Abstract
Anthers of O. hookeri containing uninucleate microspores were cultured, in vitro, at 25°C (16 hours photoperiod) on solid MS medium. After 10–15 days, on media with 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid and 6-benzylaminopurine, anthers developed friable calluses. After unsuccessful treatments on embryogenic-and/or organogenic-induction media, calluses were placed on a hormone-free MS medium for 24 months with routine transfers every 3 weeks. After this period, the calluses developed buds and subsequently plants. Ro generation plants, were morphologically distinct.
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Abbreviations
- BA:
-
6-benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxiacetic acid
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige & Skoog (1962) medium
- NAA:
-
1-naphthaleneacetic acid
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Martinez, L.D., de Halac, I.N. Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries. Plant Cell Tiss Organ Cult 42, 91–96 (1995). https://doi.org/10.1007/BF00037686
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DOI: https://doi.org/10.1007/BF00037686