Abstract
Acoustic signals are used for intraspecific communication in bark beetles and a variety of stridulatory mechanisms have evolved within the subfamily Scolytinae (Coleoptera: Curculionidae). Bark beetles use stridulatory signals for communication at the tree surface and within tunnels inside tree tissues. Bark beetles produce a variety of call types that are broadband with frequencies ranging from 1 to 80 kHz. Not only are airborne and substrate-borne vibrations available, but every stridulation event produces both airborne and substrate-borne vibrations via the same action of the animal. Vibrations appear to be used during species recognition, premating interactions, pair formation, mate selection, intraspecies aggression, territoriality, and predator deterrence. No sound receptors have been located for bark beetles; however, we propose potential locations in this chapter. We provide an overview of acoustic communication and its use by adult bark beetles, describe their stridulatory structures, interpret how vibrations move within tree materials and how this affects the beetles’ ecology and behavior, and present technical and applied applications of acoustic tools for bark beetle management.
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1 Introduction
Bark beetles (Coleoptera: Curculionidae: Scolytinae) are an ecologically and economically important subfamily of weevils that colonize tree phloem and other plant parts such as seeds and stems (Raffa et al. 2015). Over 6000 species of Scolytinae have been described (Hulcr et al. 2015) and can be found throughout the world wherever trees are located (Vega and Hofstetter 2015). Several bark beetle species are capable of altering large scale forest patterns over time via their selection of particular trees and resulting tree mortality (Eidmann 1992; Raffa et al. 2008; Bentz et al. 2010). Most bark beetle species utilize tree materials where they mate, lay eggs, and complete larval development (Raffa et al. 2015). Life history strategies can be monogamous or polygamous with regard to mating, and solitary or gregarious with regard to feeding (Kirkendall 1983; Wermelinger 2004). Bark beetles are highly adapted to life within trees and thus are adapted to boring and communicating within tree material. Adult beetles bore into phloem making cylindrical tunnels that often lack light (i.e., complete darkness) and may contain high levels of plant defensive compounds such as monoterpenes (Raffa et al. 1993). Signals from air- and substrate-borne vibrations become important once beetles are within tunnels or at the tunnel entrance (Rudinsky and Michael 1972, 1973; Ryker and Rudinsky 1976a; Swaby and Rudinsky 1976; Rudinsky et al. 1978 and many others).
Host tree selection and mate location involve chemical cues via gustation (e.g., tasting tree tissues), olfaction (e.g., detection of pheromones related to beetles within tree tissues), and possibly sound (Rudinsky and Michael 1974; Ryker 1984; Raffa et al. 2015). Acoustic cues have been proposed for host tree location (Mattson and Haack 1987), and some evidence suggests that they can hear and respond to the sounds resulting from tree cavitation in response to drought conditions (Kaiser 2014). Multimodal communication, such as chemical and acoustic are likely required to convey species-specific and individual information to conspecifics (typically of the opposite sex) within the tree (Rudinsky and Michael 1972; Rudinsky et al. 1973; Ryker et al. 1979; Birch 1984).
Bark beetles produce sound by rubbing body parts or body-substrate friction (Barr 1969; Lyal and King 1996) called stridulations (Wessel 2006). There is great diversity in the types of structures employed in stridulation by bark beetles among genera, species and between the sexes (Fig. 21.1) (Barr 1969; Ryker 1988; Lyal and King 1996; Kerchev 2015). Interestingly, stridulatory structures, regardless of the location of the body, are often sexually dimorphic and less-developed or absent in the sex that initiates tunnel construction (i.e., pioneer or colonizing sex) (Barr 1969).
Bark beetles produce a variety of call types that vary in temporal characteristics. General call types appear somewhat consistent within genera (Rudinsky and Michael 1974; Lyal and King 1996; Yturralde 2013); however, intraspecific differences occur between chirps produced in different contexts (Michael and Rudinsky 1972; Fleming et al. 2013). Signal characteristics in bark beetles show that both air- and substrate-borne vibrations are present and potentially detectable by conspecifics at distances within a few centimeters (Fleming et al. 2013), and these close signals could be detected using a variety of receptors (Yack 2004, 2016). However, no sound receptors have been located for bark beetles; here, we propose potential locations.
Bark beetle acoustic communication remains one of the least studied and underappreciated forms of communication in this group of insects. Despite the ubiquity and purported importance of acoustic signals in bark beetles (Rudinsky 1969; Rudinsky and Michael 1972; Ryker 1984), little is known about their physical properties and how these properties vary among behaviors and under different tree substrate conditions. Furthermore, nothing is known about possible sound or vibration receptors. Here we summarize the current knowledge of acoustic communication in bark beetles and hope to advance our understanding of the potential role of vibrations in bark beetle systems.
2 Sound Production and Structures
Air- and substrate-borne vibrations produced via stridulation are known to occur in most bark beetle genera, and vibrational signals are proposed to play important roles in their life history (Barr 1969; Ryker 1988; Lyal and King 1996; Kerchev 2015). Like most coleopterans, sounds are delivered via stridulation, which involves the use of two structures, the ‘plectrum’ or the ‘scraper’ that is moved across a ridged surface commonly known as the ‘file’ or ‘pars stridens’ (Wessel 2006; Rosado-Neto and dos Santos 2010). Additionally, it is possible that some beetle species rub or scrape their exoskeleton against substrates to evoke vibrations (Drosopoulos and Claridge 2006). Within the bark beetle subfamily Scolytinae, there are five known stridulatory mechanisms. These include the (1) gular-prosternal, where the pars striden is located ventrally on the head and is scraped against the plectrum, consisting of a single or multi-ridged structure at the anterior end of the prosternum; (2) elytral-abdominal (Fig. 21.2), where the pars stridens located on the ventral side of the elytra (usually more prominent on one elytron) is scraped by the plectrum (sclerotized peg) located on the seventh segment of the abdominal tergite. A slightly different elytral-abdominal mechanism is proposed for some female Dendroctonus beetles in that an elytral file, similar to that found in males, is located on the sutural margin of the right elytra and a sternal plectrum is located on the inside wall of the last sternite (Rudinsky and Michael 1973); (3) vertex-pronotal (Fig.21.3), where the pars striden, an elongate, ridged structure present on the vertex of the head, is scraped against the plectrum, a multi-ridged structure present at the ventral anterior side of the pronotum (Barr 1969); (4) abdominal, where a sclerotized peg present on the eighth abdominal segment is scraped against a pars stridens on the posterior region of the last sternite (Rudinsky and Michael 1973); and (5) elytral-tibial, where the pars stridens on the outer margin of the elytra, starting at the level of the boundary between abdominal sternites III and IV, is rubbed by the plectrum, which is a large acicular spine on the inner surface of the hind tibiae (Sasakawa and Yoshiyasu 1983; Kerchev 2015). Each of these mechanisms produces somewhat distinct acoustic patterns and frequency components. One factor that can affect acoustic frequency is body size, but only if the mechanism relates proportionally to body size, or if body cavity size directly influences frequency (Yturralde 2013; Lindeman 2016). Studies have shown that beetles using the elytral-abdominal stridulation method (e.g., Dendroctonus beetles) have an inverse correlation between body size and frequency, but other stridulatory methods such as the vertex-pronotal stridulation method (e.g., Ips beetles) are not correlated with frequency.
