Abstract
Biolistic DNA delivery is an approach to deliver plasmid to culture cells, plants, or animals. Plasmid DNA is usually transferred through bombardment of DNA-coated particles by highly pressurized gas in various kinds of delivery vehicles. The low pressure gene gun can deliver plasmid at lower pressure. Here, we describe methods of biolistic DNA delivery to mice using the low pressure gene gun.
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References
Williams RS et al (1991) Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles. Proc Natl Acad Sci USA 88:2726–2730
Larregina AT et al (2001) Direct transfection and activation of human cutaneous dendritic cells. Gene Ther 8:608–617
Liu CW et al (2007) Stable chloroplast transformation in cabbage (Brassica oleracea L. var. capitata L.) by particle bombardment. Plant Cell Rep 26:1733–1744
Wang BW et al (2007) Mechanical stretch enhances the expression of resistin gene in cultured cardiomyocytes via tumor necrosis factor-alpha. Am J Physiol Heart Circ Physiol 293:2305–2312
Tu CF et al (2007) Autologous neu DNA vaccine can be as effective as xenogenic neu DNA vaccine by altering administration route. Vaccine 25:719–728
Hsieh CY et al (2007) IL-6-encoding tumor antigen generates potent cancer immunotherapy through antigen processing and anti-apoptotic pathways. Mol Ther 15:1890–1897
Kao CY, Huang SH, Lin CM (2008) A low-pressure gene gun for genetic transformation of maize. Plant Biotechnol Rep 2:267–270
Lin CC et al (2008) Delivery of noncarrier naked DNA vaccine into the skin by supersonic flow induces a polarized T helper type 1 immune response to cancer. J Gene Med 10:679–689
Yen MC et al (2009) A novel cancer therapy by skin delivery of indoleamine 2,3-dioxygenase siRNA. Clin Cancer Res 15:641–649
Huang CH et al (2010) Promoting effect of Antrodia camphorata as an immunomodulating adjuvant on the antitumor efficacy of HER-2/neu DNA vaccine. Cancer Immunol Immunother 59:1259–1272
Acknowledgments
This work is supported by Grant NSC99-2323-B006-004 from National Science Council, Taiwan, Republic of China.
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Yen, MC., Lai, MD. (2013). Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun. In: Sudowe, S., Reske-Kunz, A. (eds) Biolistic DNA Delivery. Methods in Molecular Biology, vol 940. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-110-3_14
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DOI: https://doi.org/10.1007/978-1-62703-110-3_14
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-109-7
Online ISBN: 978-1-62703-110-3
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