Abstract
We studied the effects of different sources of carbon, nitrogen, and dietary elements on the biosynthesis of amylase inhibitor produced by two strains from the Russian National Collection of Industrial Microorganisms: Streptomyces violaceus (code: Ac-1734) and Streptomyces lucensis (Ac-1743). We found that the biosynthesis of amylase inhibitor can be regulated by limiting carbon and oxygen concentrations, maintaining constant a C: N ratio and a stable proportion of carbohydrates (dextrins, maltose and glucose) in the medium, and by adding an extra organic source of nitrogen to the medium. The inhibitors produced by the cultures are pseudo-polysaccharides.
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Abbreviations
- AA:
-
amylolytic activity
- IU:
-
inhibitory unit
- CMC:
-
carboxymethylcellulolytic activity
- MW:
-
molecular weight
- DE:
-
dextrose equivalent
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Original Russian Text © N.Yu. Sharova, 2015, published in Biotekhnologiya, 2015, No. 5, pp. 22–33.
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Sharova, N.Y. Biosynthesis of amylase inhibitor by Streptomycete cultures. Appl Biochem Microbiol 52, 723–732 (2016). https://doi.org/10.1134/S000368381608007X
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DOI: https://doi.org/10.1134/S000368381608007X