The extent to which beetles stridulate and the physical mechanism employed often differs between the sexes within a species, depending on which sex initiates the construction of the nuptial gallery (Barr 1969). For instance, the vertex-pronotal mechanism (Fig. 21.3) is generally found in non-host selecting females of polygamous species in the genus Ips and Gnathotrichus (Wilkinson 1962; Wilkinson et al. 1967; Barr 1969; Swaby and Rudinsky 1976). The elytral-abdominal type of stridulatory mechanism (Fig. 21.2) is observed in non-host selecting males of monogamous species in the genus Dendroctonus, Hylesinus and Polygraphus (Vernoff and Rudinsky 1980; Ryker 1988; Kerchev 2015). While the gular-prosternal mechanism can be observed in both sexes of some monogamous (e.g., Scolytus spp.) and polygamous (e.g., some Ips spp.) species (Barr 1969). In some species, one of the sexes may have two stridulatory mechanisms, such as Dryocoetes autographus females, which have the elytral-abdominal and vertex-pronotal stridulatory mechanism (Sasakawa and Yoshiyasu 1983), or Cryphalus fulvus males, which use the elytral-abdominal mechanism during aggression with other males, but use the vertex-pronotal mechanism during male-female interactions (Sasakawa and Sasakawa 1981).
The mechanics of sound production have only been described for a few bark beetle species. Lindeman (2016) determined that chirp production (both simple and interrupted) in Dendroctonus beetles was produced by elastic potential energy, like a stretched spring. Potential energy is stored as the plectrum locks onto the file and remains stationary while the abdomen moves posteriorly, resulting in an increased angle between the plectrum and abdomen (Lindeman 2016). Thus for the elytral-abdominal stridulation mechanism, sound is produced during the down stroke of the plectrum against the pars stridens. Sound that occurs on the upstroke is termed ‘trailing chirps’, which are infrequent but may occur (Lindeman 2016). For intermediate calls, the intervals between phrases occur from a stop in movement in the down stroke of the plectrum against the elytra. This was further confirmed by Lindeman (2016), who determined that the number of teeth on the pars stridens match closely the number of pulses (strikes). A similar elastic mechanism works for species that use the gular-prosternal or vertex-pronotal stridulation mechanisms. As the head prepares to move backward or forward, potential energy is stored as the plectrum begins to push against the pars stridens.
3 Sound Perception by Bark Beetles
Given that beetles stridulate and produce a variety of purported call types (see Sect. 21.4), it can be hypothesized that beetles have receptor organs (see Figs. 21.4, 21.5, 21.6). However, no receptor organs have been located in bark beetles. Surprisingly, receptor organs have only been described in a few Coleoptera species. For example, a Johnston’s organ has been described in whirligig beetles (Gyrinidae) (Kolmes 1983; Bendele 1986) and tympanal hearing organs in tiger beetles (Cicindelidae) and scarab beetles (Dynastinae) (Yager and Spangler 1995). In beetles with tympana, the hearing organs act mainly as sense organs for recognizing the ultrasonic sounds from predatory bats (Forrest et al. 1997). Like many other species living within wood and trees (e.g., termites, social wasps, bees, and ants), or living on surfaces similar to the phloem (plant stems; e.g., leaf hoppers), bark beetles likely possess sensitive vibration detectors. More insects use the vibratory channel than use airborne sound for communication (Michelsen et al. 1982; Virant-Doberlet and Čokl 2004; Yack 2016).
Bark beetle stridulation sounds are broadband, exceeding 85 kHz in some recordings (Yturralde 2013; Fleming et al. 2013). The high sonic and ultrasonic frequencies of bark beetle signals provoke questions about a hearing mechanism capable of perceiving these signals. However, the range at which beetles hear is not known. Here we present results of a playback study, where male D. adjunctus beetles were placed in phloem sandwiches (Aflitto and Hofstetter 2014) and subjected to artificially generated calls of different frequencies and call durations. Playback calls ranged in band frequencies of 1–100 Hz, 100–5000 Hz, 5000–10,000 Hz, 10,000–25,000 Hz, and 25,000–40,000 Hz and durations of 1, 10, 40, 160, and 640 ms. We found that beetles would call back only to sounds in the band frequencies of 100–5000, 5000–10,000, and 10,000–25,000 Hz and durations of 10, 40, or 160 ms. Although this is not a perfect test of what beetles can hear, it does give some insight into the call characteristics that beetles may be responsive to or are capable of hearing. There is a clear need for more advanced research into the functions, characteristics and receptor mechanisms of the acoustic communication in bark beetles, and beetles in general.
