Overview
- Covers biolistic gene transfer into plants, nematodes, or in vitro and in vivo mammalian cells
- Includes lab-ready protocols with clear step-by-step direction from leading researchers in the field
- Features key tips and implementation advice to guarantee successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 940)
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About this book
Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.
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Keywords
Table of contents (29 protocols)
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Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology
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Biolistic DNA Delivery in Nematodes
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Biolistic DNA Delivery in Mammalian Cells In Vitro
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Biolistic DNA Delivery in Mammalian Cells In Vivo
Editors and Affiliations
Bibliographic Information
Book Title: Biolistic DNA Delivery
Book Subtitle: Methods and Protocols
Editors: Stephan Sudowe, Angelika B. Reske-Kunz
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-110-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-109-7Published: 29 October 2012
Softcover ISBN: 978-1-4939-6068-2Published: 23 August 2016
eBook ISBN: 978-1-62703-110-3Published: 28 October 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 415
Topics: Human Genetics, Genetic Engineering