Abstract
Production of extracellular lipase byRhodotorula glutinis was substantially enhanced when the type and concentration of carbon and nitrogen source, the initial pH of culture medium and the growth temperature were consecutively optimized. Lipase activity as high as 30.4 U/ml of culture medium was obtained at optimum conditions, comparing favourably with most of the activities reported for other lipase hyperproducing microorganisms. The enzyme was optimally active at pH 7.5 and 35°C and had, at optimum pH, half-lives of 45 and 11.8 min at 45 and 55°C respectively. The high activity and kinetic characteristics of the enzyme make this process worthy of further investigation.
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References
Anderson, M.M. and McCarty, E. (1972).Anal. Biochem. 45, 260–270.
Baillargeon, M.W., Bistline, R.G. and Sonnet, P.E. (1989).Appl. Microbiol. Biotechnol. 30, 92–96.
Chopra, A.K. and Chander, H. (1983).J. Appl. Bacter. 54, 163–169.
Chader, H. and Klostermeyer, H. (1983).Milchwissenschaft 38, 410–412.
Chader, H., Batish, V.K., Sannabhadti, S.S. and Srinivasan, R.A. (1980).J. Food Sci. 45, 598–600.
Eitenmiller, R.R., Vakil, J.R. and Shahani, K.M. (1970).J. Food Sci. 35, 130–133.
Espinosa, E., Sanchez, S. and Farres, A. (1990).Biotechnol. Lett. 12, 209–214.
Hegedus, D.D. and Khachatourians, G.G. (1988).Biotechnol. Lett. 10, 637–642.
Ibrahim, C.O., Hayashi, M. and Nagai, S. (1987).Agr. Biol. Chem. 51, 37–45.
Iwai, M., Okumura, S. and Tsujisaka, Y. (1980).Agr. Biol. Chem. 44, 2731–2732.
Langrand, G., Rondot, N., Triantaphylides, C. and Baratti, J. (1990).Biotechnol. Lett. 12, 581–586.
Lobyreva, L.B., Yee, Y.A., Marchenkova, E.R. and Umanskii, H.S. (1978). U.S.S.R. patent 608835.
Muderhwa, J.M., Ratomahenina, R., Graille, J. and Galzy, P. (1985).JAOCS 62, 1031–1036.
Muraoka, T., Ando, T. and Okuda, H. (1982).J. Biochem. 92, 1933–1939.
Nahas, E. (1988).J. Gen. Microbiol. 134, 227–233.
Nakashima, T., Fukuda, H., Kyotani, S. and Morikawa, H. (1988).J. Ferment. Technol. 66, 441–446.
Nishio, T., Chikano, T. and Kamimura, M. (1986).Eur. Pat. Appl. EP 204, 24.
Okeke, C.N. and Okolo, B.N. (1990).Biotechnol. Lett. 92, 747–750.
Petrovic, S.E., Skrinjar, M., Becarevic, A., Vujicic, I.F. and Banka, L. (1990).Biotechnol. Lett. 12, 299–304.
Pulley, J.E. (1969).Food Eng. 14, 68–71
Seitz, E.W. (1974).J. Am. Oil Chem. Soc. 51, 12–16.
Stern, A.M., Ordal, Z.J. and Halvorson, H.O. (1954).J. Bacter. 68, 24–27.
Sugiura, M., Oikawa, T., Hirano, K. and Inukai, T. (1977).Bioch. Bioph. Acta 488, 353–358.
Suzuki, M., Yamamoto, H. and Mizugaki, M. (1986).J. Biochem. 100, 1207–1213.
Sztajer, H. and Maliszewska, I. (1989).Biotechnol. Lett. 11, 895–898.
Tanabe Seiyaku Zrd. (1970). Japanese patent 3187/170
Tsujisaka, Y., Iwai, M. and Tominaga, Y. (1973).Agr. Biol. Chem. 37, 1457–1464.
Valero, F., Fukuda, H., Kyotani, S. and Morikawa, H. (1988).J. Ferment. Technol. 66, 441–446.
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Papaparaskevas, D., Christakopoulos, P., Kekos, D. et al. Optimizing production of extracellular lipase fromRhodotorula glutinis . Biotechnol Lett 14, 397–402 (1992). https://doi.org/10.1007/BF01021254
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DOI: https://doi.org/10.1007/BF01021254