4 Acoustic Ecology and Use by Bark Beetles
Given that bark beetles communicate in small galleries within trees, beetles might be hypothesized to communicate by near-field airborne sounds and solid-borne vibrations. Bark beetle stridulations typically produce sounds that are broadband, with multiple peak frequencies ranging from 1 to 80 kHz (Fleming et al. 2013; Lindeman and Yack 2015). Many species are reported to signal in more than one behavioral context, and sounds are often assumed to be used during species recognition, premating interactions, pair formation, mate selection, intraspecies aggression, territoriality and predator deterrence (Barr 1969; Rudinsky 1969; Rudinsky and Michael 1972; Rudinsky et al. 1973; Rudinsky and Ryker 1976; Ryker and Rudinsky 1976a, b; Yandell 1984; Ryker 1988; Lindeman and Yack 2015). Bark beetles typically produce three call forms: single clicks, simple chirps or interrupted chirps. Single clicks are usually produced by the sex that has less pronounced stridulatory structures (e.g., female Dendroctonus or male Ips) and the stridulation mechanism is poorly understood. Clicks usually occur as a single pulse, but multiple consecutive pulses can be produced. Unlike clicks, chirps contain many pulses (i.e., tooth strikes). Both interrupted and simple (uninterrupted) chirp forms (Fig. 21.7) may occur within wave trains; however, in some genera, interrupted chirps may be rare (as in Ips pini; Dobai et al. 2018).
Simple chirps are significantly shorter than interrupted chirps (Fig. 21.7), with chirps containing one set of tooth strikes and interrupted chirps containing two to five sets of strikes within a phrase (e.g., Michael and Rudinsky 1972). Lindeman and Yack (2015) quantitatively defined chirp type produced based on the minimum inter-tooth strike interval that could be considered a “gap” to classify chirps as interrupted. They found that simple chirps contained inter-tooth strike intervals less than 5 ms, while the interrupted chirps also comprised inter-tooth strike intervals less than 5 ms, but more than one quarter (~28%) were greater than 5 ms. Both chirp types can have a similar number of tooth strikes, although interrupted chirps tend to include more strikes (Fleming et al. 2013). The interrupted chirps typically have significantly lower mean tooth strike rates and higher mean inter-tooth strike intervals (Fleming et al. 2013). Within a beetle species, simple chirps usually have lower dominant peak frequencies than interrupted chirps (Yturralde and Hofstetter 2015; Fleming et al. 2013).
Whether simple or interrupted chirps vary between contexts is poorly understood for most species (Fleming et al. 2013; Lindeman and Yack 2015; Yturralde and Hofstetter 2015). Past studies refer to the chirp types as “attraction,” “premating,” “rivalry,” or “distress” chirps (Barr 1969; Ryker and Rudinsky 1976b; Oester et al. 1978; Ryker 1988; Michael and Rudinsky 1972); however, chirp characteristics may differ between contexts and require verification for many species. Understanding how signals vary between contexts can be further complicated in the literature, as signals were recorded under artificial settings that may not represent natural conditions (Wilkinson et al. 1967; Swaby and Rudinsky 1976; Yturralde and Hofstetter 2015). Additionally, beetles appear to produce a variety of calls during encounters or under different situations. Several authors have found that beetle individuals produced both simple and interrupted chirps, but vary in the ratio of interrupted to simple chirps under a variety of situations (Fleming et al. 2013; Lindeman and Yack 2015; Yturralde and Hofstetter 2015). Beetle condition or body size may also influence the ratios of simple and interrupted chirps produced or the characteristics of the chirps (e.g., larger male Dendroctonus valens tended to produce interrupted chirps with more components; Lindeman and Yack 2015).
Acoustic signals appear to mediate pheromone production in many of the aggressive tree-killing bark beetles (Rudinsky and Michael 1972; Pitman and Vité 1974; Ryker and Rudinsky 1976a, b; Pureswaran et al. 2016; Liu et al. 2017). Acoustic communication indirectly functions in moderating aggregation by inducing the release of anti-aggregation or masking pheromones in Dendroctonus species (Rudinsky 1969). Males stridulate at the entry hole and induce females to release anti-aggregation pheromones. This consequently results in the loss of attraction by males to the gallery (Rudinsky 1969). Males stridulate in response to pheromones released by virgin females, and females respond by altering (i.e., reducing attractiveness) their pheromone composition (Liu et al. 2017).
It is unclear whether acoustic signals are used to distinguish sympatric bark beetle species. Several studies indicate that acoustic signals may not contain enough information on species identity. In a laboratory cross-mating study, Lewis and Cane (1990) found that the acoustic signals of four closely related Ips species, although unique in their characteristics, were not effective in preventing forced heterospecific pairings. This suggests that acoustic signals may not serve in species recognition. Alternatively, Pureswaran et al. (2016) found evidence that sympatric Dendroctonus species produce acoustic chirps that differ and could potentially be used for mate location and differentiation by males. For example, chirps of D. brevicomis females (Fig. 21.8) are longer, more frequent and contained more tooth strikes than those of D. frontalis (Fig. 21.8). They also found that males could distinguish between conspecific and heterospecific females when given the choice of pairs of females within tree material. Acoustic call characteristics could be used by males to decide whether or not to enter a gallery. Once inside the female’s gallery, a male may use information from female acoustic signals (or other male signals) to decide whether to remain in the gallery. Differences in female chirp parameters may therefore be reinforced by selection (Pureswaran et al. 2016).
Chirp characteristics and type (e.g., simple or interrupted chirps) appear to play a role in mate acceptance. For instance, male D. valens that produce interrupted chirps have greater success of entry into female galleries (Lindeman and Yack 2015). In each of the trials where males performed exclusively simple chirps, including those where the male was eventually accepted, females actively resisted entry of the male (Lindeman and Yack 2015). Sivalinghem (2011) found that only Ips females that produced acoustic signals were admitted into the gallery (by the male). With Hylastes spp., the number of interrupted chirps increases, while the simple chirps decline prior to mating (Sapkota 2017). Lindeman and Yack (2015) suggest that for Dendroctonus, a male’s acoustic performance may provide the female with information on his vigor, and that gaps in interrupted chirps are produced by a more complex motor performance than required for simple chirps. This more challenging action may be a reliable indicator of fitness (Byers et al. 2010). In any case, premating signals likely provide information about the physical attributes of the signaler, whether it be female (e.g., Ips) or male (e.g., Dendroctonus) (Byers et al. 2010; Lindeman and Yack 2015).
