Abstract
Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.
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References
Smith JT (1999) Recent advancements in amino acid analysis using capillary electrophoresis. Electrophoresis 20:3078–3083
Han J, Danell RM, Patel JR, Gumerov DR, Scarlett CO, Speir JP, Parker CE, Rusyn I, Zeisel S, Borchers CH (2008) Towards high-throughput metabolomics using ultrahigh-field Fourier transform ion cyclotron resonance mass spectrometry. Metabolomics 4:128–140
Soga T, Heiger DN (2000) Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry. Anal Chem 72:1236–1241
Soga T, Ohashi Y, Ueno Y, Naraoka H, Tomita M, Nishioka T (2003) Quantitative metabolome analysis using capillary electrophoresis mass spectrometry. J Proteome Res 2:488–494
Hirayama A, Sugimoto M, Suzuki A, Hatakeyama Y, Enomoto A, Harada S, Soga T, Tomita M, Takebayashi T (2015) Effects of processing and storage conditions on charged metabolomic profiles in blood. Electrophoresis 36(18):2148–2155
Hirayama A, Tomita M, Soga T (2012) Sheathless capillary electrophoresis-mass spectrometry with a high-sensitivity porous sprayer for cationic metabolome analysis. Analyst 137:5026–5033
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Hirayama, A., Ikeda, S., Sato, A., Soga, T. (2019). Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry. In: Alterman, M. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 2030. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9639-1_23
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DOI: https://doi.org/10.1007/978-1-4939-9639-1_23
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9638-4
Online ISBN: 978-1-4939-9639-1
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