Abstract
A low-cost Growtek bioreactor has been designed, patented and commercialised. It has unique features such as a floating and rotating explant-holder with perforated explant support and a side tube for medium changing, culture feeding and for content monitoring. The bioreactor can be operated both in static and agitated modes. Extensive performance studies have been conducted using representatives of trees (Santalum album), commercial ornamentals (Dendranthema grandiflora), monocotyledonous horticultural species (Ananas comosus), tuber crops (Solanum tuberosum) and a medicinal plant (Catharanthus roseus). In comparison to propagation in agar-gelled media as well as in liquid media using other culture vessels, this bioreactor exhibited 1.2 – 23.3 times shoot production, minimised root injuries by 32 – 48 %, reduced contamination by 12 – 18 % and reduced incubation time by 16– 42 %. Thousands of Ananas comosus plantlets raised in this bioreactor have been field tested. Additionally, it was found to be effective for hairy root culture of C. roseus.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
Key words
References
Bhattacharya PS, Dey S, Das N & Bhattacharyya BC (1990) Rapid mass propagation of Chrysanthemum morifolium by callus derived from stem and leaf explants. Plant Cell Rep. 9: 439–443
Bhattacharya P, Dey S & Bhattacharyya BC (1994) Use of low-cost gelling agents and support matrices for industrial scale plant tissue culture. Plant Cell Tiss. Org. Cult. 37: 15–23
Cassels AC (1991) Problems in tissue culture: contamination. In: PC Debergh and RH Zimmermann (eds) Micropropagation Technology and Application (pp. 31–44). Kluwer Academic Publishers, Dordrecht
Curtis WR & Emery AH (1993) Plant cell suspension culture rheology. Biotechnol. Bioengng. 42: 520–526
Das Susobhan, Das Surajit, Pal S, Mujib A, Sahoo SS, Dey S, Ponde NR & Das Gupta S (1999) A novel process for rapid mass propagation of Santalum album L. in liquid media and bioreactor. In: Giberti G et al. (eds) Agricultural Production, Post Harvest Techniques, Biotechnology (proc, WOCMAP2) Acta Hort. 522: 281–286
Debergh P (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol. Plant. 59: 270–276
Dey S (2001) Mass cloning of Santalum album L. through somatic embryogenesis: scale up in bioreactor. Sandalwood Research Newsletter 13: 1–3
Doran PM (2000) Foreign protein production in plant tissue cultures. Current Opinion in Biotechnology 11: 199–204
Escalona M, Lorenzo JC, Gonzalez B, Daquinta M, Gonzalez JL, Desjardins Y & Borroto CG (1999) Pineapple (Ananas comosus L. Merr.) micropropagation in temporary immersion systems. Plant Cell Rep. 18: 743–748
Etienne H, Lartaud M, Michaux-Ferriere N, Carron MP, Berthouly M & Teisson C (1997) Improvement of somatic embryogenesis in Hevea Brasiliensis (Mull. Arg.) using the temporary immersion technique. In Vitro Cell. Dev. Biol. 33: 81–87
Facchini PJ & Di Cosmo F (1991) Plant cell bioreactor for production of protoberberine alkaloids from immobilized Thalictrum rugosum cultures. Biotechnol. Bioengineering 37: 397–403
Gangopadhyay G, Das S, Mitra SK, Poddar R, Modak BK & Mukherjee KK (2002) Enhanced rate of multiplication and rooting through the use of coir in aseptic liquid culture media. Plant Cell, Tiss. Org. Cult. 68: 301–310
Goldstein WE (1999) Economic considerations for food ingredients produced by plant cell and tissue culture. In: Fu et al. (eds) Plant Cell and Tissue Culture for the Production of Food Ingredients (pp. 195–213). Kluwer Academic/Plenum Publishers, New York
Gupta PK, Pullman G, Timmis R, Kreitinger M, Carlson WC, Grob J & Welty E (1993) Forestry in 21st century: the biotechnology of somatic embryogenesis. Biotechnology 11: 454–459
Henderson WE & Kinnersley AM (1988) Corn starch as an alternative gelling agent for plant tissue culture. Plant Cell, Tiss. Org. Cult. 15: 17–22
Hunter CS & Kilby NJ (1999) Betalains: their accumulation and release in vitro. In: Robert D Hall (ed.) Plant Cell Culture Protocols. Methods in Molecular Biology, Vol. 111 (pp. 403–410), Humana Press Wageningen