Female signals may function to mediate spacing and territoriality between conspecifics. In most bark beetle species, galleries rarely collide with each other, and in some cases, galleries nearing collision show a sudden change in direction (Rudinsky and Michael 1973; Byers 1989, 2007; Davis and Hofstetter 2009). It is possible that tunneling beetles produce vibrations, either from stridulation or friction via chewing transmitted through phloem substrate, that announce their presence to neighboring beetles. Such sound vibrations may only be transmitted within 1 cm of the gallery but this is an effective distance to deter traversing and touching of galleries. Additionally, larvae could use chewing sounds to space themselves while tunneling, thus reducing potential competition or harm to each other. Rudinsky and Michael (1973) showed that the distance of nearby conspecific adult females affects female stridulation patterns. Dendroctonus brevicomis females emitted significantly more chirps when (two) conspecific females were within 10 cm compared to the number of chirps emitted by solitary females. Dendroctonus pseudotsugae females were slightly less sensitive to nearby conspecific females (Rudinsky 1969). Interestingly, neither species changed their vibrational signals when in the presence of heterospecific females at distances of 1–5 cm.
Male-male aggression is common in some bark beetle genera, and males will often produce intense stridulatory vibrations in the presence of other males within their gallery (Rudinsky and Michael 1974; Pureswaran et al. 2016). Studies of Dendroctonus frontalis show that males emit a chain of chirps (with a combination of simple and interrupted types) when placed with other males or in response to males entering an already male-occupied gallery. These chirps (sometimes called rivalry chirps) are accompanied by aggressive behavior by both males. A resident male will respond to an intruding male with intense chirping, biting and pushing. When males are confined together in a gallery, long chirp trains occur and intense pushing and biting ensues (Pureswaran et al. 2016). Once out of the gallery, the resident male may continue to chirp and push the other male. Similar behavior has been observed for many Dendroctonus species (Rudinsky and Michael 1974) and also between sympatric species of Dendroctonus (Pureswaran et al. 2016).
It is unclear whether natural enemies use bark beetle stridulations to locate their prey. Woodpeckers are common predators of many bark beetle species (Schenk and Benjamin 1969; Wegensteiner et al. 2015), and Ramp (1965) showed that the auditory range of the hairy woodpecker had an upper limit reaching 18.5 kHz, which overlaps the frequency of many bark beetle signals (Fig. 21.8). However, attraction of predators to specific beetle stridulations has not been tested. Lewis and Cane (1990) and Sivalinghem (2011) tested whether stridulation by bark beetles would deter or reduce predation events. Lewis and Cane (1990) showed that signaling Ips calligraphus females were dropped significantly more often than non-signaling males by a natural beetle predator, Thanasimus dubius. Alternatively, Sivalinghem (2011) demonstrated that acoustic signals during predation events were not effective in causing predators to release prey, and the results do not support the “startling function” hypothesis proposed by Lewis and Cane (1990). It is possible that the difference between the studies was due to the differences in beetle size (Lewis and Cane 1990 studied a larger Ips beetles), the relatively low sample size in the studies, or that the data may not have been independent as each predator was used more than once in the Lewis and Cane (1990) study. Frazier et al. (1981) investigating predator-prey interactions between Dendroctonus frontalis and T. dubius found that handling time was significantly higher for male D. frontalis than for females. Since male D. frontalis produce chirps, sound production may have been a key variable contributing to the longer handling time of males versus females.
Emerging bark beetle progeny or over-wintering adult beetles may use stridulation or other substrate vibrations to coordinate emergence from host trees. No formal studies have looked at the cues or behaviors associated with beetle emergence on trees. Recordings (R.W. Hofstetter, pers. comm.) in the field of overwintering Ips and emerging D. valens progeny show that beetles may stridulate within the bark prior to emergence.
Mattson and Haack (1987) hypothesized that bark beetles may locate weakened or drought-stressed trees by exploiting the ultrasonic acoustic emissions released by cavitation (Haack et al. 1988). Several studies have shown that when trees experience long periods of dehydration, the water columns in the xylem tissues cavitate, releasing ultrasonic sounds with dominant frequencies ranging from 60 to 300 kHz (Tyree and Dixon 1983; Pena and Grace 1986; Tyree and Sperry 1989). Mattson and Haack (1987) hypothesized that bark- and wood-boring insects could exploit these acoustic cues during host-plant localization. The ultrasonic frequencies of bark beetle signals (Fig. 21.8) implies that bark beetles may be capable of hearing these frequencies, which indirectly supports Mattson and Haack’s (1987) hypothesis. Kaiser (2014) tested whether beetles preferred host material that emitted (synthetic) cavitation sounds. He found a greater number of pioneering female D. ponderosae chose host material that had high acoustic emissions (produced by ultrasound tactile speakers) versus host material that did not emit ultrasonic sounds. The ability of beetles to identify water-stressed hosts via acoustic cues may be critical to their success at low population densities.
5 Vibrations and Movement Within Tree Materials
Many insects use solid-borne vibrations to communicate (Markl 1983; Cocroft et al. 2014; Yack 2016). Bark beetles are capable of producing solid-borne vibrations, but few studies address this specific mode of communication (Fleming et al. 2013; Lindeman 2016). Beetles likely produce two types of vibrations, passive cues produced during chewing of wood or movement within tunnels and active signals produced via stridulation, which were previously referred to as chirps. Vibrations from non-signaling behavior are likely received by predators such as woodpeckers and predatory beetles, as well as neighboring bark beetles and competitors. Sound produced by stridulation, or through friction with the substrate (e.g., mandible-substrate stridulation), by bark beetles conveys messages and is typically stereotyped and conspicuous (Fleming et al. 2013). In most situations with vibrations in wood, the type of vibrations used for communication is almost exclusively restricted to Rayleigh waves (combined longitudinal and transverse waves) or bending waves (Hill 2008). Identifying the type of vibration would provide information on how a given signal would be transmitted (Hill 2008).
We know little about the transmission properties of the phloem in living, dying or dead trees. The phloem’s transmission properties are influenced by wood density, deterioration by bacterial, fungal or other boring agents, and moisture content. Phloem and xylem characteristics affect frequencies differently through filtering or by attenuation (Hebets et al. 2008). Bark beetle acoustic behavior and signal properties are likely adapted to accommodate the sound properties of phloem. For example, the broad-band spectra of bark beetle calls may restrict communication to a close range (e.g., Čokl et al. 2004). Close range communication could be an advantage to reduce eavesdropping by predators, limit unintended signals from conspecifics in non-neighboring galleries, and provide a convenient mechanism for limiting overlapping galleries.
