Abstract
A HPLC method with UV/diode array detection for the determination of deoxynivalenol (DON) and deepoxy-deoxynivalenol (DOM-1) in milk was developed. Milk was incubated with β-glucuronidase and then defatted. After purification by immunoaffinity chromatography, DON and DOM-1 were separated on a C18 reversed phase column with acetonitril/water (10/90) as the mobile phase and detected at 218 nm. Limits of quantification were 1 μg/l for both toxins, with mean recoveries (1–10 μg/l) of 97% (DON) and 84% (DOM-1), respectively. Milk samples (pasteurized, UHT; n=32) from German retail shops were analysed by this method. Neither DON/DOM-1 nor their glucuronides were found in any sample. These results are consistent with published studies indicating that in lactating cows, DON and DOM-1 are mostly eliminated through urine, and that the carry-over into milk is negligible.
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Financial support Bundesministerium fur Verbrauchersuputz, Emanrung und Landwirtschaft (BIF 00 HS 055)
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Curtui, V., Seidler, C., Schneider, E. et al. Bestimmung von Deoxynivalenol und Deepoxy-Deoxynivalenol in Milch. Mycotox Res 21, 40–42 (2005). https://doi.org/10.1007/BF02954814
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DOI: https://doi.org/10.1007/BF02954814