Abstract
Atlantic salmon post-smolts were fed diets rich in linoleic acid (sunflower oil, SO), α-linolenic acid (linseed oil, LO) or long-chain polyunsaturated fatty acids (fish oil, FO) for a period of 12 wk. In the liver phospholipids of fish fed SO, the levels of 18∶2n−6, 20∶2n−6, 20∶3n−6 and 20∶4n−6 were significantly elevated compared to both other treatment. In choline phospholipids (CPL), ethanolamine phospholipids (EPL) and phosphatidylserine (PS) the levels of 22∶4n−6 and 22∶5n−6 were significantly elevated in fish fed SO. In liver phospholipids from fish fed LO, 18∶2n−6, 20∶2n−6 and 20∶3n−6 were significantly elevated but 20∶4n−6, 22∶4n−6 and 22∶5n−6 were similar or significantly decreased compared to fish fed FO. Liver phospholipids from fish fed LO had increased 18∶3n−3 and 20∶4n−3 compared to both other treatments while EPL and phosphatidylinositol (PI) also had increased 20∶5n−3. In fish fed LO, 22∶6n−3 was significantly reduced in CPL, PS and PI compared to fish fed FO. Broadly similar changes occurred in gill phospholipids. Production of 12-lipoxygenase metabolites in isolated gill cells stimulated with the Ca2+-ionophore A23187 were significantly reduced in fish fed either SO or LO compared to those fed FO. However, the ratio 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE)/12-hydroxy-5,8,10,14,17-eicosapentaenoic acid (12-HEPE) was significantly elevated in stimulated gill cells from SO-fed fish. Although mean values of thromboxane B2 (TXB2) and prostaglandin E2 (PGE2) were increased in fish fed SO, they were not significantly different from those of the other two treatments.
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Abbreviations
- AA:
-
arachidonic acid
- BHT:
-
butylated hydroxytoluene
- CPL:
-
choline phospholipids
- DHA:
-
docosahexaenoic acid
- EPA:
-
eicosapentaenoic acid
- EPL:
-
ethanolamine phospholipids
- FO:
-
fish oil
- HDHE:
-
hydroxy-4,7,10,13,16, 19-docosahexaenoic acid
- HEPE:
-
hydroxy-5, 8, 10, 14, 17-eicosapentaenoic acid
- HETE:
-
hydroxy-5, 8, 10, 14-eixosatetraenoic acid
- HPLC:
-
high-performance liquid chromatography
- LO:
-
linseed oil
- PGE2 :
-
prostaglandin E2
- PI:
-
phosphatidylinositol
- PS:
-
phosphatidylserine
- PUFA:
-
polyunsaturated fatty acid
- SO:
-
sunfolower oil
- TLC:
-
thin-layer chromatography
- TXB2 :
-
thromboxane B2
- UV:
-
ultraviolet
References
Sprecher, H. (1981)Progr. Lipid Res. 20, 13–22.
Horrobin, D.F. (1983)Rev. Pure Appl. Pharmacol. Sci. 4, 339–386.
Mohrhauer, H., and Holman, R.T. (1963)J. Lipid Res. 4, 151–159.
Garcia, P.T., and Holman, R.T. (1965)J. Am. Oil Chem. Soc. 42, 1137–1141.
Henderson, R.J., and Tocher, D.R. (1987)Prog. Lipid Res. 26, 281–347.
Bell, J.G., Youngson, A., Mitchell, A.I., and Cowey, C.B. (1989)Lipids 24, 240–242.
Weber, P.C. (1990) inAdvances in Prostaglandin, Thromboxane and Leukotriene Research (Samuelsson, B., Dahlen, S-E., Fritsch, J., and Heqvist, P., eds.) Vol. 20, pp. 233–240, Raven Press.
Tocher, D.R., and Sargent, J.R. (1987)Comp. Biochem. Physiol. 87B, 733–739.
Anderson, A.A., Fletcher, T.C., and Smith, G.M. (1981)Comp. Biochem. Physiol. 70C, 195–199.
Terano, T., Salmon, J.A., Higgs, G.A., and Moncada, S. (1986)Biochem. Pharmacol. 35, 779–785.