Fleming et al. (2013) observed that higher frequency signals (e.g., interrupted chirps during male-male interactions) could be detected by a laser vibrometer several centimeters away from the beetle, but lower amplitude signals were not detected by a laser at distances of 1 cm. Of those chirps that were detected at 1 cm, the vibrations were low amplitude, with a velocity of ~2.7 mm/s. Fleming et al. (2013) notes that the poor signal quality of substrate-borne vibrations compared with airborne sounds could be due to attenuation through the wood but could also be attributable to recording method (i.e., using a laser). Studies (Fleming et al. 2013; Lindeman 2016) show that both air- and substrate-borne vibrations are available to conspecifics at the distances they would normally be interacting, and these close signals could be detected using tympanal ears, near-field sound detectors (e.g., Johnston’s organs), or vibration receptors (e.g., subgenual organs) (reviewed in Yack 2004).
We conducted an experiment to evaluate the attenuation effects of the phloem tissue on bark beetle calls (Fig. 21.9). The experiment consisted of recording the sounds produced by individual Hylastes ater inside a freshly cut Pinus radiata log. To achieve this, an accelerometer was located on the phloem layer of the log at nonlinearly spaced distances from the sound source. Figure 21.9 shows the averaged power spectral densities and energies obtained from five H. ater individuals. With distance, the energy decays nonlinearly and the bandwidth shrinks toward the spectral centroid. The phloem tissue acts as a low-pass filter, thus, audible and ultrasonic components above 15 kHz are heavily attenuated regardless of the distance.
6 Acoustic Technology and Potential Applied Applications
With the exception of olfaction (Borden 1989), the sensory system of bark beetles, along with many other insects, has yet to be leveraged as a management tool. Since bark beetles are more reliant on air- and substrate-borne sounds inside of trees, a place where other sensory modalities are restricted, the application of acoustic-based treatments has the potential to modify behavior in this insulated environment. While the use of vibrations has been largely limited to the detection of wood-infesting insects (Mankin and Moore 2010; Mankin et al. 2011), a few studies have illustrated the efficacy of acoustic treatments that alter behavior.
In one of these studies, Hofstetter et al. (2013) tested the effects of acoustic signals on the tunneling and oviposition of D. frontalis. Two sound treatments were administered to a phloem sandwich assay (see Aflitto and Hofstetter 2014): modified Dendroctonus beetle calls and an FM radio station. Daily tunneling length was the same in the control (no sound) and radio treatments, averaging 2.1 cm per day. This is contrasted by the modified beetle treatment, which reduced daily tunneling to 0.4 cm per day. The beetle sound treatment also had a strong negative effect on oviposition and only one egg was laid in 15 replicates over seven days compared to 204 eggs from 13 control replicates and 117 eggs from ten radio treatment replicates. Reducing the amount beetles tunnel and oviposit is an important part of the life cycle to target since tunneling contributes to the failure of the tree’s vascular system (Bridges et al. 1985; Franceschi et al. 2005).
Automatic acoustic detection and identification of bark beetles is another relevant and understudied area with potential applications (Mankin and Moore 2010; Mankin et al. 2011). Acoustic approaches could be used to study bark beetles without disrupting their natural environment, or to detect them in cases where conventional detection procedures (e.g., visual inspection) are impractical. Additionally, acoustic features could be used as a rapid method of species identification. The wide variety of shapes, sizes, and stridulatory organs makes the call of each bark beetle species unique (Fig. 21.8), and ultimately, a potential taxonomic character. Nonetheless, there is very limited research activity in automatic acoustic bark beetle detection and identification (Lindeman 2016), and no works have addressed the use of these types of methods in the previously mentioned contexts.
Targeting host selection behavior is another aspect of the bark beetle life history where acoustic treatments may be applied. Aflitto et al. (2014) tested whether beetle entry into logs could be affected by acoustic treatments. A choice test assay was used to observe the response of three bark beetle species to four sound treatments: conspecific stress and attraction calls (interrupted calls), longhorn beetle stress call and abiotic tones. The sound treatments affected bark beetle species differently, but the greatest response was found with D. frontalis to their stress call, where entry into logs was reduced by 72%. An interesting part of Dendroctonus beetle host selection behavior is the use of multimodal communication. For example, D. pseudotsugae females colonize a host and quickly begin releasing aggregation pheromones to attract males. Once she identifies a mate via his attraction call, she begins releasing anti-aggregation pheromones to reduce competition for her offspring (Rudinsky et al. 1976). There is potential to hasten the release of these repellent semiochemicals by sonically treating trees with the attraction sounds of male beetles. More research is needed to understand the mechanisms behind how beetles receive acoustic cues and how played-back sounds travel through tree material before it can be used.
There are several challenges to overcome before acoustic treatments are deployed. The current research has tested treatment effects only on portions of trees (e.g., logs, phloem sandwiches). Applying the technology to entire trees will require more testing and inevitably additional modifications. Further, the application of acoustic treatments for stand or landscape-scale outbreaks pose additional challenges such as an efficient way to administer treatments and powering audio devices.
The variety of acoustic tests that have been performed, both under controlled laboratory conditions and in the field, have strongly suggested the efficacy of using sound to affect bark beetle (and other invertebrate) behavior. This research has led to successfully securing patent on a combination of acoustic technologies and associated protocols to disrupt and deter wood-infesting insects in trees and wood products (Hofstetter et al. 2010, Patent No.: US 9480, 248 B2). As an applied technology, the device and protocols are flexible enough to encompass a wide range of insects and other invertebrates but has so far been primarily applied to research upon members of the Scolytinae and Platypodinae tribes (Coleoptera: Curculionidae).
The patent describes the use of biologically relevant sounds derived from the normal signals produced by the insects themselves, modified biologically relevant sounds, synthetically produced complex sounds, and various combinations of all of these acoustic sources, played back into the acoustic substrates occupied by the target organisms (Hofstetter et al. 2013). The working hypothesis is that exposure to the biologically relevant sounds solicits perceptual attention and “neural” readiness that is forced to constantly shift in an unpredictable and exhausting manner since the sounds are presented as a random and quickly shifting playback. Responses to the playback of biologically relevant sounds can be both predictable and dramatic.
