Although herbs, spices, medicinal, and nutraceutical plants have been used in human health for millennia, there has been renewed interest over the past number of years. In the past, people have relied on landraces or “wild” plants as there has been very little breeding or genetics done on these species. This is changing as consumers are demanding scientific evidence for the medical claims (clinical trials) as well as uniformity in the products. Doubled haploidy would be one way in which to achieve homozygous, true-breeding lines. Haploidy response, i.e. callus, embryos, regenerated haploid/doubled haploid plants, have been reported in a number of medicinal/nutraceutical species, however the frequency of response is low compared to other species. This review will focus on a few of the major plant families with medicinal properties.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
References
Badea E, Iordan M, Mihalea A (1985) Induction of androgenesis in anther culture of Digitalis lanata. Revue Roumaine de biologie. Serie de Biologie vegetale 30:63–71
Bajaj YPS (1978) Effect of super-low temperature on excised anthers and pollen-embryos of Atropa, Nicotiana and Petunia. Phytomorphology 2:171–176
Bapat VA, Wenzel G (1982) In vitro haploid plantlet induction in Physalis ixocarpa Brot. through microspore embryogenesis. Plant Cell Rep 1:154–156
Bicknell RABNK (1996) Isolation of reduced genotypes of Hieracium pilosella using anther culture. Plant Cell Tiss Org Cult 45:37–41
Bohanec B (1997) Haploid induction in buckwheat (Fagopyrum esculentum Moench). In Jain SM, Sopory SK, Veilleux RE (eds), In Vitro Haploid Production in Higher Plants. Kluwer, Dordrecht, 4:163–170
Bohanec B, Neskovic M, Vujicic R (1993) Anther culture and androgenetic plant regeneration in buckwheat (Fagopyrum esculentum Moench). Plant Cell Tiss Org Cult 35:259–266
Bugara AM (1986) Embryoidogenesis in anther culture of Salvia sclarea. Fiziologiia I Biokhimiia Kulturnykh Rastenii. Physiol Biochem Cultivated Plants 18:381–386
Chand S, Basu P (1998) Embryogenesis and plant regeneration from callus cultures derived from unpollinated ovaries of Hyoscyamus muticus L. Plant Cell Rep 17:302–305
Chand S, Sahrawat AK (2007) Embryogenesis and plant regeneration from unpollinated ovary culture of Psoralea corylifolia. Biologia Plantarum 51:223–228
Chaturvedi R, Razdan MK, Bhojwani SS (2003) Production of haploids of neem (Azadirachta indica A. Juss.) by anther culture. Plant Cell Rep 21:531–537
Corduan G (1976) Isozyme variation as an indicator for the generative or somatic origin of anther- derived plants of Digitalis purpurea L. Z. Pflanzenzuchtg 76:47–55
Corduan G, Spix S (1975) Haploid callus and regeneration of plants from anthers of Digitalis purpurea L. Planta 124:1–11
Diettrich B, Ernst S, Luckner M (2000) Haploid plants regenerated from androgenic cell cultures of Digitalis lanata. Planta Medica 66:237–240
Dieu P, Dunwell JM (1988) Anther culture with different genotypes of opium poppy (Papaver somniferum L.): effect of cold treatment. Plant Cell Tiss Org Cult 12:263–271
Ferrie AMR (2006) Doubled haploid production in nutraceutical species: a review. Euphytica 158:347–357
Ferrie AMR (2007) Developing double haploidy breeding methods for nutraceutical species. In Proceedings of The Natural Health Products Research Society of Canada and The Canadian Herb, Spice, and Natural Health Products Coalition Conference: Tradition to Technology, Saskatoon, Canada. May 10–13
Ferrie AMR, Bethune T, Kernan Z (2005) An overview of preliminary studies on the development of doubled haploid protocols for nutraceutical species. Acta Physiol Plant 27:735–741
George L (1985) Anther culture of Catharanthus roseus L.—development of pollen embryoids. Curr Sci 54:641–642
Govil CM, Singh VRR (1982) Induction of haploids in anther culture of Sesamum indicum. In Proceedings of the 5th International Congress Plant Tissue and Cell Culture. pp. 545–546
Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497
Guha S, Maheshwari SC (1966) Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Nature 212:97–98
