Abstract
The synthesis of amylolytic enzymes by the maltose not-utilizing Trichoderma viride strain CBS 354.44 requires the presence of starch or dextrins. Several readily utilizable carbon sources such as glucose and glutamic acid were shown to exert a strong catabolite repression which completely inhibited enzyme induction by starch or dextrins.
Enzyme synthesis occurs in the exponential and in the stationary growth phase. In the latter, the ratio between saccharifying and dextrinizing enzyme activity is invariably high. In the exponential growth phase this ratio depends on the nature of the inducing substrate. Growth on starch results in an initially high production of dextrinizing activity, the saccharifying one becoming predominant in the course of exponential growth. The latter activity in dextrin DE 30 cultures is predominant from the very beginning. Thus, the amylolytic enzyme system of T. viride consists of at least two different enzymes, the synthesis of each being controlled specifically. The careful regulation of the synthesis of the dextrinizing enzyme is discussed with special reference to the production of non-utilizable maltose by the latter.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Aube, C. and Gagnon, C. 1969. Effect of carbon and nitrogen nutrition of Trichoderma Pers. ex Pries. — Can. J. Microbiol. 15: 703–706.
Barton, L. L., Lineback, D. R. and Georgi, C. E. 1969. The influence of nitrogen and carbon sources on the production of glucoamylase by Aspergilli. — J. Gen. Appl. Microbiol. 15: 327–344.
Barton, L. L., Georgi, C. E. and Lineback, D. R. 1972. Effect of maltose on glucoamylase formation by Aspergillus niger. — J. Bacteriol. 111: 771–777.
Danielson, R. M. and Davey, C. B. 1973. Carbon and nitrogen nutrition of Trichoderma. — Soil Biol. Biochem. 5: 505–515.
Magasanik, B. 1961. Catabolite repression. — Cold Spring Harbor Symp. Quant. Biol. 26: 249–256.
Schellart, J. A. 1975. Fungal protein from corn waste effluents. — Meded. Landbouwhogeschool Wageningen, 75-17: 1–106.
Schellart, J. A., Visser, F. M. W., Zandstra, T. and Middelhoven, W. J. 1976. Starch degradation by the mould Trichoderma viride. I. The mechanism of starch degradation. —Antonie van Leeuwenhoek 42: 229–238.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schellart, J.A., van Arem, E.J.F., van Boekel, M.A.J.S. et al. Starch degradation by the mould Trichoderma viride II. Regulation of enzyme synthesis. Antonie van Leeuwenhoek 42, 239–244 (1976). https://doi.org/10.1007/BF00394120
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00394120