Synthetic sounds are generated from a novel network of electronic circuits based upon the mathematics of deterministic chaos (Hofstetter et al. 2013). These circuits produce constantly changing and non-repetitive complex sounds that have very large frequency and amplitude dynamics resulting from a hyper-chaotic state that is autonomous in its behavior. Once set in motion, the circuit network continues to produce novel sounds that appear to be highly disruptive to the target insects since they pass through so many constantly changing auditory behaviors (Hofstetter et al. 2010). Some of these sounds are reminiscent of the insects’ own sounds, those of competitive species and potential predatory threats, others that are simply outside of their experiential domain but highly intrusive, and others that appear to mask the normal acoustic communication of the insects themselves. The most important feature of these circuits and their sounds is that they make habituation to the simultaneous presentation of all of the treatment sounds unlikely.
Further development of the patent toward a commercial prototype is ongoing. This research has been directed toward several areas of potential application. These objectives include the effect of sound at reducing bark beetle attacks and colonization on healthy trees, the effects of these sounds on bark beetle behavior within tree tissues, and the potential use of acoustic methods to reduce beetle reproduction and overall populations. Current work is being dedicated to coding both the playback of biologically relevant sounds and the chaotic circuit network into digital form appropriate for implementation as inexpensive and small digital devices that can be used for the protection and treatment of high-value individual trees, orchards and other agricultural contexts, and wood products. Deployment at the scale of large forests remains impractical at this time.
References
Aflitto NC, Hofstetter RW (2014) Use of acoustics to deter bark beetles from entering tree material. Pest Manag Sci 70(12):1808–1814
Aflitto NC, Hofstetter RW, McGuire R, Dunn DD, Potter KA (2014) Technique for studying arthropod and microbial communities within tree tissues. J Vis Exp 93:e50793
Barr BA (1969) Sound production in Scolytindae (Coleoptera) with emphasis on the genus Ips. Can Entomol 101(6):636–672
Bendele H (1986) Mechanosensory cues control chasing behaviour of whirligig beetles (Coleoptera, Gyrinidae). J Comp Physiol A 158(3):405–411
Bentz BJ, Regniere J, Fettig CJ, Hansen EM, Hayes JL, Hicke JA, Kelsey RG, Negron JF, Seybold SJ (2010) Climate change and bark beetles of the western United States and Canada: direct and indirect effects. Bioscience 60(8):602–613
Birch MC (1984) Aggregation in bark beetles. In: Bell WJ, Cardé RT (eds) Chemical ecology of insects. Springer, Boston, MA, pp 331–353
Borden JH (1989) Semiochemicals and bark beetle populations: exploitation of natural phenomena by pest management strategists. Ecography 12(4):501–510
Bridges JR, Nettleton WA, Connor MD (1985) Southern pine beetle (Coleoptera: Scolytidae) infestations without the bluestain fungus, Ceratocystis minor. J Econ Entomol 78(2):325–327
Byers JA (1989) Chemical ecology of bark beetles. Cell Mol Life Sci 45:271–283
Byers JA (2007) Simulation of mating disruption and mass trapping with competitive attraction and camouflage. Environ Entomol 36(6):1328–1338
Byers J, Hebets E, Podos J (2010) Female mate choice based upon male motor performance. Anim Behav 79(4):771–778
Cocroft RB, Gogala M, Hill PSM, Wessel A (eds) (2014) Studying vibrational communication. Springer, Berlin
Čokl A, Prešern J, Virant-Doberlet M, Bagwell GJ, Millar JG (2004) Vibratory signals of the harlequin bug and their transmission through plants. Physiol Entomol 29:372–380
Davis TS, Hofstetter RW (2009) Effects of gallery density and species ration on the fitness of fecundity of two sympatric bark beetles (Coleoptera: Curculionidae). Environ Entomol 38(3):639–650
Dobai A, Sivalinghem S, Guedes RN, Yack JE (2018) Acoustic communication in the pine engraver bark beetle: do signals vary between behavioural contexts? Physiol Entomol 43:30–41
Drosopoulos S, Claridge MF (2006) Insect sounds and communication: physiology, behaviour, ecology and evolution. CRC Press LLC, Boca Raton, FL
Eidmann HH (1992) Impact of bark beetles on forests and forestry in Sweden. J Appl Entomol 114(1-5):193–200
Fleming AJ, Lindeman AA, Carroll AL, Yack JE (2013) Acoustics of the mountain pine beetle (Dendroctonus ponderosae) (Curculionidae, Scolytinae): sonic, ultrasonic, and vibration characteristics. Can J Zool 91(4):235–244
Forrest T, Read M, Farris H, Hoy R (1997) A tympanal hearing organ in Scarab beetles. J Exp Biol 200(3):601–606
Franceschi VR, Krokene P, Christiansen E, Krekling T (2005) Anatomical and chemical defenses of conifer bark against bark beetles and other pests. New Phytol 167(2):353–376
Frazier JL, Nebeker TE, Mizell RF, Calvert WH (1981) Predatory behavior of the clerid beetle Thanasimus dubius (Coleoptera: Cleridae) on the southern pine beetle (Coleoptera: Scolytidae). Can Entomol 113:35–43
Haack RA, Blank RW, Fink FT, Mattson WJ (1988) Ultrasonic acoustical emissions from sapwood of eastern white pine, northern red oak, red maple, and paper birch: implications for bark-and wood-feeding insects. Fla Entomol 71(4):427–440
Hebets EA, Elias DO, Mason AC, Miller GL, Stratton GE (2008) Substrate dependent signalling success in the wolf spider, Schizocosa retrorsa. Anim Behav 75:605–615
