Summary
We obtained cybrid plants by electrofusing γ-irradiated protoplasts of a cytoplasmic male-sterile line “A-58 CMS” (Oryza sativa L.) and iodoacetamide (IOA)-treated protoplasts of the fertile (normal) rice cultivar “Fujiminori”. The cybridity of the plants was confirmed by mitochondrial (mt) DNA restriction endonuclease, and plasmid-like DNA analyses, and by isozyme, cytological and morphological investigations. The chromosome number of the cybrid plants is 24.
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References
Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4:1087–1090
Aviv D, Galun E (1980) Restoration of fertility in cytoplasmic male sterile (CMS) Nicotiana sylvestris by fusion with X-irradiated N. tabacum protoplasts. Theor Appl Genet 58:121–127
Coulibaly MY, Demarly Y (1986) Regeneration of plantlets from protoplasts of rice, Oryza sativa L. Z Pflanzenzücht 96:79–81
Endo T, Komiya T, Masumitsu Y, Morikawa H, Yamada Y (1987) An intergeneric hybrid cell line of Duboisia hopwoodii and Nicotiana tabacum by protoplast fusion. J Plant Physiol 129:453–459
Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Cult Lett 2:74–75
Galun E, Arzee-Genen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization in Nicotiana: Mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions. Mol Gen Genet 186:50–56
Heyser JW, Dykes TA, Demott KJ, Nabors MW (1983) High frequency, long term regeneration of rice from callus culture. Plant Sci Lett 29:175–182
Kothari SL, Monte DC, Widholm JM (1986) Selection of Daucus carota somatic hybrids using drug resistance markers and characterization of their mitochondrial genomes. Theor Appl Genet 72:494–502
Kurata N, Iwata N, Omura T (1981) Karyotype analysis in rice II. Identification of extra chromosomes in trisomic plants and banding structure on some chromosomes. Jpn J Genet 56:41–50
Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206:408–413
Menczel L, Nagy F, Lazar G, Maliga P (1983) Transfer of cytoplasmic male sterility by selection for streptomycin resistance after protoplast fusion in Nicotiana. Mol Gen Genet 189:365–369
Menczel L, Morgan A, Brown S, Maliga P (1987) Fusion-mediated combination of Ogura-type cytoplasmic male sterility with Brassica napus plastid using X-irradiated CMS protoplasts. Plant Cell Rep 6:98–101
Ozias-Akins P, Pring DR, Vasil IK (1987) Rearrangements in the mitochondrial genome of somatic hybrid cell lines of Pennisetum americamum (L.) K. Schum. + Panicum maximum Jacq. Theor Appl Genet 74:15–20
Shikanai T, Yang ZQ, Yamada Y (1987) Properties of the circular plasmid-like DNA B1 from mitochondria of cytoplasmic male-sterile rice. Plant Cell Physiol 28:1243–1251
Sidorov VA, Menczel L, Nagy F, Maliga P (1981) Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate-treated protoplasts. Planta 152:341–345
Smith SM, Street HE (1974) The decline of embryogenic potential as callus and suspension cultures of carrot (Daucus carota L.) are serially subcultured. Ann Bot 38:223–241
Terada R, Kyozuka J, Nishibayashi S, Shimamoto K (1987) Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing). Mol Gen Genet 210:39–43
Toriyama K, Hinata K, Sasaki T (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor Appl Genet 73:16–19
Vries SE de, Jacobsen E, Jones MGK, Loonen AEHM, Tempelaar MJ, Wijbrandi J, Freenstra WJ (1987) Somatic hybridization of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion. Theor Appl Genet 73:451–458
Yamada Y, Yang ZQ, Tang DT (1985) Regeneration of rice plants from protoplasts. Rice Genet Newslett 2:94–95
Yamada Y, Yang ZQ, Tang DT (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep 4:85–88
Yang ZQ, Shikanai T, Yamada Y (1988) Asymmetric hybridization between cytoplasmic male-sterile (CMS) and fertile rice (Oryza sativa L.) protoplasts. Theor Appl Genet 76:801–808
Zelcer A, Aviv D, Galun E (1978) Interspecific transfer of cytoplasmic male sterility by fusion between protoplasts of normal Nicotiana sylvestris and X-ray irradiated protoplasts of male-sterile N. tabacum. Z Pflanzenphysiol 90:397–407
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Yang, Z.Q., Shikanai, T., Mori, K. et al. Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.). Theoret. Appl. Genetics 77, 305–310 (1989). https://doi.org/10.1007/BF00305820
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DOI: https://doi.org/10.1007/BF00305820