Summary
Embryogenic suspension cultures of the hardwood forest tree yellow-poplar (Liriodendron tulipifera) have the potential to produce millions of plantlets. However, low conversion frequencies limit the realization of this potential. Using 4 embryogenic yellow-poplar lines, we first tested the ability of somatic embryos, selected for their similarity to mature zygotic embryos, to convert to plantlets, then tested physical and chemical treatments for their effects on promoting maturation of somatic embryos and subsequent plantlet production. Embryos selected based on resemblance to mature zygotic embryos and transferred to a hormone-free basal medium without casein hydrolysate (CH) produced plantlets at a frequency of 63%. Populations of synchronized somatic embryos were obtained by repeated fractionation of liquid medium-cultured proembryogenic masses (PEMs) on stainless steel sieves. These fractionated embryos failed to mature properly when cultured in liquid basal medium, however. Development of embryos cultured in basal medium supplemented with 5×10−7 M abscisic acid (ABA) was slowed and embryos appeared to mature properly, with separated cotyledons and little precocious germination. However, ABA-treated embryos only rarely converted to plantlets, possibly due to residual effects of the ABA. PEMs fractionated on sieves, transferred to filter paper and placed on solidified basal medium gave a 60–70% synchronous population of mature embryos 10–12 days following plating. Mature embryos transferred to basal medium without CH converted at a frequency of 72%. The percentage of all embryos differentiating from PEMs on filter paper that formed plantlets was 32%.
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Ammirato, P. V. The effects of abscisic acid on the development of somatic embryos from cells of caraway (Carum carvi L.). Bot. Gaz. 135(4):328–337; 1974.
Becwar, M. R.; Noland, T. L.; Wyckoff, J. L. Maturation, germination, and conversion of Norway spruce (Picea abies L.) somatic embryos to plants. In Vitro Cell. Dev. Biol. 25:575–580; 1989.
Carman, J. G.; Thein ovulo environment and its relevance to cloning wheat via somatic embryogenesis. In Vitro Cell. Dev. Biol. 25:1155–1162; 1989.
Crouch, M. L.; Sussex, I. M. Development and storage protein synthesis inBrassica napus L. embryos in vivo and in vitro. Planta 153:64–74; 1981.
Dunstan, D. I.; Bekkaoui, F.; Pilon, M., et al. Effects of abscisic acid and analogues in the maturation of white spruce (Picea glauca) somatic embryos. Plant Sci. 58:77–84; 1988.
Finkelstein, R.; Crouch, M. L. Rapeseed embryo development in culture on high osmoticum is similar to that in seeds. Plant Physiol. 81:907–912; 1986.
Fujii, J. A.; Slade, D.; Redenbaugh, K. Maturation and greenhouse planting of alfalfa artificial seeds. In Vitro Cell. Dev. Biol. 25:1179–1182; 1989.
Gray, D. J. Quiescence in monocotyledonous and dicotyledonous somatic embryos induced by dehydration., HortScience 22:810–814; 1987.
Gray, D. J. Effects of dehydration and exogenous growth regulators on dormancy, quiescence and germination of grape somatic embryos. In Vitro Cell. Dev. Biol. 25:1173–1178; 1989.
Giuliano, G.; Rosellini, D.; Terzi, M. A new method for purification of the different stages of carrot embryoids. Plant Cell Rep. 2:216–218; 1983.
Hammatt, N.; Davey, M. R. Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean (Glycine max L. Merr.). J. Plant Physiol. 128:219–226; 1987.
McKersie, B. D.; Seneratna, T.; Bowley, S. R., et al. Application of artificial seed technology in the production of hybrid alfalfa (Medicago sativa L.). In Vitro Cell. Dev. Biol. 25:1183–1188; 1989.
Merkle, S. A.; Sommer, H. E. Somatic embryogenesis in tissue cultures ofLiriodendron tulipifera. Can. J. For. Res. 16:420–422; 1986.
Merkle, S. A.; Wetzstein, H. Y.; Sommer, H. E. Somatic embryogenesis in tissue cultures of pecan. HortScience 22:128–130; 1987.
Parrott, W. A.; Dryden, G.; Vogt, S., et al. Optimization of somatic embryogenesis and embryo germination in soybean. In Vitro Cell. Dev. Biol. 24:817–820; 1988.
Preece, J. E.; Zhao, J.; Kung, F. Callus production and somatic embryogenesis from white ash. HortScience 24:377–380; 1989.
Raghavan, V. Embryogenesis in Angiosperms, A Developmental and Experimental Study. Cambridge: Cambridge University Press; 1986:303.
Rajasekaran, K.; Mullins, M. G. Embryos and plantlets from cultured anthers of hybrid grapevines. J. Exp. Bot. 30:399–407; 1979.
Risser, P. G.; White, P. R. Nutritional requirements of spruce tumor cellsin vitro. Physiol. Plant. 15:620–635; 1964.
Snedecor, G. W.; Cochran, W. G. Statistical Methods, Sixth Edition. Ames: Iowa State University Press; 1967:593.
Sommer, H. E.; Brown, C. L. Embryogenesis in tissue cultures of sweetgum. For. Sci. 26:257–260; 1980.
Strickland, S. G.; Nichol, J. W.; McCall, C. M., et al. Effect of carbohydrate source on alfalfa somatic embryogenesis. Plant Sci. 48:113–121; 1987.
Trigiano, R. N.; Beaty, R. M.; Graham, E. T. Somatic embryogenesis from immature embryos of redbud (Cercis canadensis). Plant Cell Rep. 7:148–150; 1988.
Trigiano, R. N.; Beaty, R. M.; Dietrich, J. T. Somatic embryogenesis and plantlet regeneration inCornus florida. Plant Cell Rep. 8:270–273; 1989
Tulecke, W. Somatic embryogenesis in woody perennials. In: Bonga, J. M.; Durzan, D. J., eds. Cell and Tissue Culture in Forestry, Vol. 2. Dordrecht: Martinus Nijhoff Publishers; 1987:61–91.
Tulecke, W.; McGranahan, G. Somatic embryogenesis and plant regeneration from cotyledons of walnut,Juglans regia L. Plant Sci. 40:57–63; 1985.
von Arnold, S.; Hakman, I. Regulation of somatic embryo development inPicea abies, with emphasis on ABA effects. J. Plant Physiol. 132:164–169; 1988.
Wann, S. R. Somatic embryogenesis in woody species. In: Janick, J., ed. Horticultural Reviews, Vol. 10. Portland: Timber Press; 1989:153–181.
Wetzstein, H. Y.; Ault, J. R.; Merkle, S. A. Further characterization of somatic embryogenesis and plantlet regeneration in pecan (Carya illinoensis). Plant Sci. 64:193–201; 1989.
Witham, F. H.; Blaydes, D. F.; Devlin, R. M. Experiments in Plant Physiology. New York: Van Nostrand-Reinhold Co.; 1971:245.
Zieber, N. K.; Brink, R. A.; Graf, L. H., et al. The effect of casein hydrolysate on the growthin vitro of immatureHordeum embryos. Am. J. Bot. 37:144–148; 1950.
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This material is based upon work supported by the U. S. Department of Agriculture Cooperative State Research Service under Agreement No. 85-FSTY-9-0117.
An erratum to this article is available at http://dx.doi.org/10.1007/BF02623699.
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Merkle, S.A., Wiecko, A.T., Sotak, R.J. et al. Maturation and conversion ofLiriodendron tulipifera somatic embryos. In Vitro Cell Dev Biol 26, 1086–1093 (1990). https://doi.org/10.1007/BF02624445
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DOI: https://doi.org/10.1007/BF02624445