Hill PSM (2008) Vibrational communication in animals. Harvard University Press, Cambridge
Hofstetter RW, McGuire R, Dunn DD (2010) Use of acoustics to disrupt and deter wood-infesting insects and other invertebrates from and within trees and wood products. US Patent 2011/63838 (2010); WIPO Patent Application WO/2012/078814 (2012)
Hofstetter RW, Dunn DD, McGuire R, Potter KA (2013) Using acoustic technology to reduce bark beetle reproduction. Pest Manag Sci 70(1):24–27
Hulcr J, Atkinson TH, Cognato AI, Jordal BH, McKenna DD (2015) Morphology, taxonomy, and phylogenetics of bark beetles. In: Vega FE, Hofstetter RW (eds) Bark beetles: biology and ecology of native and invasive species. Academic Press, San Diego, pp 41–84
Kaiser M (2014) Acoustics of Mountain Pine Beetle and Lodgepole Pine. PhD thesis, University of Calgary, Graduate Program in Biological Sciences, Calgary, Alberta, Canada
Kerchev IA (2015) Description of the stridulatory apparatus of the Far Eastern bark beetles Polygraphus proximus Blandford, 1894 and P. jezoensis Niisima, 1909 (Coleoptera, Curculionidae: Scolytinae). Entomol Rev 95(9):1191–1196
Kirkendall LR (1983) The evolution of mating systems in bark and ambrosia beetles (Coleoptera: Scolytidae and Platypodidae). Zool J Linn Soc Lond 77:293–352
Kolmes SA (1983) Ecological and sensory aspects of prey capture by the whirligig beetle Dineutes discolor (Coleoptera: Gyrinidae). J NY Entomol Soc 91(4):405–412
Lewis EE, Cane JH (1990) Stridulation as a primary antipredator defence of a beetle. Anim Behav 40:1003–1004
Lindeman AA (2016) Acoustic signalling in the destructive bark beetle genus Dendroctonus (Curculionidae: Scolytinae) with emphasis on Dendroctonus valens. PhD thesis, Carleton University, Department of Biology, Ottawa, Canada
Lindeman AA, Yack JE (2015) What is the password? Female bark beetles (Scolytinae) grant males access to their galleries based on courtship song. Behav Process 115:123–131
Liu Z, Xin Y, Xu B, Raffa KF, Sun J (2017) Sound-triggered production of antiaggregation pheromone limits overcrowding of Dendroctonus valens attacking pine trees. Chem Senses 42(1):59–67
Lyal CHC, King T (1996) Elytro-tergal stridulation in weevils (Insecta: Coleoptera: Curculionoidea). J Nat Hist 30(5):703–773
Mankin RW, Moore A (2010) Acoustic detection of Oryctes rhinoceros (Coleoptera: Scarabaeidae: Dynastinae) and Nasutitermes luzonicus (Isoptera: Termitidae) in palm trees in urban Guam. J Econ Entomol 103:1135–1143
Mankin RW, Hagstrum DW, Smith MT, Roda AL, Kairo MTK (2011) Perspective and promise: a century of insect acoustic detection and monitoring. Am Entomol 57:30–44
Markl H (1983) Vibrational communication. In: Huber F, Markl H (eds) Neuroethology and behavioral physiology. Springer, Berlin, pp 332–353
Mattson WJ, Haack RA (1987) The role of drought in outbreaks of plant-eating insects. Bioscience 37(2):110–118
Michael R, Rudinsky J (1972) Sound production in Scolytidae: specificity in male Dendroctonus beetles. J Insect Physiol 18(11):2189–2201
Michelsen A, Fink F, Gogala M, Traue D (1982) Plants as transmission channels for insect vibrational songs. Behav Ecol Sociobiol 11:269–281
Oester PT, Ryker LC, Rudinsky JA (1978) Complex male premating stridulation of the bark beetle Hylurgops rugipennis (Mann.). Coleop Bull 32(2):93–98
Pena J, Grace J (1986) Water relations and ultrasound emissions of Pinus sylvestris L. before, during and after a period of water stress. New Phytol 103(3):515–524
Pitman GB, Vité JP (1974) Biosynthesis of methylcyclohexenone by male Douglas-fir beetle. Environ Entomol 3(5):886–887
Pureswaran DS, Hofstetter RW, Sullivan BT, Potter KA (2016) The role of multimodal signals in species recognition between tree-killing bark beetles in a narrow sympatric zone. Environ Entomol 45(3):582–591
Raffa KF, Phillips TW, Salom SM (1993) Strategies and mechanisms of host colonization by bark beetles. In: Schowalter TD, Filip GM (eds) Beetle-pathogen interactions in conifer forests. Academic Press, London, pp 102–128
Raffa KF, Aukema BH, Bentz BJ, Carroll AL, Hicke JA, Turner MG, Romme WH (2008) Cross-scale drivers of natural disturbances prone to anthropogenic amplification: the dynamics of bark beetle eruptions. Bioscience 58(6):501–517
Raffa KF, Gregoire G-C, Lindgren BS (2015) Natural history and ecology of bark beetles. In: Vega FE, Hofstetter RW (eds) Bark beetles: biology and ecology of native and invasive species. Academic Press, San Diego, pp 1–28
Ramp WK (1965) The auditory range of a Hairy woodpecker. Condor 67(2):183–185
Rosado-Neto GH, Santos GBD (2010) The elytro-tergal stridulatory apparatus of the genus Bondarius Rosado-Neto (Coleoptera, Curculionidae). Rev Bras Entomol 54(2):337–338
Rudinsky JA (1969) Masking of the aggregation pheromone in Dendroctonus pseudotsugae Hopk. Science 166(3907):884–885
Rudinsky JA, Michael RR (1972) Sound production in Scolytidae: chemostimulus of sonic signal by the Douglas-fir beetle. Science 175(4028):1386–1390
Rudinsky JA, Michael RR (1973) Sound production in Scolytidae: stridulation by female Dendroctonus beetles. J Insect Physiol 19(3):689–705
Rudinsky JA, Michael RR (1974) Sound production in Scolytidae: ‘Rivalry’ behaviour of male Dendroctonus beetles. J Insect Physiol 20(7):1219–1230
Rudinsky JA, Ryker LC (1976) Sound production in Scolytidae: rivalry and premating stridulation of male Douglas-fir beetle. J Insect Physiol 22(7):997–999, 1001–1003
