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Preparation of Functional Human Hepatocytes Ex Vivo

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Hepatocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2544))

Abstract

Hepatocytes are liver parenchymal cells involved in performing various metabolic reactions. During the development of therapeutic drugs, toxicological assays are conducted using hepatocyte cultures before clinical trials. However, since primary hepatocytes cannot proliferate and rapidly lose their functions in vitro, many efforts have been put into modifying culture conditions to expand primary hepatocytes and induce hepatocyte functions in intrinsic and extrinsic stem/progenitor cells. In this chapter, we summarize recent advances in preparing hepatocyte cultures and induction of hepatocytes from various cellular sources.

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Authors and Affiliations

  1. Division of Regenerative Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

    Ayumu Okumura & Naoki Tanimizu

  2. Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan

    Naoki Tanimizu

Authors
  1. Ayumu Okumura
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  2. Naoki Tanimizu
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Correspondence to Naoki Tanimizu .

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Editors and Affiliations

  1. Research Institute for Frontier Medicine, Sapporo Medical University, School of Medicine, Sapporo, Hokkaido, Japan

    Naoki Tanimizu

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Okumura, A., Tanimizu, N. (2022). Preparation of Functional Human Hepatocytes Ex Vivo. In: Tanimizu, N. (eds) Hepatocytes. Methods in Molecular Biology, vol 2544. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2557-6_20

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  • DOI: https://doi.org/10.1007/978-1-0716-2557-6_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2556-9

  • Online ISBN: 978-1-0716-2557-6

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