Abstract
Polyclonal antisera have been used for the development of a novel serotaxonomic system for methanogenic bacteria by quantifying immunological cross-reactions of the antisera with heterologous strains. This system corresponds well to phylogenetic relationships among methanogenic bacteria as analysed by other authors, and is in good agreement with the existing immunological fingerprint for immunotyping of methanogenic bacteria.
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Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS (1979) Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296
Bryniok D, Trösch W (1989) ELISA techniques for the determination of methanogenic bacteria. Appl Microbiol Biotechnol 32:235–242
Conway de Macario E, Macario AJL (1986) Immunology of Archaebacteria: identification, antigenic relationships and immunochemistry of surface structures. Syst Appl Microbiol 7:320–324
Conway de Macario E, Macario AJL, Wolin MJ (1982a) Specific antisera and immunological procedures for characterization of methanogenic bacteria. J Bacteriol 149:320–328
Conway de Macario E, Wolin MJ, Macario AJL (1982b) Antibody analysis of relationships among methanogenic bacteria. J Bacteriol 149:316–319
Conway de Macario E, Macario AJL, Jovell RJ (1983) Quantitative slide microimmunoenzymetic assay (Micro-SIA) for antibodies to particulate and non-particulate antigens. J Immunol Methods 59:39–47
Dudley RA, Edwards P, Ekins RP, Finney DJ, McKenzie IGM, Raab GM, Rodboard D, Rodgers RPC (1985) Guidelines for immunoassay data processing. Clin Chem 31:1264–1271
Hungate RE (1969) A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132
Jones WJ, Nagle DP, Whitman WB (1987) Methanogens and the diversity of arachaebacteria. Microbiol Rev 51:135–177
Macario AJL, Conway de Macario E (1983) Antigenic fingerprinting of methanogenic bacteria with polyclonal antibody probes. Syst Appl Microbiol 4:451–458
Miller TL, Wolin MJ (1974) A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol 27:985–987
Sowers KR, Johnson JL, Ferry JG (1984) Phylogenetic relationships among the methylotrophic methane-producing bacteria and emendation of the family Methanosarcinaceae. Int J Syst Bacteriol 34:444–450
Tu J, Prangishvilli D, Huber H, Wildgruber G, Zillig W, Stetter KO (1982) Taxonomic relations between Archaebacteria including 6 novel genera examined by cross hybridizations of DNAs and 16S rRNAs. J Mol Evol 18:109–114
Winkler G, Heinz FX, Kunz C (1986) A personal computer program using a 4-component logit for the evaluation of ELISA calibration curves with unknown asymptotic values. Comput Method Program Biomed 22:167–170
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Bryniok, D., Trösch, W. Taxonomy of methanogens by ELISA techniques. Appl Microbiol Biotechnol 32, 243–247 (1989). https://doi.org/10.1007/BF00165894
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DOI: https://doi.org/10.1007/BF00165894