Abstract
Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.
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References
Soga T, Ohashi Y, Ueno Y et al (2003) Quantitative metabolome analysis using capillary electrophoresis mass spectrometry. J Proteome Res 2:488–494
Bedair M, Sumner LW (2008) Current and emerging mass-spectrometry technologies for metabolomics. Trends Anal Chem 27:238–250
Yoshida H, Mizukoshi T, Hirayama K et al (2007) Comprehensive analytical method for the determination of hydrophilic metabolites by high-performance liquid chromatography and mass spectrometry. J Agric Food Chem 55:551–560
Ishii N, Nakahigashi K, Baba T et al (2007) Multiple high-throughput analyses monitor the response of E. coli to perturbations. Science 316:593–597
Soga T, Igarashi K, Ito C et al (2009) Metabolomic profiling of anionic metabolites by capillary electrophoresis mass spectrometry. Anal Chem 81:6165–6174
Sato S, Soga T, Nishioka T et al (2004) Simultaneous determination of the main metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection. Plant J 40:151–163
Abiko T, Wakayama M, Kawakami A et al (2010) Changes in nitrogen assimilation, metabolism, and growth in transgenic rice plants expressing a fungal NADP(H)-dependent glutamate dehydrogenase (gdhA). Planta 232:299–311
Wakayama M, Aoki N, Sasaki H et al (2010) Simultaneous analysis of amino acids and carboxylic acids by capillary electrophoresis-mass spectrometry using an acidic electrolyte and uncoated fused-silica capillary. Anal Chem 82:9967–9976
Sugimoto M, Goto H, Otomo K et al (2010) Metabolomic profiles and sensory attributes of edamame under various storage duration and temperature conditions. J Agric Food Chem 58:8418–8425
Sugimoto M, Kaneko M, Onuma H et al (2012) Changes in the charged metabolite and sugar profiles of pasteurized and unpasteurized Japanese sake with storage. J Agric Food Chem 60:2586–2593
Soga T, Baran R, Suematsu M et al (2006) Differential metabolomics reveals ophthalmic acid as an oxidative stress biomarker indicating hepatic glutathione consumption. J Biol Chem 281:16768–16776
Hirayama A, Kami K, Sugimoto M et al (2009) Quantitative metabolome profiling of colon and stomach cancer microenvironment by capillary electrophoresis time-of-flight mass spectrometry. Cancer Res 69:4918–4925
Hirayama A, Nakashima E, Sugimoto M et al (2012) Metabolic profiling reveals new serum biomarkers for differentiating diabetic nephropathy. Anal Bioanal Chem 404:3101–3109
Takeuchi K, Ohishi M, Ota S et al (2013) Metabolic profiling to identify potential serum biomarkers for gastric ulceration induced by nonsteroid anti-inflammatory drugs. J Proteome Res 12:1399–1407
Ramautar R, Somsen GW, de Jong GJ (2013) CE-MS for metabolomics: developments and applications in the period 2010–2012. Electrophoresis 34:86–98
Wang X, Li K, Adams E et al (2013) Capillary electrophoresis-mass spectrometry in metabolomics: the potential for driving drug discovery and development. Curr Drug Metab 14:807–813
Soga T, Heiger DN (2000) Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry. Anal Chem 72:1236–1241
Soga T, Kakazu Y, Robert M et al (2004) Qualitative and quantitative analysis of amino acids by capillary electrophoresis-electrospray ionization-tandem mass spectrometry. Electrophoresis 25:1964–1972
Soga T, Ueno Y, Naraoka H et al (2002) Simultaneous determination of anionic intermediates for Bacillus subtilis metabolic pathways by capillary electrophoresis electrospray ionization mass spectrometry. Anal Chem 74:2233–2239
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Wakayama, M., Hirayama, A., Soga, T. (2015). Capillary Electrophoresis-Mass Spectrometry. In: Bjerrum, J. (eds) Metabonomics. Methods in Molecular Biology, vol 1277. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2377-9_9
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DOI: https://doi.org/10.1007/978-1-4939-2377-9_9
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