Abstract
Mouse models of bacterial sepsis are widely used in research to investigate the underlying molecular mechanisms of sepsis and to develop clinically useful therapeutic regimens. Three commonly used mouse sepsis models include (a) injection of bacterial endotoxin, (b) infusion of cultured bacteria, and (c) cecal ligation and puncture. Here we describe the induction of bacterial sepsis in mice by intraperitoneal injection of cultured live Escherichia coli cells. The severity of the sepsis can be regulated by the number of E. coli cells injected into the peritoneal cavity of mice.
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Acknowledgments
This work was supported by the grants from the National Natural Science Foundation of China (Nos. 81773965, 81673664, and 81373423).
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He, XH., Ouyang, DY., Xu, LH. (2021). Injection of Escherichia coli to Induce Sepsis. In: Walker, W.E. (eds) Sepsis. Methods in Molecular Biology, vol 2321. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1488-4_5
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DOI: https://doi.org/10.1007/978-1-0716-1488-4_5
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1488-4
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