Abstract
Invertase produced by a strain of Aspergillus niger showed the following main characteristics: maximum activity at 60°C, pH 5.0; K m with sucrose as substrate, 0.0625mm; V max 0.013 mol/min; and free energy 9132 cal/mol. The metal ions and p-chloromercuribenzoate (PCMB) acted as inhibitors respectively.
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Literature Cited
Dox AW (1910) The intracellular enzymes of Penicillum and Aspergillus. US Dept Agric Bur Anim Ind Bull: 120–121
Ettalibi M, Baratti JC (1987) Purification, properties and comparison of invertase, exoinulinases and endoinulinases of Aspergillus ficuum. Appl Microbiol Biotechnol 26:13–20
Gascon S, Neumann NP, Lampen JO (1968) Comparative study of the properties of the purified internal and external invertase from yeast. J Biochem 243:1573–1577
Hayashi S, Matsuzaki K, Takasaki Y, Ueno H, Imada K (1992a) Purification and properties of β-fructo-furanosidase from Aspergillus japonicus. World J Microbiol Biotechnol 8:276–279
Hayashi S, Matsuzaki K, Takasaki Y, Ueno H, Imada K (1992b) Production of β-fructofuranosidase by Aspergillus japonicus. World J Microbiol Biotechnol 8:155–159
Hirayama M, Sumi N, Hidaka H (1989) Purification and properties of fructooligosaccharide producing β-fructofuranosidase from Aspergillus niger ATCC 0611 Agric Biol Chem 53:667–673
Hoshino J, Momose A (1966) Comparative studies on the two forms of yeast invertase. J Gen Appl Microbiol 12:163–177
Klein RD, Deibel MR, Sarcich JL (1989) Purification and characterization of invertase from a novel industrial yeast. Schwanniomyces occidentalis. Prep Biochem 19:293–319
Lowry O, Rosebrough N, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275
Maruyama Y, Onodera K (1979) Production and some properties of invertase isozymes of Fusarium oxysporum. J Gen Appl Microbiol 25:361–366
Metzenberg RL (1964) Enzymically active subunits of Neurospora invertase. Biochim Biophys Acta 89:291–302
Nelson N (1944) A photometric adaption of the Somogy method for the determination of glucose. J Biol Chem 153:375–380
Nishizawa M, Maruyama Y, Nakamura M (1980) Purification and characterization of invertase isozymes from Fusarium oxysporum. Agric Biol Chem 44:489–498
Somogy M (1952) Notes on sugar determination. J Biol Chem 195:19–23
Trevithick JR, Metzenberg RL (1966) Molecular sieving by Neurospora cell walls during secretion of invertase isoenzymes. J Bacteriol 92:1010–1015
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Rubio, M.C., Maldonado, M.C. Purification and characterization of invertase from Aspergillus niger . Current Microbiology 31, 80–83 (1995). https://doi.org/10.1007/BF00294280
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DOI: https://doi.org/10.1007/BF00294280