Abstract
Cellular transplantation represents an alternative to liver transplantation for the treatment of end-stage liver disease and liver-based inborn errors of metabolism. In order for cellular transplantation to be successful, an optimal source of cells for transplantation needs to be identified and the molecular mechanisms regulating their engraftment, proliferation, and functional differentiation elucidated. Here we describe a detailed protocol for the isolation, selection, and transplantation into an injured adult rat liver of a defined population of late gestation fetal rat hepatocytes. Also described is the methodology for assessing the purity of the selected cells and the efficiency with which they repopulate the adult liver. Our approach provides an in vivo model to study the molecular pathways involved in liver repopulation.
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References
Kim WR, Lake JR, Smith JM, Skeans MA, Schladt DP, Edwards EB, Harper AM, Wainright JL, Snyder JJ, Israni AK, Kasiske BL (2015) OPTN/SRTR 2013 Annual Data Report: liver. Am J Transplant 15(Suppl 2):1–28. doi:10.1111/ajt.13197
Hansel MC, Gramignoli R, Skvorak KJ, Dorko K, Marongiu F, Blake W, Davila J, Strom SC (2014) The history and use of human hepatocytes for the treatment of liver diseases: the first 100 patients. Curr Protoc Toxicol 62:14.12.1–14.12.23. doi:10.1002/0471140856.tx1412s62
Thompson NL, Hixson DC, Callanan H, Panzica M, Flanagan D, Faris RA, Hong WJ, Hartel-Schenk S, Doyle D (1991) A Fischer rat substrain deficient in dipeptidyl peptidase IV activity makes normal steady-state RNA levels and an altered protein. Use as a liver-cell transplantation model. Biochem J 273(Pt 3):497–502
Brilliant KE, Mills DR, Callanan HM, Hixson DC (2009) Engraftment of syngeneic and allogeneic endothelial cells, hepatocytes and cholangiocytes into partially hepatectomized rats previously treated with mitomycin C. Transplantation 88(4):486–495. doi:10.1097/TP.0b013e3181b0b98a00007890-200908270-00008 [pii]
Mowery J, Hixson DC (1991) Detection of cell-CAM 105 in the pericanalicular domain of the rat hepatocyte plasma membrane. Hepatology 13(1):47–56
Rogler LE (1997) Selective bipotential differentiation of mouse embryonic hepatoblasts in vitro. Am J Pathol 150(2):591–602
Laconi E, Dabeva M, Laconi S, Pani P, Mukhopadhyay D, Oren R, Shafritz D (1997) Rapid proliferation of transplanted syngeneic DPPIV+ hepatocytes in the liver of pyrrolizidine alkaloid treated DPPIV-fisher rats. FASEB J 11:A104
Acknowledgments
We thank Heather Francois-Vaughan and Joan Boylan for careful reading of the chapter. This work was supported by National Institutes of Health grant R01DK100301 and by the Rhode Island Hospital Department of Pediatrics.
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Sanders, J.A. (2017). Late Gestation Fetal Hepatocytes for Liver Repopulation in the Rat. In: Stock, P., Christ, B. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 1506. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6506-9_3
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DOI: https://doi.org/10.1007/978-1-4939-6506-9_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6504-5
Online ISBN: 978-1-4939-6506-9
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