Abstract
Greenhouse assays were carried out to characterizeSolanum accessions previously reported to be late blight resistant and to identify individuals within the accessions with high levels of resistance to late blight. Foliage of wildSolanum species accessions or hybrid cultivated potato x wild species (diploid or tetraploid) from Mexico, Russia, or South America was inoculated withPhytophthora infestans (US-8) and was scored for severity of foliar symptoms at 7 and 14 days after inoculation (DAI). Mexican accessions (S. brachycarpum,S. pinnatisectum,S. guerreroense, andS.fendleri) were significantly more resistant (1.1% infection at 14 DAI) than either Russian (59.1% infection at 14 DAI) or South American (53.4% infection at 14 DAI) accessions. Moreover, the genotypes within the Mexican accessions tended to be more uniformly resistant whereas genotypes within the Russian and South American accessions tended to segregate for resistance. The more resistant genotypes of Russian and South American accessions (176/546) were retested, and 56 genotypes were identified as having potential for use in potato breeding programs for resistance to late blight. Fifty percent of the selected genotypes were fromS. microdontum accessions PI498124, PI595510, and PI595511. The potential of these species for incorporation into breeding programs for late blight resistance is discussed.
Resumen
Un experimente fue conducido en invernadero para la seleción de genoti.pos entre accesiones deSolanum previamente identificados como resistentes al tizón tardÌo de la papa (Phytophthora infestans). El foliaje de accesiones de especies silvestres o hibridos entre cultivares de papa y especies silvestres (diploide y tetraploide) de MÈxico, Rusia y America del Sur fue inoculado con el genotipo US-8 de P.infestans. El porcentaje del foliage infectado fue evaluado a los 7 y 14 dias despues de la inoculación. Las accesiones de MÈxico (S. brachycarpum,S. pinnatisectum,S. guerreroense yS. fendleri) fueron significativamente mas resistentes al tizón tardÌo a los 14 dias depues de la inoculación (1,1%) que las accesiones de Rusia (59,1% ) o de America der Sur (53,4% ). Tambien, la resistencia al tizón tardÌo fue uniformemente distribuida al nivel de genotipo en las accesiones de MÈxico, pero segregación para resistancia ocurrio en accesiones de Rusia y de America del Sur. Selecciones hechas en las accesiones de Rusia y de America del Sur (176/546) fueron reevaluadas en 1998 y resultarón en 56 genotipos que pueden ser utilizados en un programa de mejoramiento para resistencia ai tizón tardÌo de la papa. Un total de 50% de los genotipos seleccionados sonSolanum microdontum accesiones PI 498124, PI 595510 y PI 595511. Tambien se discuten las posibilidades del uso y las estrategias de mejoramiento para la utilizatión de estas especies en un programa de resistencia al tizón tardÌo de la papa.
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Literature Cited
Bamberg, J.B., M.W. Martin, and J.J. Schartner. 1994a Elite selections of tuber-bearingSolanum species germplasm.In: Inter-Regional Potato Introduction Station, NRSP-6, Sturgeon Bay, WI. pp. 14–15
Bamberg, J.B., R.E. Hanneman, J.P. Palta, and J.F. Harbage. 1994b. Using disomic 4x(2EBN) potato species’ germplasm via bridge speciesSolanum commersonii. Genome 37:866–870.
Bamberg, J.B., C.A. Longtine, and E.B. Radcliffe. 1996. Fine screeningSolanum (potato) germplasm accessions for resistance to Colorado potato beetle. Am Potato J 73:211–223.
Black, W. 1970. The nature and inheritance of field resistance to late blight (Phytophthora infestans) in potatoes. Am Potato J 47:279–288.
Colon, L.T. and D.J. Budding. 1988. Resistance to late blight(Phytophthora infestans) in ten wildSolanum species. Euphytica [Suppl]: 77–86.
Colon, L.T., D.J. Budding, L.C.P. Keizer, and J. Pieters. 1995. Components of resistance to late blight(Phytophthora infestans) in eight South AmericanSolanum species. Euro J Plant Path 101:441–456.
Deahl, K.L., D.A. Inglis, and S.P. Demuth. 1993. Testing for resistance to metalaxyl inPhytophthora infestans isolates from northwestern Washington. Am Potato J 70:779–795.
Douches, D.S., W.W. Kirk, K. Jastrzebski, C. Long, and R. Hammerschmidt. 1997. Susceptibility of potato varieties and advanced breeding lines(Solanum tuberosum L.) toPhytophthora infestans (Mont.) de Bary in greenhouse screenings. Am Potato J 74:75–86.
Goodwin, S.B., R.E. Schneider, and W.E. Fry. 1995. Use of cellulose-acetate electrophoresis for rapid identification of allozyme genotypes ofPhytophthora infestans. Plant Dis 79:1181–1185.
Hamm, P.B., B.A. Fry, and J. Jaeger. 1994. Occurrence and frequency of metalaxyl insensitivity and mating types ofPhytophthora infestans in the Columbia basin of Oregon and Washington. Phytopathology 84:1123. (Abstr.)
Hanneman, R.E. 1999. Techniques to transfer germplasm from 2x (1EBN) Mexican species to 2x (2EBN) material via hybridization. Am J Potato Res 76:371. (Abstr.)
Helgeson, J.P., J.D. Pohlman, S. Austin, G.T. Haberlach, S.M. Wielgus, D. Ronis, L. Zambolim, P. Tooley, J.M. McGrath, R.V. James, and W.R. Stevenson. 1998. Somatic hybrids betweenSolanum bulbocastanum and potato: A new source of resistance to late blight. Theor Appl Genet 96:738–742.
Huaman, Z. 1987. Inventory of Andean potato cultivars with resistance to some pests and diseases and other desirable traits.In: International Potato Center Report (CIP), Lima, Peru. pp. 6–22.
Ross, H. 1986. Potato Breeding—Problems and Perspectives. Verlag Paul Parey, Berlin, p. 132.
Singsit, C. and R.E. Hanneman. 1991. Rescuing abortive inter-EBN potato hybrids through double pollination and embryo culture. Plant Cell Rep 9:475478.
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Douches, D.S., Bamberg, J.B., Kirk, W. et al. Evaluation of wildSolanum species for resistance to the US-8 genotype ofPhytophthora infestans utilizing a fine-screening technique. Am. J. Pot Res 78, 159–165 (2001). https://doi.org/10.1007/BF02874771
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DOI: https://doi.org/10.1007/BF02874771