Abstract
Non-vacuum gene guns such as HandyGun are flexible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation efficiency of plant cells with plasmid DNA using HandyGun will be provided.
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References
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Acknowledgments
The work was supported financially by the Academy of Finland (grants 118766 and 1134759 to J.P.T.V.).
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Vuorinen, A.L., Nieminen, A., Gaba, V., Sikorskaite, S., Valkonen, J.P.T. (2013). Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-vacuum Gene Gun (HandyGun). In: Sudowe, S., Reske-Kunz, A. (eds) Biolistic DNA Delivery. Methods in Molecular Biology, vol 940. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-110-3_4
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DOI: https://doi.org/10.1007/978-1-62703-110-3_4
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-109-7
Online ISBN: 978-1-62703-110-3
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