Abstract
Successful establishment of sepsis by entrapping a dose of 150 colony forming units ofKlebsiella pneumoniae in a fibrin clot following implantation into the peritoneal cavity of mice is reported. The dose in the fibrin clot gave 50 % mortality in mice, spread over a period of one week. All the infected mice showed positive blood culture up to 6 d post-infection; histopathology revealed inflammatory changes in both liver and spleen. Introduction ofK. pneumoniae into experimental mice without entrapment in fibrin clot caused no mortality and blood culture remained positive only up to 2 d; histopathology of liver and spleen throughout the period of study showed relatively mild inflammatory changes, which almost cleared during 14 d post-infection. The use of the fibrin-clot model may thus be considered to be useful in studying both the initial and the persisting stage of infection in the peritoneum, whence a slow release of bacteria into the blood takes place which finally leads to sepsis and septicemia.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Alexander J.W., Gonce S.J., Miskell P.W., Peck M.D., Saw H.: A new model for studying nutrition in peritonitis: the adverse effect of overfeeding.Ann.Surg. 209, 334–338 (1989).
Asheg A.A., Fedorova V., Pistl J., Levkut M., Revajová V., Kolodzieyski L., Ševčiková Z., Pilipčinec E.: Effect of low and high doses ofSalmonella enteritidis PT4 on experimentally infected chicks.Folia Microbiol. 46, 459–462 (2001).
Bucklin S.E., Morrison D.C.: Differences in therapeutic efficacy among cell wall-active antibiotics in a mouse model of Gram-negative sepsis.J.Infect.Dis. 172, 1519–1527 (1995).
DeMarsh P.L., Wells G.I., Thomas F., Lewamdowski T.F., Frey C.L., Bhatnagar P.K., Ostovie E.J.R.: Treatment of experimental Gram-negative and Gram-positive bacterial sepsis with the hematoregulatory peptide SK&F 107647.J.Infect.Dis. 173, 203–211 (1996).
Fink M.P., MacVittie T.J., Casey L.C.: Inhibition of prostaglandin synthesis restores normal hemodynamics in canine hyperdynamic sepsis.Ann.Surg. 200, 619–626 (1984).
Kondo S., Shinagawa A., Saito T., Kiyosawa H., Yamanaka I., Aizawa K., Fukuda S., Hara A., Itoh A., Kawai J., Shibata K., Hayashizaki Y.: Computational analysis of full length mouse cDNAs compared with human genome sequences.Mamm.Genome 12, 673–677 (2001).
Mayeus P.R.: Pathobiology of lipopolysaccharide.J.Toxicol. 51, 415–435 (1997).
Melissen P.M.B., Vianen W.V., Bakker-Wouden Berg I.A.J.M.: Treatment ofKlebsiella pneumoniae septicemia in normal and leukopenic mice by liposome-encapsulated muramyl tripeptide phosphatidylethanolamide.Antimicrob.Agents Chemother. 38, 147–150 (1994).
Mural R.J., Adams M.D., Myers E.W., Smith H.O., Miklos G.L., Wides R., Halpern A., Li P.W., Sutton G.G., Nadeau J., Salzberg S.L., Holt R.A., Kodira C.D., Lu F., Chen L., Deng Z., Evangelista C.G., Gan W., Heiman T.J., Li J., Li Z., Merkulov G.V., Milshina N.V., Naik A.K., Qi R., Shue B.C., Wang A., Wang J., Wang X., Yan X., Ye J., Yooseph S., Zhao Q., Zheng L., Zhu S.C., Biddick K., Bolanos R., Delcher A.L., Dew I.M., Fasulo D., Flanigan M.J., Huson D.H., Kravitz S.A., Miller J.R., Mobarry C.M., Reinert K., Remington K.A., Zhang Q., Zheng X.H., Nussken D.R., Lai Z., Lei Y., Zhong W.: A comparison of whole-genome shotgun-derived mouse chromosome 16 and human genome.Science 296, 166–6171 (2002).
Nabber P., Mikelsaar R.H., Salminen S., Mikelsaar M.: Bacterial translocation, intestinal microflora and morphological changes of intestinal mucosa in experimental models ofClostridium difficile infection.J.Med.Microbiol. 47, 591–598 (1998).
Naaber P., Smidt I., Tamme K., Lhgant A., Tapfer H., Mikelsaar M., Talvik R.: Translocation of indigenous microflora in an experimental model of sepsis.J.Med.Microbiol. 49, 431–439 (2000).
Natanson C., Danner R.L., Elin R.J.: Role of endotoxemia in cardiovascular dysfunction and mortality,Escherichia coli andStaphylococcus aureus challenges in a canine model of human septic shock.J.Clin.Invest. 83, 243–251 (1989).
Orskov I.: GenusKlebsiella, pp. 461–465 in N.R. Kreig, J.G. Holt (Eds):Bergey’s Manual of Systematic Bacteriology, Vol. 1. Williams and Wilkins, Baltimore (USA) 1984.
Waldon E., Sobis-Glinkowska M., Szewczyk E.M.: Evaluation of selected features ofStaphylococcus cohnii enabling colonization of humans.Folia Microbiol. 47, 565–572 (2002).
Wells C.L.: Relationship between intestinal microecology and the translocation of intestinal bacteria.Antonie van Leeuwenhoek 58, 87–93 (1990).
Wichterman K.A., Baue A.E., Chaudry I.H.: Sepsis and septic shock — a review of laboratory models and a proposal.J.Surg.Res. 29, 189–201 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Toky, V., Sharma, S., Arora, B.B. et al. Establishment of a sepsis model following implantation ofKlebsiella pneumoniae-infected fibrin clot into the peritoneal cavity of mice. Folia Microbiol 48, 665–669 (2003). https://doi.org/10.1007/BF02993476
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02993476