive NO exposure in IB-3 cells at concentrations that are clinically attainable.
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Huang, C., Slovin, P.N., Nielsen, R.B. et al. Diprivan attenuates the cytotoxicity of nitric oxide in cultured human bronchial epithelial cells. Intensive Care Med 28, 1145–1150 (2002). https://doi.org/10.1007/s00134-002-1380-9
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DOI: https://doi.org/10.1007/s00134-002-1380-9