Rudinsky JA, Morgan M, Libbey LM, Michael RR (1973) Sound production in Scolytidae: 3-methyl-2-cyclohexen-l-one released by the female Douglas fir beetle in response to male sonic signal. Environ Entomol 2(4):505–510
Rudinsky JA, Ryker LC, Michael RR, Libbey LM, Morgan ME (1976) Sound production in Scolytidae: female sonic stimulus of male pheromone release in two Dendroctonus beetles. J Insect Physiol 22:1675–1681
Rudinsky JA, Oester PT, Ryker LC (1978) Gallery initiation and male stridulation of polygamous Spruce bark beetle Polygraphus rufipennis. Ann Entomol Soc Am 71(3):317–321
Ryker LC (1984) Acoustic and chemical signals in the life cycle of a beetle. Sci Am 250(6):112–123
Ryker LC (1988) Acoustic studies of Dendroctonus bark beetles. Fla Entomol 71(4):447–461
Ryker LC, Rudinsky JA (1976a) Sound production in Scolytidae – acoustic signals of male and female Dendroctonus valens Leconte. J Appl Entomol 80(2):113–118
Ryker LC, Rudinsky JA (1976b) Sound production in Scolytidae – aggressive and mating behaviour of mountain pine beetle. Ann Entomol Soc Am 69(4):677–680
Ryker LC, Libbey LM, Rudinsky JA (1979) Comparison of volatile compounds and stridulation emitted by the Douglas-fir beetle from Idaho and Western Oregon populations. Environ Entomol 8(5):789–798
Sapkota S (2017) Acoustic communication and behaviour of the Golden Haired Pine Bark Beetle, Hylurgus ligniperda (Coleoptera: Curculionidae). MS thesis, Massey University, Department of Zoology, Palmerston North, New Zealand
Sasakawa M, Sasakawa T (1981) Stridulatory organs of the minute pine bark beetle Cryphalus fulvus Coleoptera Scolytidea and role of male sound in aggression behaviour. Kontyû 49(3):461–469
Sasakawa M, Yoshiyasu Y (1983) Stridulatory organs of Japanese pine bark beetles Coleoptera Scolytidae. Kontyû 51(4):493–501
Schenk JA, Benjamin DM (1969) Notes on the biology of Ips pini in central Wisconsin jack pine forests. Ann Entomol Soc Am 62(3):480–485
Sivalinghem S (2011) Acoustic communication in the Pine Engraver Bark Beetle, Ips pini (Coleoptera: Scolytinae). MS thesis, Carleton University, Department of Biology, Ottawa, Canada
Swaby JA, Rudinsky JA (1976) Acoustic and olfactory behaviour of Ips pini (say) (Coleoptera-Scolytidae) during host invasion and colonization. J Appl Entomol 81(4):421–432
Tyree MT, Dixon MA (1983) Cavitation events in Thuja occidentalis L.? Plant Physiol 72(4):1094–1099
Tyree MT, Sperry JS (1989) Vulnerability of xylem to cavitation and embolism. Annu Rev Plant Biol 40(1):19–36
Vega FE, Hofstetter RW (2015) Bark beetles: biology and ecology of native and invasive species. Academic Press, San Diego, CA
Vernoff S, Rudinsky JA (1980) Sound production and pairing behaviour of Leperinus-californicus swaine and Leperisinus oregonus blackman (Coleoptera, Scolytidae) attacking oregon ash. J Appl Entomol 90(1):58–74
Virant-Doberlet M, Čokl A (2004) Vibrational communication in insects. Neotrop Entomol 33:121–134
Wegensteiner R, Wermelinger B, Herrmann M (2015) Natural enemies of bark beetles: predators, parasitoids, pathogens, and nematodes. In: Vega FE, Hofstetter RW (eds) Bark beetles: biology and ecology of native and invasive species. Academic Press, San Diego, CA, pp 247–304
Wermelinger B (2004) Ecology and management of the spruce bark beetle Ips typographus – a review of recent research. Forest Ecol Manag 202(1):67–82
Wessel A (2006) Stridulation in the Coleoptera – an overview. In: Drosopoulos S, Claridge MF (eds) Insect sound and communication. CRC Press, Boca Raton, FL, pp 397–404
Wilkinson R (1962) Stridulating organs in three southeastern Ips bark beetles. Fla Entomol 45(1):43–44
Wilkinson RC, McClelland WT, Murillo R, Ostmark EO (1967) Stridulation and behavior in two southeastern Ips bark beetles (Coleoptera: Scolytidae). Fla Entomol 50(3):185–195
Wood SL (1982) The bark and ambrosia beetles of North and Central America (Coleoptera: Scolytidae), a taxonomic monograph. In: The Bark and Ambrosia Beetles of North and Central America (Coleoptera: Scolytidae), a taxonomic monograph. Great Basin Nat Mem 6:1–1356
Yack JE (2004) The structure and function of auditory chordotonal organs in insects. Microsc Res Tech 63:315–337
Yack J (2016) Vibrational signaling. In: Pollack GS, Mason AC, Popper AN, Fay RR (eds) Insect hearing. Springer, New York, pp 99–123
Yager DD, Spangler HG (1995) Characterization of auditory afferents in the tiger beetle, Cincindela marutha Dow. J Comp Physiol 176:587–599
Yandell KL (1984) Sound production of Dendroctonusponderosae Hopkins (Coleoptera, Scolytidae) – a comparison of populations from three host pines in Oregon. J Appl Entomol 97(2):180–187
Yturralde KM (2013) The acoustic ecology of bark beetles and bed bugs. PhD thesis, Northern Arizona University, School of Forestry, Flagstaff AZ
Yturralde KM, Hofstetter RW (2015) Characterization of stridulatory structures and sounds of the larger Mexican pine beetle, Dendroctonus approximatus (Coleoptera: Curculionidae: Scolytinae). Fla Entomol 98(2):516–527
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Hofstetter, R.W., Aflitto, N., Bedoya, C.L., Yturralde, K., Dunn, D.D. (2019). Vibrational Behavior in Bark Beetles: Applied Aspects. In: Hill, P., Lakes-Harlan, R., Mazzoni, V., Narins, P., Virant-Doberlet, M., Wessel, A. (eds) Biotremology: Studying Vibrational Behavior . Animal Signals and Communication, vol 6. Springer, Cham. https://doi.org/10.1007/978-3-030-22293-2_21
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