Abstract
Somatic embryos induced from callus cultures of lemongrass [Cymbopogon flexuosus (Nees) Wats.] on Murashige and Skoog medium supplemented with 5 mg/l of 2,4-D, 0.1 mg/l of NAA and 0.5 mg/l of Kn developed into plantlets when plated on a medium supplemented with 3 mg/l of BA, 1 mg/l of GA3 and 0.1 mg/l of NAA. The regeneration potential of callus was retained for more than 2 years on the nutrient medium supplemented with comparatively lower levels of growth regulators (2,4-D at 2 mg/l, NAA at 0.1 mg/l and Kn at 0.25 mg/l). Approximately 30–35 plantlets were produced after two months of culture per 100 mg of callus inoculated. Regenerants were transplanted into soil and transferred to the field for assessment of various morphological and biochemical characteristics. The results of 1 year of field trials showed that plants derived from somatic embryoids were more uniform in all the characteristics examined when compared with the field performance of plants raised through slips by standard propagation procedures. Thus, a procedure has been developed for high frequency long term plant production of lemongrass through in vitro methods.
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Abbreviations
- 2,4-D:
-
2,4 -dichlorophenoxyacetic acid
- NAA:
-
α -naphthalene acetic acid
- Kn:
-
kinetin
- BA:
-
benzyladenine
- GA3 :
-
gibberllic acid
- MS:
-
Murashige and Skoog (1962) basal medium
References
Ammirato PV (1989) In: Handbook of plant cell culture, vol 1. Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) MacMillan, New York, pp 82–123
Baruah A, Bordoloi DN (1989) Plant Cell Reports 8: 483–485
Chu CC, Wang C, Sun C (1975) Scientia Sinica 18: 659–668
Guenther F (1950) In: The essential oils, vol 4, nD. Van Nostrand Company, Inc, New York, pp 20–63
Hatano K, Kamura K, Shoyama Y, Nishioka I (1988) Planta Medica 54: 152–154
Hiraoka N, Kodama T, Oyanogi M, Nakano S, Tomita Y, Yamada N, Lida O, Satake M (1986) Plant Cell Reports 5: 319–321
Husain A (1991) In: Newer trends in essential oils and flavours. Dhar KL, Thappa RK, Agarwal SG (eds) Tata McGraw-Hill Publishing Co. Ltd., New Delhi, pp 174–190
Mathur AK, Ahuja PS, Pandey B, Kukreja AK, Mandal S (1988) Plant Breeding 101: 321–334
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Nayak S, Debata BK, Sahoo S (1992) Orissa J Hort 20: 47–50
Patra NK, Srivastava RK, Chauhan SP, Ahmad A, Misra LN (1990) Planta Medica 56: 239–240
Pillay KP (1961) Lemongrass in India, Farm Bull. No. 61. Ministry of Food and Agriculture, New Delhi, pp 1–26
Rao BL, Sobti SN, Sarin YK, Kaul BL (1991) In: Newer trends inessential oils and flavours. Dhar KL, Thappa RK, Agarwal SG (eds) Tata McGraw-Hill Publishing Co. Ltd., New Delhi, pp 322–326
Robbins SRJ (1983) Selected markets for the essential oils of Lemongrass, Citronella and Eucalyptus. Trop Prod Inst, London.
Sharp WR, Evans DA, Sondahl MR (1982) In: Plant tissue culture, Proc. 5th Internat. Cong Plant Tissue Culture. Fujiwara, A (ed) Japan,pp 759–762
Shoyama Y, Kamura K, Nishioka I (1988) Planta Medica 54: 155–156
Sreenath HL, Jagadishchandra KS (1991) In: Biotechnology in agriculture and forestry, vol 15, medicinal and aromatic plants III, Bajaj YPS (ed), Springer-Verlag, Berlin, pp 211–236
Vasil IK (1982) In: Cell culture and somatic cell genetics of plants, vol 1. Vasil IK (ed). Academic Press, Inc, Orlando, Florida, pp 36–41
Vasil IK (1986) J Plant Physiol 128: 193–218
Verma V, Rao PG, Zutshi V, Sobti SN (1985) Indian Drugs 22: 291–293
Vogel Al (1986) In: Textbook of practical organic chemistry, English language Book Society, Longman Group Ltd., England, pp 143–146
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Communicated by G. C. Phillips
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Nayak, S., Debata, B.K. & Sahoo, S. Rapid propagation of lemongrass (Cymbopogon flexuosus (Nees) Wats.) through somatic embryogenesis in vitro . Plant Cell Reports 15, 367–370 (1996). https://doi.org/10.1007/BF00232373
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DOI: https://doi.org/10.1007/BF00232373