Abstract
Three groups of male rats were fed either a corn oilenriched diet (17%, w/w), a salmon oil-enriched diet (12.5%) supplemented with corn oil (4.5%) or a low-fat diet (4.4%) for eight wk to investigate the possible relationships between dietary fatty acids and lipid composition, and prostaglandin E2 level and phospholipase A2 activity in the rat gastric mucosa.
High-fat diets induced no important variation in total protein, phospholipid and cholesterol contents of gastric mucosa.
Compared with a low-fat diet, corn oil produced a higher n−6/n−3 ratio in mucosal lipids, whereas this ratio was markedly lowered by a fish oil diet.
In comparison with the low-fat diet, the production of prostaglandin E2(PGE2) in gastric mucosa of rats fed salmon oil was significantly, decreased by a factor of 2.8. In the corn oil group, PGE2 production tended to decrease, but not significantly.
In comparison with the low-fat diet, both specific and total gastric mucosal phospholipase A2 activities were increased (+18 and 23%, respectively) in the salmon oil group; they were unchanged in the corn oil group.
It is suggested that the decrease of gastric PGE2 in rats fed fish oil is not provoked by a decrease in phospholipase A2 activity but may be the result of the substitution of arachidonic acid by n−3 PUFA or activation of PGE2 catabolism.
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Abbreviations
- FAME:
-
fatty acid methyl esters
- PA2 :
-
phospholipase A2
- PGE2 :
-
prostaglandin E2
- PGI2 :
-
Prostacycline I2
- PGI3 :
-
Prostacycline I3
- PUFA:
-
polyunsaturated fatty acids
- ANOVA:
-
analysis of variance
- GLC:
-
gas liquid chromatography
- P/S ratio:
-
polyun-saturated to saturated fatty acid ratio
- RIA:
-
radioimmunoassay
References
Robert, A., Schultz, J.R., Nezamis, J.E., and Lancaster, C. (1976)Gastroenterology 70, 359–370.
Konturek, S.J., and Pawlik, W. (1986) inBiological Protection with Prostaglandins (Cohen, M.M., ed.) Vol. II, pp. 45–61, CRC Press Inc., Boca Raton, FL.
Robert, A., Nezamis, J.E., Lancaster, C., and Hanchar, A.J. (1979)Gastroenterology 77, 433–443.
Robert, A. (1984)Gastroenterol. Clin. Biol. 8, 819–827.
Vantrappen, G., Janssens, J., Propiela, T., Kulig, G., Tytgat, G.N.J., Huibregtse, K., Lambert, R., Pauchard, J.P., and Robert, A. (1982)Gastroenterology 83, 357–363.
Wassef, M.K., Lin, Y.N., and Horowitz, M.I. (1978)Biochim. Biophys. Acta 528, 318–330.
Hirohara, J., Sugatani, J., Okumura, T., Sameshima, Y., and Saito, K. (1987)Biochim. Biophys. Acta 919, 231–238.
Grataroli, R., Charbonnier, M., Léonardi, J., Grimaud, J.C., Lafont, H., and Nalbone, G. (1987)Arch. Biochem. Biophys. 258, 77–84.
Goodnight, S.H., Jr., Harris, W.S., Connor, W.E., and Illingworth, D.R. (1982)Arteriosclerosis 2, 87–113.
Dyerberg, J., Bang, H.O., Stofferson, E., Moncada, S., and Vane, J.R. (1978)Lancet 2, 117–119.
Hauge, J.G., and Nicolaysen, R. (1959)Acta Physiol. Scand. 45, 26–30.
Harris, W.S., Connor, W.E., and McMurry, M.P. (1983)Metabolism 32, 179–184.
Kromhout, D., Bosshieter, E.B., and Coulander, C.L. (1985)N. Eng. J. Med. 312, 1205–1209.
Durand, G., Pascal, G., Legrand, P., and Gounelle de Pontanel, H. (1985)Med. Nutr. 21, 391–406.
Iritani, N., and Fujikawa, S. (1982)J. Nutr. Sci. Vitaminol. 28, 621–629.
Lokesh, B.R., Hsieh, H.L. and Kinsella, J.E. (1986)Ann. Nutr. Metab. 30, 357–364
Croft, K.D., Beilin, L.J., Vandongen, R., and Mathews, E. (1984)Biochim. Biophys. Acta 795, 196–207.
Bradford, M.M. (1976)Anal. Biochem. 72, 248–254.
Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.
Amic, J., Lairon, D., and Hauton, J.C. (1972)Clin. Chim. Acta 40, 107–114.
Cecchi, G., Biasini, S., and Castano, J. (1985)Rev. Fr. Corps Gras 32, 163–164.
Léonardi, J., Termine, E., Morand, F., Lafont, R., Portugal, H., Lafont, H., and Nalbone, G. (1987)Lipids 22, 517–522.
Galli, C., Agradi, E., Petroni, A., and Tremoli, E. (1981)Lipids 16, 165–172.
McGregor, L., Morazain, R., and Renaud, S. (1980)Lab. Invest. 43, 438–442.
Swanson, J.E., and Kinsella, J.E. (1986)J. Nutr. 116, 514–523.
Gudbjarnason, S., and Oskarsdottir, G. (1977)Biochim. Biophys. Acta 487, 10–15.
Sprecher, H. (1981)Progr. Lipid Res. 20, 13–22.
Brenner, R.R. (1982)Progr. Lipid Res. 20, 41–47.
Konturek, S.J., Piastucki, I., Brzozowski, T., Radecki, T., Dembinska-Kiec, A., and Griglewski, R. (1981)Gastroenterology 80, 4–9.
de Deckere, E.A.M., Nugteren, D.H., and Ten Hoor, F. (1979)Prostaglandins 17, 947–955.
Spector, A.A., Kaduce, T.L., Hoak, J.C., and Fry, G.L. (1981)J. Clin. Invest. 68, 1003–1011.
Spector, A.A., Hoak, J.C., Fry, G.L., Denning, G.H., Stoll, L.L., and Smith, J.B. (1980)J. Clin. Invest. 65, 1003–1012.
Culp, B.R., Titus, B.G., and Lands, W.E.M. (1979)Prostaglandins Med. 3, 269–278.
Corey, E.J., Shih, C., and Cashman, J.R. (1983)Proc. Natl. Acad. Sci. USA 80, 3581–3584.
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Grataroli, R., Léonardi, J., Charbonnier, M. et al. Effects of dietary corn oil and salmon oil on lipids and prostaglandin E2 in rat gastric mucosa. Lipids 23, 666–670 (1988). https://doi.org/10.1007/BF02535665
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DOI: https://doi.org/10.1007/BF02535665