Abstract
A method was developed for the in vitro clonal propagation of shoots from a range of wild rice and other grass species that have important genetic traits such as drought resistance and salinity tolerance. The axenic multiple shoot cultures, which were suitable for DNA and protein extraction or direct protoplast isolation, could be maintained without subculture for between 2 and 3 months or rapidly multiplied for the subsequent production of mature plants and seeds. Basal sections of the micropropagated shoots also provided novel explants for the production of highly embryogenic callus, from some species, that could be regenerated into green plants. It is envisaged that this clonal propagation technique could aid the genetic manipulation of cultivated rice by providing a means to vegetatively conserve valuable genetic resources, a technique to rapidly multiply novel hybrid material and a source of embryogenic callus that will allow the application of biotechnological techniques, such as somatic hybridization and genetic transformation, to previously unexploited species.
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Abbreviations
- BA:
-
N6-benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
α-naphthaleneacetic acid
- PAR:
-
photosynthetically-active radiation
References
Abdullah R, Thompson JA & Cocking EC (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4: 1087–1090
Akagi H, Sakamoto M, Negishi T & Fujimura T (1989) Construction of rice cybrid plants. Mol. Gen. Genet. 215: 501–506
Baset A, Finch RP & Cocking EC (1991) Plant regeneration from protoplasts of the wild rice species, Oryza rufipogon. Plant Cell Rep 10: 200–203
Conger BV, Hanning GE, Gray DJ & McDaniel JK (1983) Direct embryogenesis from mesophyll cells of orchardgrass. Science 221: 850–851
Conger BV, Novak FJ, Afza R & Erdelesky K (1987) Somatic embryogenesis from cultured leaf segments of Zea mays. Plant Cell Rep. 6: 345–347
Farooq S & Mujtaba Naqvi SH (1987) Problems and prospects of rice x kallar grass hybridization. Pakistan J. Sci. Ind. Res. 30: 660–663
Finch RP, Slamet IH & Cocking EC (1990) Production of heterokaryons by the fusion of mesophyll protoplasts of Porteresia coarctata and cell suspension-derived protoplasts of Oryza sativa: A new approach to somatic hybridization in rice. J. Plant Physiol. 136: 592–598
Finch RP, Lynch PT, Jotham JP & Cocking EC (1991) The isolation, culture and fusion of rice protoplasts. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 14 (pp 251–268). Springer-Verlag, Heidelberg
Greco B, Lomonaca A, Boggini B, Tomasini C & Tanzarella OA (1990) Clonal propagation of rice through proliferation of axillary shoots. Euphytica 48: 123–127
Hayashi Y, Kyozuka J & Shimamoto K (1988) Hybrids of rice (Oryza sativa L.) and wild Oryza species obtained by cell fusion. Mol. Gen. Genet. 214: 6–10
Jena KK & Khush GS (1985) Production of monosomic alien addition lines of O. sativa having a single chromosome of O. officinalis. Proc. 1st Intl. Rice Genetics Symp. IRRI, Philippines
Jena KK & Khush GS (1990) Introgression of genes from Oryza officinalis Well ex. Watt. to cultivated rice, Oryza sativa L. Theor Appl. Genet. 80: 737–745
Khush GS, Ling KC, Aquino RC & Aguiero VM (1977) Breeding for resistance to grassy stunt in rice. Proc 3rd Intl. Cong. SABRAO. Canberra, Australia. Plant Breeding Papers 1–4 (6): 3–9
Kumari DS, Sharma NP & Rao GJN (1988) Micropropagation of cytosterile rice stocks. Intl. Rice Res. Newsl. 13: 5–6
Kyozuka J, Kaneda T & Shimamoto K (1989) Production of cytoplasmic male sterile rice (Oryza sativa L.) by cell fusion. Bio/Technology 7: 1171–1174
Linsmaier EM & Skoog F (1962) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100–127
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Sitch LA, Dalmacio RD, Elloran E, Romero GO, Amante AD, Leung H, Nelson R & Khush GS (1990) Wide hybridization for rice improvement (abstract). Proceedings 4th annual meeting of the Rockefeller Foundation's International Programme on Rice Biotechnology. IRRI. Philippines. May 9–12.
Terada R, Kyozuka J, Nishibayashi S & Shimamoto JK (1987) Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing). Mol. Gen. Genet. 210:39–43
Thompson JA, Abdullah R & Cocking EC (1986) Protoplast culture of rice (Oryza sativa L.) using media solidified with agarose. Plant Sci. 47: 123–133
Wang M-S, Zapata FJ & de Castro DC (1987) Plant regeneration through somatic embryogenesis from mature and young inflorescence of wild rice (Oryza perennis Moench). Plant Cell Rep. 6: 294–296
Yang Z-O, Shikanai T, Mori K & Yamada Y (1989) Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.) Theor. Appl. Genet. 77: 305–310
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Finch, R.P., Baset, A., Slamet, I.H. et al. In vitro shoot culture of wild Oryzae and other grass species. Plant Cell Tiss Organ Cult 30, 31–39 (1992). https://doi.org/10.1007/BF00039998
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DOI: https://doi.org/10.1007/BF00039998