Abstract
Plants of reduced ploidy were derived from anther tissue of a pentaploid, apomictic biotype of Hieracium pilosella (mouse-ear hawkweed). Callusing was observed from both the somatic and generative tissue of the anther. Capitulum diameter was used as a correlative character to determine the best stage for harvesting the tissue. The highest degree of callusing coincided with the early uninucleate stage of microsporogenesis. Regenerants included tetraploids, triploids and two classes of aneuploids. Segregation for apomixis was observed among the tetraploid regenerants, an indication of the dominant inheritance of apomixis in this species.
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Abbreviations
- BA:
-
6-benzylaminopurine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
References
Asker SE & Jerling L (1992) Apomixis in Plants. CRC Press, Boca Raton
Bicknell RA (1994a) Hieracium; A model system for studying the molecular genetics of apomixis. Apomixis Newsletter. 7: 8–10
Bicknell RA (1994b) Micropropagation of Hieracium aurantiacum. Plant Cell Tiss. Org. Cult. In Press
Bohorova NA, Atanassov A, Georgie-Va-Todorova J (1985) In vitro organogenesis, androgenesis and embryo culture, in the genus Helianthus L. Z. Planzenzüchtg. 95: 35–44
Christoff M & Popoff A (1933) Cytologische Studien über die Gattung Hieracium. Planta. 20: 440–447
Darlington CD (1939) The evolution of genetic systems. Cambridge University Press, London
Dunwell JM (1985) Haploid cell cultures. In: Plant Cell Culture, A Practical Approach (pp 21–36). IRL Press, Oxford
Gadella TWJ (1972) Biosystematic studies in Hieraceum pilosella L. and some related species of the subgenus Pilosella. Bot. Notiser. 125: 361–369
Gadella TWJ (1991) Variation, hybridization and reproductive biology of Hieracium pilosella L. Proc. Kon. Ned. Akad. V. Wetensch. 94(4): 455–488
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Gurel A, Nichterlein K & Friedt W (1991) Shoot regeneration from anther culture of sunflower (Helianthus annuus) and some interspecific hybrids as affected by genotype and culture procedure. Plant Breed. 106: 68–76
Manton I (1950) Apogamous ferns: The general phenomenon. In: Problems of Cytology and Evolution in the Pteridophyta (pp 158–310) Cambridge University Press, Cambridge
Murashige T & Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nogler GA (1982) How to obtain diploid apomictic Ranunculus auricomus plants not found in the wild state. Bot. Helvet. 92: 13–22
Nogler GA (1984) Genetics of apospory in apomictic Ranunculus auricomus. V. Conclusion. Botan. Helvet. 94(2): 411–422
Richards AJ (1986) Plant Breeding Systems. George Allen & Unwin, London
Rosenberg O (1907) Experimental and cytological studies in the Hieracia. II. Cytological studies on the apogamy in Hieracium. Bot. Tids. 28: 143–170
Rutishauser A (1948) Pseudogamie und Polymorphie in der Gattung Potentilla. Arch. Julius-klaus-Stiftung f. Vererb.-Forsch. 23: 267–424
Skalinska M (1971) Experimental and embryological studies in Hieracium aurantiacum L. Acta Biol. Crac. ser. Bot. 14: 139–155
Thengane SR, Joshi MS, Khuspe SS & Mascarenahs AF (1994) Anther culture in Helianthus annuus L., influence of genotype and culture conditions on embryo induction and plant regeneration. Plant Cell Rep. 13: 222–226
Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM & Webb DA (1976) Flora Europaea. 4: 368–369
Wenzel G & Foroughi-Wehr B (1984) Anther culture of Solanum tuberosum. In: Cell Culture and Somatic Cell Genetics of Plants (pp 293–301). Academic Press, San Diego
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Bicknell, R.A., Borst, N.K. Isolation of reduced genotypes of Hieracium pilosella using anther culture. Plant Cell Tiss Organ Cult 45, 37–41 (1996). https://doi.org/10.1007/BF00043426
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DOI: https://doi.org/10.1007/BF00043426