Hatano K, Shoyama Y, Nishioka I (1987) Somatic embryogenesis and plant regeneration from the anther of Aconitum carmichaeli Debx. Plant Cell Rep 6:446–448
Iqbal MCM, Wijesekara KB (2007) A brief temperature pulse enhances the competency of micro- spores for androgenesis in Datura metel. Plant Cell Tiss Org Cult 89:141–149
Johansson L, Andersson B, Eriksson T (1982) Improvement of anther culture technique: activated charcoal bound in agar medium and elevated CO2 concentration. Physiol Plant 54:24–30
Kernan Z, Ferrie AMR (2006) Microspore embryogenesis and the development of a double haploidy protocol for cow cockle (Saponaria vaccaria). Plant Cell Rep 25:274–280
Laurain D, Trémouillaux-Guiller J, Chénieux J (1993) Embryogenesis from microspores of Ginkgo biloba L., a medicinal woody species. Plant Cell Rep 12:501–505
Martinez LD, de Halac IN (1995) Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries. Plant Cell Tiss Org Cult 42:91–96
Matsubara S, Dohya N, Murakami K (1995) Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Hortic 392:129–137
Nagmani R, Raghavan V (1983) Induction of embryogenic divisions in isolated pollen grains of Hyoscyamus niger in a single-step method. Z.Pflanzenphysiol 109:87–90
Nomizu T, Niimi Y, Han D-S (2004) Haploid plant regeneration via embryogenesis from anther cultures of Hepatica nobilis. Plant Cell Tiss Org Cult 79:307–313
Perez-Bermudez P, Cornejo MJ, Segura J (1985) Pollen plant formation from anther cultures of Digitalis obscura L. Plant Cell Tiss Org Cult 5:63–68
Prakash DVSSR, Chand S (1999) In vitro response from cultured anthers of Boswellia serrata Roxb. (Burseraceae). In Plant Tissue Culture and Biotechnology: Emerging trends. Universities Press, Hyderabad, India, pp. 226–231
Qiquan S, Anscheng L (1986) Somatic embryogenesis and plant regeneration from anther culture of Panax quinquifolius (Ginseng). Int Plant Biotech Network 6:2
Raghavan V (1978) Origin and development of pollen-embryoids and pollen calluses in cultured anther segments of Hyoscyamus niger (Henbane). Am J Bot 65:984–1002
Raghavan V, Nagmani R (1989) Cytokinin effects on pollen embryogenesis in cultured anthers of Hyoscyamus niger. Can J Bot 67:247–257
Safarova D, Kopecky D, Vagera J (2005) The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba. Biol Plantarum 49:261–264
Samsudden K, Babu KN, Divakaran M, Ravindran PN (2000) Plant regeneration from anther derived callus cultures of ginger (Zingiber officinale Rosc.). J Hortic Sci Biotechnol 75:447–450
Schulte J, Büter B, Schaffner W, Berger K (1996) Gametic embryogenesis in Hypericum spp. In Pank F (ed) International Symposium on Breeding Research on Medicinal and Aromatic Plants. Quedlinburg, Germany, pp. 307–310
Seran TH, Hirimburegama K, Hirimburegama WK, Shanmugarajah V (1999) Callus formation in anther cultures of tea clones, Camellia sinensis (L.) O. Kuntze. J Natl Sci Found Sri Lanka 27:165–175
Shon T-K, Kim S-K, Acquah D, Lee S-C (2004) Haploid plantlet production through somatic embryogenesis in anther-derived callus of Bupleurum falcatum. Plant Prod Sci 7:204–211
Shon T-K, Yoshida T (1997) Induction of haploid plantlets by anther culture of Bupleurum falca-tum L. Jpn J Crop Sci 66:137–138
Suh SK, Park HG (1986) Studies on the anther culture of garlic (Allium sativum L.). J Korean Soc Hortic Sci 27:89–95
Sunderland N, Wildon DC (1979) A note on the pretreatment of excised flower buds in float culture of Hyoscyamus anthers. Plant Sci Lett 15:169–175
Van Eck JM, Kitto SL (1990) Callus initiation and regeneration in Mentha. Hortscience 25:804–806
Zenkteler M (1971) In vitro production of haploid plants from pollen grains of Atropa belladonna L. Experientia 27:1087
Zhao F-C, Nilanthi D, Yang Y-H, Wu H (2006) Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.). Plant Cell Rep 86:55–62
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Ferrie, A.M.R. (2009). Current Status of Doubled Haploids in Medicinal Plants. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_18
Download citation
DOI: https://doi.org/10.1007/978-1-4020-8854-4_18
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-8853-7
Online ISBN: 978-1-4020-8854-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)