Abstract
Hepatocyte transplantation has recently become an efficient clinical method in the treatment of patients with metabolic liver diseases. The shortage of donor cells remains an obstacle to treat more patients. Foetal liver tissues may therefore be useful as an alternative source of generating functional hepatocytes after in vitro culture and maturation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Fox, I. J., Chowdhury, J. R, (2004) Hepatocyte transplantation. Am J Transpl 4, 7–13.
Hughes, R. D., Mitry, R. R., Dhawan, A. (2005) Hepatocyte transplantation for metabolic liver disease: UK experience. J Roy Soc Med 98, 341–345.
Horslen, S .P., Fox, I. J. (2004) Hepatocyte transplantation. Transplantation 77, 1481–1486.
Mizuguchi, T., Mitaka, T., Katsuramaki, T., et al. (2005) J Hepatobiliary Pancreat Surg 12, 378–385.
Fausto, N. (2000) Liver regeneration. J Hepatol 32, 19–31.
Bucher, N. L., Robinson, G. S., Farmer, S. R. (1990) Effects of extracellular matrix on hepatocyte growth and gene expression: implications for hepatic regeneration and the repair of liver injury. Semin Liver Dis 10,11–19.
Block, G. D., Locker, J., Bowen, W. C., et al. (1996) Population expansion, clonal growth, and specific differentiation patterns in primary cultures of hepatocytes induced by HGF/SF, EGF and TGF alpha in a chemically defined (HGM) medium. J Cell Biol 132, 1133–1149.
Runge, D., Michalopoulos, G. K., Strom, S. C., et al. (2000) Recent advances in human hepatocyte culture systems. Biochem Biophys Res Commun 274, 1–3.
Reid, L. M., Jefferson, D. M. (1984) Culturing hepatocytes and other differentiated cells. Hepatology 4, 548–559.
Michalopoulos, G. K., DeFrances, M. C. (1997) Liver regeneration. Science 276, 60–66.
Azuma, H., Hirose, T., Fujii, H., etal. (2003) Enrichement of hepatic progenitor cells from adult mouse liver. Hepatology 37, 1385–1394.
Williams, G. M., Weisburger, E. K., Weisburger, J. H. (1971) Isolation and long-term culture of epithelial-like cells from rat liver. Exp Cell Res 69, 106–112.
Grisham, J. W. (1983) Cell types in rat liver: their identification and isolation. Mol Cell Biochem 53, 23–33.
Neupert, G., Langbein, L., Karsten, U. (1987) Characterization of established epithelioid cell lines derived from rat liver: expression of cytokeratin filaments. Exp Pathol 31, 161–167.
Williams, G. M. (1976) Primary and longterm culture of adult rat liver epithelial cells. Method Cell Biol 14, 357–364.
Tsuchiya, A., Heike, T., Fujino, H., et al. (2005) Long-term extensive expansion of mouse hepatic stem/progenitor cells in a novel serum-free culture system. Gastroenterology 128, 2089–2104.
Tsuchiya, A., Heike, T., Baba, S., et al. (2007) Long-term culture of postnatal mouse hepatic stem/progenitor cells and their relative developmental hierarchy. Stem Cells 25, 895–902.
Yovchev, M. I., Grozdanov, P. N., Joseph, B., et al. (2007) Novel hepatic progenitor cell surface markers in the adult rat liver. Hepatology 45, 139–149.
Furukawa, K., Shimada, T., England, P., et al. (1987) Enrichment and characterization of clonogenic epithelial cells from adult rat liver and initiation of epithelial cell strains. In Vitro Cell Dev Biol I 23, 339–348.
Mahieu-Caputo, D., Allain, J. E., Branger, J., et al. (2004) Repopulation of athymic mouse liver by cryopreserved early human fetal hepatoblasts. Hum Gene Ther 15, 1219–1228.
Dan, Y. Y., Riehle, K. J., Lazaro, C., et al. (2006) Isolation of multipotent progenitor cells from human fetal liver capable of differentiating into liver and mesenchymal lineages. Proc Natl Acad Sci USA 103, 9912–9917.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Wu, Y., Shatapathy, C., Minger, S. (2009). Isolation, In Vitro Cultivation and Characterisation of Foetal Liver Cells. In: Dhawan, A., Hughes, R. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 481. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-201-4_15
Download citation
DOI: https://doi.org/10.1007/978-1-59745-201-4_15
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-172-1
Online ISBN: 978-1-59745-201-4
eBook Packages: Springer Protocols