Abstract
WhereasSaccharomyces cerevisiae when grown in continuous culture under anaerobic conditions requires ergosterol,Schizosaccharomyces japonicus (syn.Sch. versatilis) can grow without this substance; sporulation is under anaerobic conditions hardly less prolific than under aerobic conditions.
The ergosterol content of anaerobically grown cells ofSch. japonicus was only 0.01 %, that of aerobically grown cells 0.24%. Under anaerobic conditions, cell growth was hardly affected by the polyene antibiotic pimaricin; under aerobic conditions,Sch. japonicus is about as sensitive asS. cerevisiae. Qualitatively, the antagonistic effect of ergosterol on pimaricin action is the same inSch. japonicus and inS. cerevisiae.
The relation between ergosterol content and the polyene sensitivity strongly confirms existing views (see Kinsky, 1967) on the mechanism of action of polyene antibiotics.
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The skilful technical assistance of Miss Marry Reinink is gratefully acknowledged.
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Bulder, C.J.E.A. Anaerobic growth, ergosterol content and sensitivity to a polyene antibiotic, of the yeastSchizosaccharomyces japonicus . Antonie van Leeuwenhoek 37, 353–358 (1971). https://doi.org/10.1007/BF02218505
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DOI: https://doi.org/10.1007/BF02218505