Summary
We report the isolation and regeneration of protoplasts from an embryogenic banana (Musa spp.) cell suspension culture initiated from in vitro proliferating meristems. A high yielding isolation method (up to 6×107 protoplasts.ml−1 packed cells) is discussed. Optimal regeneration, with more than 50% of the protoplasts showing initial cell division, occurred when high inoculation densities (106 protoplasts.ml−1) or nurse cultures were applied. Under these conditions, the frequency of microcolony formation was 20–40%. These microcolonies developed directly, without an intervening callus phase, into somatic embryos which later germinated and formed plantlets.
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Panis, B., Van Wauwe, A. & Swennen, R. Plant regeneration through direct somatic embryogenesis from protoplasts of banana (Musa spp.). Plant Cell Reports 12, 403–407 (1993). https://doi.org/10.1007/BF00234701
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DOI: https://doi.org/10.1007/BF00234701