Abstract
The prevalence of extended-spectrum β-lactamases (ESBL) was determined among isolates ofEscherichia coli (n=63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21 %) were ESBL-positive for two screening tests, the double disk-synergy test and theOxoid Combination Disk method. One ESBL-positive isolate came from a healthy child. The transfer frequency of oxyimino-β-lactam resistance from ESBL-producing isolates toE. coli K12 C600 recipient strain ranged from 10−8 to 10−5 per donor cell. Donor strains and transconjugants displayed susceptibility patterns typical of ESBL producers. They were resistant to oxyimino-β-lactams but susceptible to clavulanic acid and carbapenems. Seven out of the 10 ESBL-positive isolates were found to produce MR/MS fimbria, which may play an important role in the colonization of the human intestinal mucosa.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- CFU:
-
colony forming unit
- ESBL:
-
extended-spectrum β-lactamases
- DDST:
-
double disk-synergy test
- MR:
-
mannose-resistant (fimbria)
- MS:
-
mannose-sensitive (fimbria)
- OBLR:
-
oxyimino-β-lactam resistance
- OCDM:
-
Oxoid Combination Disk method
- Amc:
-
amoxicillin
- Ctx:
-
ceftriaxone
- Cla:
-
clavulanate
- Azt:
-
aztreonam
- Imi:
-
imipenem
- Cef:
-
cefotaxime
- Mer:
-
meropenem
- Cfz:
-
ceftazidime
- Pip:
-
piperacillin
- Cpd:
-
cefpodoxime
- Nal:
-
nalidixic acid
References
Bogyiová E., Siegfried L., Kmeťová M., Šandorčinová Z., Liptakova A., Biroš E.: Occurrence and genetic association of selected virulence factors in clinicalEscherichia coli isolates.Folia Microbiol.47, 73–77 (2002).
Bujdaková H., Hanzen J., Jankovičová S., Klimáčková J., Moravčíková M., Milošovič P., Michalková-Papajová D., Kallová J., Jakab A., Kettner M.: Occurrence and transferability of β-lactam resistance inEnterobacteriaceae isolated inChildren’s University Hospital in Bratislava.Folia Microbiol.46, 339–344 (2001).
Carter M.W., Oakton K.J., Warner M., Livermore D.M.: Detection of extended-spectrum β-lactamases in klebsiellae with the Oxoid combination disk method.J.Clin.Microbiol.38, 4228–4232 (2000).
Duguid J.P., Clegg S., Wilson M.J.: The fimbrial and non-fimbrial hemagglutinins ofEscherichia coli.J.Infect.Microbiol.12, 213–227 (1987).
Hacker J.: Role of fimbrial adhesins in the pathogenesis ofEscherichia coli infections.Can.J.Microbiol.38, 720–727 (1992).
Jacoby G.A., Medeiros A.A.: More extended-spectrum β-lactamases.Antimicrob.Agents Chemother.35, 1697–1704 (1991).
Jarlier V., Nicolas M.H., Fournier G., Philippon A.: Extended broad-spectrum β-lactamases conferring transferable resistance to newer β-lactam agents inEnterobacteriaceae: hospital prevalence and susceptibility patterns.Rev.Infect.Dis.10, 867–878 (1988).
Knothe H., Shah P., Krcmery V., Mitsuhashi A.S.: Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates ofKlebsiella pneumonae andSerratia marcescens.Infection11, 315–317 (1983).
Levine M.M.:Escherichia coli that cause diarrhea.J.Infect.Dis.155, 377–389 (1987).
Liptáková A., Siegfried L., Podracká L’., Sabol M., Sehnálková H., Bogyiová E., Rosocha J., Kmeťová M., Kerestešová H., Kotulová D.: Detection of shiga toxins, intimin and enterohemolysin inEscherichia coli strains isolated from children in eastern Slovakia.Folia Microbiol.47, 185–188 (2002).
Livermore D.M.: β-Lactamases in laboratory and clinical resistance.Clin.Microbiol.Rev.8, 557–584 (1995).
Livermore D.M., Yuan M.: Antibiotic resistance and production of extended-spectrum β-lactamases amongstKlebsiella spp. from intensive care units in Europe.J.Antimicrob.Chemother.38, 409–424 (1996).
Majtán V., Majtánová L’.: Influence onEnterobacter cloacae metabolism, cell-surface hydrophobicity and motility of suprainhibitory concentrations of carbapenems.Folia Microbiol.46, 505–510 (2001).
Medeiros A.A.: Evolution and dissemination of β-lactamases accelerated by generations of β-lactam antibiotics.Clin.Infect.Dis.24 (Suppl. 1), 19–45 (1997).
Nataro J.P., Kaper J.B.: DiarrheagenicEscherichia coli.Clin.Microbiol.Rev.11, 142–201 (1998).
National Committee for Clinical Laboratory Standards, Performance Standards for Antimicrobial Susceptibility Testing, 11th Informational Supplement M100-S11. NCCLS, Wayne (USA) 2001.
Pagani L., Migliavacca R., Luzzaro F., Giacobone E., Perilli M., Micheletti P., Amicosante G.: Comparative activity of piperacillin/tazobactam against clinical isolates of extended-spectrum β-lactamase-producingEnterobacteriaceae.Chemotherapy44, 377–384 (1998).
Stobberingh E.E., Arends J., Hoogkamp-Korstanje J.A.A., Goessens W.H.F., Visser M.R., Buiting A.G.M., Debets-Ossenkopp Y.J., van Ketel R.J., van Ogtrop M.L., Stabbe L.J.M., Voorn G.P., Winter H.L.J., van Zeijl J.H.: Occurrence of extended-spectrum β-lactamases (ESBL) in Dutch hospitals.Infection6, 348–354 (1999).
Tejedor-Junco M.T., Gonzalez-Martin M., Lupiola P., Gonzalez-Lama Z.: Type-I β-lactamases ofEnterobacter cloacae and resistance to β-lactam antibiotics.Folia Microbiol.43, 683–686 (1998).
Vahaboglu H., Coskunkan F.M., Tansel O., Ozturk R., Sahin N., Koksal I., Kocazeybek B., Tatman-Otkun M., Leblebicioglu H., Ozinel M.A., Akalin H., Kocagoz S., Korten V.: Clinical importance of extended-spectrum-β-lactamase (PER-I-type)-producingAcinetobacter baumannii andPseudomonas aeruginosa strains.J.Med.Microbiol.50, 642–645 (2001).
Waldon E., Sobis-Glinkowska M., Szewczyk E.M.: Evaluation of selected features ofStaphylococcus cohnii enabling colonization of humans.Folia Microbiol.47, 565–572 (2002).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Franiczek, R., Sobieszczańska, B., Grabowski, M. et al. Occurrence of extended-spectrum β-lactamases amongEscherichia coli isolates from hospitalized and healthy children. Folia Microbiol 48, 243–247 (2003). https://doi.org/10.1007/BF02930963
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02930963