Hvoslef-Eide AK & Melby TI (2000) Summary of results from bioreactor experiments with Cyclamen propagation via somatic embryogenesis. COST 843 Working Group 2 Meeting, Advanced Propagation Techniques (pp. 18–20). Tampere, Finland, July 7–10
Jimenez E, Perez N, de Feria M, Barbon R, Capote A, Chavez M & Quiala E (1999) Improved production of potato microtuber in a temporary immersion system. Plant Cell, Tiss. Org. Cult. 59: 19–23
Kavanagh K, Drew AP & Maynard C (1991) The effect of the culture vessel on micropropagation. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, High-Tech and Micropropagation-I, Vol. 17 (pp. 202–211), Springer-Verlag, Berlin, Heidelberg
Klerk Geert-Jan de (2001) Rooting of microcuttings: theory and practice. In Vitro Cell. Dev. Biol. (Plant) 37(3): p. 19 (Part-II)
Lakshmi Sita G, Singh R & Iyer CPA (1974) Plantlets through shoot tip cultures in pineapple. Curr. Sci. 43: 724–725
Maes M, Crepel C, Werbrouck S & Debergh P (1998) Perspectives for a DNA-based detection of bacterial contamination in micropropagated plant tissue. Plant Tissue Culture and Biotechnology 4: 49–56
Meyer JE, Pepin MF & Smith MAL (2002) Anthocyanin production from Vaccinium pahalae: limitations of physical microenvironment. Journal of Biotechnology 93: 45–57
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Peeters K, Wilde CD, Jaeger GD, Angenon G & Depicker A (2001) Production of antibodies and antibody fragments in plants. Vaccine 19: 2756–2761
Preil W (1991) Application of bioreactors in plant propagation. In: Debergh PC & Zimmermann RH (eds) Micopropagation: Technology and Application (pp. 425–445). Kluwer Academic Publishers, Dordrecht
Sharp JM & Doran PM (2001) Strategies for enhancing monoclonal antibody accummulation in plant cell and organ cultues. Biotechnol. Prog., 17: 979–992
Smith DR (1997) The role of in vitro methods in pine plantation establishment: the lesson from New Zealand. Plant Tissue Culture and Biotechnology 3: 63–73
Smith MAL & Spomer A (1995) Vessels, gels, liquid media and support systems. In: Aitken-Christie J, Kozai T & Smith L (eds) Automation and Environmental Control in Plant Tissue Culture (pp. 371–404). Kluwer Academic Publishers, Dordrecht
Soneji JR, Rao PS & Mhatre M (2002) Germination of synthetic seeds of pineapple (Ananas comosus L. Merr.) Plant Cell Rep. 20: 891–894
Sorvari S (1986) Comparison of anther cultures of barley cultivars in barley-starch and agar gelatinized media. Ann. Agric. Fenn. 25: 249–254
Stoger E, Sack M, Fischer R & Christou P (2002) Plantibodies: applications, advantages and bottlenecks. Current Opinion in Biotechnology. 13: 161–166
Sutton BCS & Polonendo (1999) Commercialisation of plant somatic embryogenesis. In: Jain SM, Gupta PK & Newton RJ (eds) Somatic Embryogenesis in Woody Plants (Forestry Sciences), Vol. 4 (pp. 263–291). Kluwer Academic Publishers, Dordrecht/Boston/London
Takayama S (1991) Mass propagation of plants through shake-and bioreactor-culture techniques. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, High-Tech and Micropropagation-I, Vol. 17 (pp. 495–515). Springer-Verlag, Berlin, Heidelberg
Tisserat B (1991) Automated systems. In: Y P S Bajaj (ed) Biotechnology in Agriculture Forestry, High-Tech and Micropropagation, Vol. 17 (pp. 420–431). Springer-Verlag, Berlin, Heidelberg
Vasil IK (1994) Automated plant propagation. Plant Cell, Tiss. Org. Cult. 39: 105–108
Zepeda C & Sagawa V (1981) In vitro propagation of pineapple. Hort. Sci. 16: 495–497
Zobayed SMA, Afreen F & Kozai T (2001) Physiology of Eucalyptus plantlets grown photo-autotrophically in a scaled-up vessel. In Vitro Cell. Dev. Biol (Plant) 37: 807–813
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer
About this chapter
Cite this chapter
Dey, S. (2005). Cost-effective mass cloning of plants in liquid media using a novel growtek bioreactor. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_8
Download citation
DOI: https://doi.org/10.1007/1-4020-3200-5_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3199-1
Online ISBN: 978-1-4020-3200-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)