Budowski, P. (1988) inAspects of Human Nutrition and Dietetics (Bourne, G.H., ed.) Vol. 57, pp. 214–274, Karger, Basel.
Lee, T.H., Mencia-Garcia, J.M., Shih, C., Corey, E.J., Lewis, R.J., and Austen, K.F. (1984)J. Biol. Chem. 259, 2883–2389.
Garg, M., Sebokova, E., Thomson, A.B.R., and Clandinin, M.T. (1988)Biochem. J. 249, 351–356.
Hwang, D.H., Boudreau, M., and Chanmugam, P. (1988)J. Nutr. 118, 427–437.
Olomu, J.M., and Baracos, V.E. (1991)Lipids 26, 743–749.
Lands, W.E.M., Lettellier, P.E., Rome, L.H., and Vanderhoek, J.Y. (1973)Adv. Biosci. 9, 15–28.
Bell, J.G., McVicar, A.H., Park, M.T., and Sargent, J.R. (1991)J. Nutr. 121, 1163–1172.
Bell, J.G., Sargent, J.R., and Raynard, R.S. (1992)Prostaglandins, Leukotrienes and Essentiae Fatty Acids 45, 197–206.
U.S. National Research Council (1981)Nutrient Requirements of Coldwater Fishes, National Academy Pres, Washington, D.C.
Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.
Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.
Vitiello, F., and Zanetta, J.-P. (1978)J. Chromatogr. 166, 637–640.
Christie, W.W. (1982)Lipid Analyses, 2nd edn., pp. 52–53, Pergamon Press, Oxford.
Ackman, R.G. (1980) inAdvances in Fish Science and Technology (Connell, J.J., ed.) pp. 86–103, Fishing News Books Ltd., Farnham.
Moon, T.W., Walsh, P.J., and Mommsen, T.P. (1985)Can. J. Fish Aquat. Sci. 42, 1772–1782.
Powell, W.S. (1982)Methods Enzymol. 86, 467–477.
Brenner, R.R. (1981)Prog. Lipid Res. 20, 41–47.
Marshall, L.A., and Johnston, P.V. (1982)Lipids 17, 905–913.
Sowizral, K.C., Rumsey, G.L., and Kinsella, J.E. (1990)Lipids 25, 246–253.
Whelan, J., Broughton, K.S., and Kinsella, J.E. (1991)Lipids 26, 119–126.
Needleman, P., Raz, A., Minkes, M.S., Ferrendelli, J.A., and Sprecher, H. (1979)Proc. Natl. Acad. Sci. USA 76, 944–948.
German, J.B., Bruckner, G.G., and Kinsella, J.E. (1986)Biochim. Biophys. Acta 875, 12–20.
Buttner, N., Sieglebaum, S.A., and Volterra, A. (1989)Nature 342, 553–555.
Masferrer, J.L., Rios, A.P., and Schwartzman, M.L. (1990)Biochem. Pharmacol. 39, 1971–1974.
Nalbone, G., Grynberg, A., Chevalier, A., Leonardi, A., Termine, E., and Lafont, H. (1990)Lipids 25, 301–306.
Grataroli, R., Leonardi, J., Charbonnier, M., Lafont, R., Lafont, H., and Nalbone, G. (1988)Lipids 23, 666–670.
German, J.B., and Hu, M-L. (1990)Free Rad. Biol. Med. 8, 441–448.
Fischer, S., von Shacky, C., Seiss, W., Strasser, T., and Weber, P.C. (1984)Biochem. Biophys. Res. Commun. 130, 907–918.
Takayama, H., Kroll, M.H., Gimbrone, M.A., and Schafer, A.I. (1989)Biochem. J. 258, 427–434.
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Gordon Bell, J., Dick, J.R. & Sargent, J.R. Effect of diets rich in linoleic or α-linolenic acid on phospholipid fatty acid composition and eicosanoid production in atlantic salmon (Salmo salar). Lipids 28, 819–826 (1993). https://doi.org/10.1007/BF02536236
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DOI: https://doi.org/10.1007/BF02536236